{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T15:59:15Z","timestamp":1782316755693,"version":"3.54.5"},"reference-count":36,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T00:00:00Z","timestamp":1409270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The human body is exposed nowadays to increasing attacks by toxic compounds in polluted air, industrially processed foods, alcohol and drug consumption that increase liver toxicity, leading to more and more severe cases of hepatic disorders. The present paper aims to evaluate the influence of the apitherapy diet in Wistar rats with carbon tetrachloride-induced hepatotoxicity, by analyzing the biochemical determinations (enzymatic, lipid and protein profiles, coagulation parameters, minerals, blood count parameters, bilirubin levels) and histopathological changes at the level of liver, spleen and pancreas. The experiment was carried out on six groups of male Wistar rats. Hepatic lesions were induced by intraperitoneal injection of carbon tetrachloride (dissolved in paraffin oil, 10% solution). Two mL per 100 g were administered, every 2 days, for 2 weeks. Hepatoprotection was achieved with two apitherapy diet formulations containing honey, pollen, propolis, Apilarnil, with\/without royal jelly. Biochemical results reveal that the two apitherapy diet formulations have a positive effect on improving the enzymatic, lipid, and protein profiles, coagulation, mineral and blood count parameters and bilirubin levels. The histopathological results demonstrate the benefits of the two apitherapy diet formulations on reducing toxicity at the level of liver, spleen and pancreas in laboratory animals.<\/jats:p>","DOI":"10.3390\/molecules190913374","type":"journal-article","created":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T10:37:19Z","timestamp":1409308639000},"page":"13374-13391","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effect of Apitherapy Formulations against Carbon Tetrachloride-Induced Toxicity in Wistar Rats after Three Weeks of Treatment"],"prefix":"10.3390","volume":"19","author":[{"given":"Calin","family":"Andritoiu","sequence":"first","affiliation":[{"name":"Apitherapy Medical Center, Balanesti, Nr. 336-337, Gorj 217036, Romania"},{"name":"Gheorghe Asachi Technical University of Iasi, Department of Natural and Synthetic Polymers,  Iasi 700050, Romania"},{"name":"Vasile Goldis Western University of Arad, Liviu Rebreanu Street, 86, Arad 310045, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6447-0958","authenticated-orcid":false,"given":"Lacramioara","family":"Ochiuz","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy of Iasi, Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitatii Street, 16, Iasi 700115, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasile","family":"Andritoiu","sequence":"additional","affiliation":[{"name":"Apitherapy Medical Center, Balanesti, Nr. 336-337, Gorj 217036, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Popa","sequence":"additional","affiliation":[{"name":"Gheorghe Asachi Technical University of Iasi, Department of Natural and Synthetic Polymers,  Iasi 700050, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1078\/1438-4639-00175","article-title":"Industrial solvents and liver toxicity: Risk assessment, risk factors and mechanisms","volume":"205","author":"Brautbar","year":"2002","journal-title":"Int. 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