{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T15:40:44Z","timestamp":1762875644944,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2014,11,12]],"date-time":"2014-11-12T00:00:00Z","timestamp":1415750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Osteoclasts play a critical role in bone resorbing disorders such as osteoporosis, periodontitis, and rheumatoid arthritis. Therefore, discovery of agents capable of suppressing osteoclast differentiation may aid the development of a therapeutic access for the treatment of pathological bone loss. Ampelopsis brevipedunculata has been used as herbal folk medicine to treat liver diseases and inflammation in Asia. However, its effects on osteoclast differentiation are unknown. We were aimed to investigate the anti-osteoclastogenic activity in vitro and in vivo and to elucidate the underlying mechanism of Ampelopsis brevipedunculata extract (ABE). In this study, ABE inhibited receptor activator of NF-\u03baB ligand (RANKL)-induced osteoclast differentiation, the formation of filamentous actin rings and the bone resorbing activity of mature osteoclasts. ABE inhibited RANKL-induced p38 and I\u03baB phosphorylation and I\u03baB degradation. Also, ABE suppressed the mRNA and protein expression of nuclear factor of activated T cells c1 (NFATc1) and c-Fos, and the mRNA expression of genes required for cell fusion and bone resorption, such as  osteoclast-associated receptor (OSCAR), tartrate resistant acid phosphatase (TRAP), cathepsin K, dendritic cell-specific transmembrane protein (DC-STAMP), \u03b23-integrin and osteoclast stimulatory transmembrane protein (OC-STAMP). Furthermore, results of micro-CT and histologic analysis indicated that ABE remarkably prevented lipopolysaccharide (LPS)-induced bone erosion. These results demonstrate that ABE prevents LPS-induced bone erosion through inhibition of osteoclast differentiation and function, suggesting the promise of ABE as a potential cure for various osteoclast-associated bone diseases.<\/jats:p>","DOI":"10.3390\/molecules191118465","type":"journal-article","created":{"date-parts":[[2014,11,12]],"date-time":"2014-11-12T11:53:07Z","timestamp":1415793187000},"page":"18465-18478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Ampelopsis brevipedunculata Extract Prevents Bone Loss by Inhibiting Osteoclastogenesis in Vitro and in Vivo"],"prefix":"10.3390","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4493-0264","authenticated-orcid":false,"given":"Ju-Young","family":"Kim","sequence":"first","affiliation":[{"name":"Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun-Hyang","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea"},{"name":"BK21plus Program & Department of Smart Life-Care Convergence, Graduate School,  Wonkwang University, Iksan, Jeonbuk 570-749, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun","family":"Oh","sequence":"additional","affiliation":[{"name":"Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Jeonbuk 580-185, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung","family":"Kwak","sequence":"additional","affiliation":[{"name":"Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc.,  Seoul 130-831, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong","family":"Baek","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea"},{"name":"BK21plus Program & Department of Smart Life-Care Convergence, Graduate School,  Wonkwang University, Iksan, Jeonbuk 570-749, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myeung","family":"Lee","sequence":"additional","affiliation":[{"name":"Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Korea"},{"name":"Division of Rheumatology, Department of Internal Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea"},{"name":"Institute for Skeletal Disease, Wonkwang University, Iksan, Jeonbuk 570-749, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mun","family":"Rho","sequence":"additional","affiliation":[{"name":"Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Jeonbuk 580-185, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaemin","family":"Oh","sequence":"additional","affiliation":[{"name":"Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Korea"},{"name":"Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea"},{"name":"BK21plus Program & Department of Smart Life-Care Convergence, Graduate School,  Wonkwang University, Iksan, Jeonbuk 570-749, Korea"},{"name":"Institute for Skeletal Disease, Wonkwang University, Iksan, Jeonbuk 570-749, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,11,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"760","DOI":"10.3928\/01477447-20130523-21","article-title":"One-year mortality after isolated pelvic fractures with posterior ring involvement in elderly patients","volume":"36","author":"Bible","year":"2013","journal-title":"Orthopedics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.bone.2013.05.015","article-title":"Excess mortality after hip fracture among the elderly in Taiwan: A nationwide population-based cohort study","volume":"56","author":"Wang","year":"2013","journal-title":"Bone"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.bone.2006.09.023","article-title":"The molecular understanding of osteoclast differentiation","volume":"40","author":"Asagiri","year":"2007","journal-title":"Bone"},{"key":"ref_4","first-page":"1187","article-title":"RANKL signaling and bone diseases. 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