{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:08:42Z","timestamp":1762430922051,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T00:00:00Z","timestamp":1418601600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study focused on the assessment of the vasorelaxant activity of the organic and aqueous extracts obtained from leaves and fruits of a Mexican hawthorn (Crataegus gracilior) on isolated rat aorta, and on the purification and identification of some of their secondary metabolites by the use of chromatographic and spectroscopic techniques. The results obtained showed that the methanol extract has a significantly more potent and effective vasorelaxant effect than the other tested extracts, with an EC50 = 8.69 \u00b1 4.34 \u00b5g\/mL and  an Emax = 94.6% \u00b1 11.30%, values that are close to that of acetylcholine, the positive control. From the same extract, two major triterpenes were isolated and identified as ursolic and corosolic acids by comparison of their experimental NMR spectroscopic data with those reported in the literature. Chlorogenic acid, rutin, quercetin, kaempferol and (+)-catechin were also identified using HPLC coupled with PDAD. All these compounds have already been proven to possess on their own antihypertensive effect and other benefits on cardiovascular diseases and they can support, at least in part, the traditional use of this plant species.<\/jats:p>","DOI":"10.3390\/molecules191220962","type":"journal-article","created":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T10:25:19Z","timestamp":1418639119000},"page":"20962-20974","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Aortic Relaxant Activity of Crataegus gracilior Phipps and Identification of Some of Its Chemical Constituents"],"prefix":"10.3390","volume":"19","author":[{"given":"Abigail","family":"Hern\u00e1ndez-P\u00e9rez","sequence":"first","affiliation":[{"name":"Posgrado en Ciencias Qu\u00edmico Biol\u00f3gicas, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Centro Universitario, Cerro de las Campanas, Quer\u00e9taro 76010, Qro., Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moustapha","family":"Bah","sequence":"additional","affiliation":[{"name":"Posgrado en Ciencias Qu\u00edmico Biol\u00f3gicas, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Centro Universitario, Cerro de las Campanas, Quer\u00e9taro 76010, Qro., Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar","family":"Ibarra-Alvarado","sequence":"additional","affiliation":[{"name":"Posgrado en Ciencias Qu\u00edmico Biol\u00f3gicas, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Centro Universitario, Cerro de las Campanas, Quer\u00e9taro 76010, Qro., Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Rivero-Cruz","sequence":"additional","affiliation":[{"name":"Departamento de Farmacia, Facultad de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Circuito exterior, Coyoac\u00e1n 04510, Mexico D.F., Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra","family":"Rojas-Molina","sequence":"additional","affiliation":[{"name":"Posgrado en Ciencias Qu\u00edmico Biol\u00f3gicas, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Centro Universitario, Cerro de las Campanas, Quer\u00e9taro 76010, Qro., Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juana","family":"Rojas-Molina","sequence":"additional","affiliation":[{"name":"Posgrado en Ciencias Qu\u00edmico Biol\u00f3gicas, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Centro Universitario, Cerro de las Campanas, Quer\u00e9taro 76010, Qro., Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Cabrera-Luna","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Naturales, Universidad Aut\u00f3noma de Quer\u00e9taro, Campus Juriquilla,  Avenida de Las Ciencias S\/N, Delegaci\u00f3n Santa Rosa J\u00e1uregui, Quer\u00e9taro 76230, Qro., Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"World Health Organization A Global Brief on Hypertension: Silent Killer,Global Public Health Crisis. Available online: http:\/\/apps.who.int\/iris\/bitstream\/10665\/79059\/1\/WHO_DCO_WHD_2013.2_eng.pdf?ua=1.","DOI":"10.5005\/ijopmr-24-1-2"},{"key":"ref_2","unstructured":"Encuesta Nacional de Salud y Nutrici\u00f3n (ENSANUT) Evidencia Para la Pol\u00edtica P\u00fablica en Salud. Hipertensi\u00f3n Arterial en Adultos Mexicanos: Importancia de mejorar el diagn\u00f3stico Oportuno y el Control. Available online: http:\/\/ensanut.insp.mx\/doctos\/analiticos\/HypertensionArterialAdultos.pdf."},{"key":"ref_3","unstructured":"Pan American Health Organization (PAHO) Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure. Available online: http:\/\/www.nhlbi.nih.gov\/files\/docs\/guidelines\/jnc7full.pdf."},{"key":"ref_4","unstructured":"Atlas de las Plantas de la Medicina Tradicional Mexicana. Available online: http:\/\/www.medicinatradicionalmexicana.unam.mx\/monografia.php?l=3&t=&id=7778."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/s10557-006-8723-7","article-title":"Crataegus special extract WS1442 induces an endothelium-dependent, NO-mediated vasorelaxation via eNOS-phosphorylation at serine 1177","volume":"20","author":"Brixius","year":"2006","journal-title":"Cardiovasc. Drugs Ther."},{"key":"ref_6","first-page":"65","article-title":"Crataegus oxyacantha\u2014A cardioprotective herb","volume":"1","author":"Verma","year":"2007","journal-title":"J. Herb. Med. Toxicol. Chem."},{"key":"ref_7","first-page":"465","article-title":"Health Effects of Hawthorn","volume":"81","author":"Dahmer","year":"2010","journal-title":"Am. Fam. Phys."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.ejheart.2008.10.004","article-title":"The efficacy and safety of Crataegus extract WS\u00ae 1442 in patients with heart failure","volume":"10","author":"Holubarsch","year":"2008","journal-title":"Eur. J. Heart Fail."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"523","DOI":"10.2165\/00002018-200629060-00005","article-title":"Adverse-event profile of Crataegus spp.: A systematic review","volume":"29","author":"Daniele","year":"2006","journal-title":"Drug Safety"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.phytochem.2012.04.006","article-title":"A review of the chemistry of the genus Crataegus","volume":"79","author":"Edwards","year":"2012","journal-title":"Phytochemistry"},{"key":"ref_11","first-page":"177","article-title":"Distribuci\u00f3n y caracterizaci\u00f3n eco-clim\u00e1tica del g\u00e9nero Crataegus L. (Rosaceae, Subfam. Maloideae) en M\u00e9xico","volume":"14","author":"Segura","year":"2008","journal-title":"Rev. Chapingo Ser. Hortic."},{"key":"ref_12","unstructured":"Rzedowski, J., and Calderon-de-Rzedowski, G. (2005). Flora del Baj\u00edo y de Regiones Adyacentes. P\u00e1tzcuaro, Editorial INECOL Instituto de Ecolog\u00eda A.C.. Fasc\u00edculo 135."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/S0975-3575(10)80008-2","article-title":"Relaxant Effect of the Extracts of Crataegus mexicana on Guinea Pig Tracheal Smooth Muscle","volume":"2","author":"Arrieta","year":"2010","journal-title":"Pharmacogn. J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1080\/14786419.2012.704370","article-title":"Flavonoids and antioxidant activity of flowers of Mexican Crataegus spp.","volume":"27","year":"2013","journal-title":"Nat. Prod. Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Salvamani, S., Gunasekaran, B., Shaharuddin, N.A., Ahmad, S.Q., and Shukor, M.Y. (2014). Antiartherosclerotic effects of plant flavonoids. BioMed Res. Int., 2014.","DOI":"10.1155\/2014\/480258"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.3945\/jn.108.095554","article-title":"Chlorogenic acids from green coffee extract are highly bioavailable in humans","volume":"138","author":"Farah","year":"2008","journal-title":"J. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1016\/j.lfs.2006.03.006","article-title":"Ursolic acid mediates the vasorelaxant activity of Lepechinia caulescens via NO release in isolated rat thoracic aorta","volume":"79","year":"2006","journal-title":"Life Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0024-3205(00)00608-1","article-title":"Procyanidins in crataegus extract evoke endothelium-dependent vasorelaxation in rat aorta","volume":"67","author":"Kim","year":"2000","journal-title":"Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2474","DOI":"10.1016\/j.lfs.2006.08.007","article-title":"Corosolic acid prevents oxidative stress, inflammation and hypertension in SHR\/NDmcr-cp rats, a model of metabolic syndrome","volume":"79","author":"Yamaguchi","year":"2006","journal-title":"Life Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/sj.bjp.0704064","article-title":"Antihypertensive effects of the flavonoid quercetin in spontaneously hypertensive rats","volume":"133","author":"Duarte","year":"2001","journal-title":"Br. J. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s11010-005-4503-0","article-title":"Effects of chronic quercetin treatment in experimental renovascular hypertension","volume":"270","author":"Galisteo","year":"2005","journal-title":"Mol. Cell. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1097\/01.hjh.0000198029.22472.d9","article-title":"Quercetin downregulates NADPH oxidase, increases eNOS activity and prevents endothelial dysfunction in spontaneously hypertensive rats","volume":"24","author":"Galisteo","year":"2006","journal-title":"J. Hypertens."},{"key":"ref_23","first-page":"1146","article-title":"Efficiency of some antioxidants in reducing cardiometabolic risks in obese rats","volume":"7","author":"Abbass","year":"2011","journal-title":"J. Am. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"28","DOI":"10.4162\/nrp.2011.5.1.28","article-title":"Effects of daily quercetin rich supplementation on cardiometabolic risks in male smokers","volume":"5","author":"Lee","year":"2011","journal-title":"Nutr. Res. Pract."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.nut.2012.01.019","article-title":"Quercetin alleviates hypercholesterolemic diet induced inflammation during progression and regression of atherosclerosis in rabbits","volume":"29","author":"Bhaskar","year":"2013","journal-title":"Nutrition"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.imbio.2010.07.011","article-title":"Quercetin regulates oxidized LDL induced inflammatory changes in humanPBMCs by modulating the TLR-NF-\ud835\udf05B signaling pathway","volume":"216","author":"Bhaskar","year":"2011","journal-title":"Immunobiology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1002\/mrc.1214","article-title":"Complete assignments of 1H and 13C-NMR resonances of oleanolic acid, 18\u03b1-oleanolic acid, ursolic acid and their 11-oxo derivatives","volume":"41","author":"Seebacher","year":"2003","journal-title":"Magn. Reson. Chem."},{"key":"ref_28","first-page":"346","article-title":"Corosolic acid isolation from the leaves of Eriobotrta japonica showing the effects on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes","volume":"16","author":"Wei","year":"2007","journal-title":"Asia Pac. J. Clin. Nutr."},{"key":"ref_29","unstructured":"Norma Oficial Mexicana NOM-062-ZOO-1999, Especificaciones T\u00e9cnicas Para La Producci\u00f3n, Cuidado y Uso de Los Animales de Laboratorio. Available online: http:\/\/www.fmvz.unam.mx\/fmvz\/principal\/archivos\/062ZOO.PDF."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"732","DOI":"10.3109\/13880200903271280","article-title":"Vasoactive and antioxidant activities of plants used in Mexican traditional medicine for the treatment of cardiovascular diseases","volume":"48","author":"Rojas","year":"2010","journal-title":"Pharm. Biol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/19\/12\/20962\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:11:04Z","timestamp":1760217064000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/19\/12\/20962"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,15]]},"references-count":30,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2014,12]]}},"alternative-id":["molecules191220962"],"URL":"https:\/\/doi.org\/10.3390\/molecules191220962","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2014,12,15]]}}}