{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:00:11Z","timestamp":1791194411750,"version":"4.1.0"},"reference-count":137,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T00:00:00Z","timestamp":1418601600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Amoebiasis caused by Entamoeba histolytica is associated with high morbidity and mortality is becoming a major public health problem worldwide, especially in developing countries. Because of the side-effects and the resistance that pathogenic protozoa build against the standard antiparasitic drugs, e.g., metronidazole, much recent attention has been paid to plants used in traditional medicine around the world in order to find new antiprotozoal agents. We collected 32 plants used in Northeast Mexican traditional medicine and the methanolic extracts of these species were screened for antiprotozoal activity against E. histolytica trophozoites using in vitro tests. Only 18 extracts showed a significant inhibiting activity and among them six plant extracts showed more than 80% growth inhibition against E. histolytica at a concentration of 150 \u00b5g\/mL and the IC50 values of these extracts were determined. Lippia graveolens Kunth and Ruta chalepensis Pers. showed the more significant antiprotozoal activity (91.54% and 90.50% growth inhibition at a concentration of 150 \u00b5g\/mL with IC50 values of 59.14 and 60.07 \u00b5g\/mL, respectively). Bioassay-guided fractionation of the methanolic extracts from these two plants  afforded carvacrol (1) and chalepensin (2), respectively, as bioactive compounds with antiprotozoal activity.<\/jats:p>","DOI":"10.3390\/molecules191221044","type":"journal-article","created":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T10:25:19Z","timestamp":1418639119000},"page":"21044-21065","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Antiprotozoal Activity against Entamoeba histolytica of Plants Used in Northeast Mexican Traditional Medicine. Bioactive Compounds from Lippia graveolens and Ruta chalepensis"],"prefix":"10.3390","volume":"19","author":[{"given":"Ramiro","family":"Quintanilla-Licea","sequence":"first","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, UANL, Facultad de Ciencias Biol\u00f3gicas, Av. Universidad S\/N, Cd. Universitaria, San Nicol\u00e1s de los Garza, C.P. 66451 Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benito","family":"Mata-C\u00e1rdenas","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, UANL, Facultad de Ciencias Qu\u00edmicas, Av. Universidad S\/N, Cd. Universitaria, San Nicol\u00e1s de los Garza, C.P. 66451 Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Vargas-Villarreal","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica y Biolog\u00eda Celular, Centro de Investigaciones Biom\u00e9dicas del Noreste (CIBIN), Dos de abril esquina con San Luis Potos\u00ed, C.P. 64720 Monterrey, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo","family":"Bazald\u00faa-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, UANL, Facultad de Ciencias Biol\u00f3gicas, Av. Universidad S\/N, Cd. Universitaria, San Nicol\u00e1s de los Garza, C.P. 66451 Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isvar","family":"Kavimngeles-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, UANL, Facultad de Ciencias Biol\u00f3gicas, Av. Universidad S\/N, Cd. Universitaria, San Nicol\u00e1s de los Garza, C.P. 66451 Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Garza-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica y Biolog\u00eda Celular, Centro de Investigaciones Biom\u00e9dicas del Noreste (CIBIN), Dos de abril esquina con San Luis Potos\u00ed, C.P. 64720 Monterrey, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Hern\u00e1ndez-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica y Biolog\u00eda Celular, Centro de Investigaciones Biom\u00e9dicas del Noreste (CIBIN), Dos de abril esquina con San Luis Potos\u00ed, C.P. 64720 Monterrey, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1002\/ptr.3233","article-title":"Antiprotozoal activity of Melampyrum arvense and its metabolites","volume":"25","author":"Kirmizibekmez","year":"2011","journal-title":"Phytother. 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