{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:20:16Z","timestamp":1790187616905,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2014,12,24]],"date-time":"2014-12-24T00:00:00Z","timestamp":1419379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Scientific Foundation","award":["81302627"],"award-info":[{"award-number":["81302627"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Oxidative stress plays an important role in Parkinson\u2019s disease and other neurodegenerative disorders. Lycium barbarum polysaccharides (LBP), the main active ingredients extracted from the fruits of Lycium barbarum L., have been shown to be a potent antioxidant. In the present study, we investigated the protective effects, and the possible mechanism of action of LBP against 6-hydroxydopamine (6-OHDA)-induced apoptosis in PC12 cells. Our data demonstrated that LBP significantly reversed the 6-OHDA-induced decrease in cell viability, prevented 6-OHDA-induced changes in condensed nuclei and decreased the percentage of apoptotic cells in a dose-dependent manner. Furthermore, LBP also slowed the accumulation of reactive oxygen species (ROS) and nitric oxide (NO), decreased the level of protein-bound 3-nitrotyrosine (3-NT) and intracellular free Ca2+,  and inhibiting the overexpression of nuclear factor \u03baB (NF-\u03baB), neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS). These results demonstrate that LBP prevents 6-OHDA-induced apoptosis in PC12 cells, at least in part through the  ROS-NO pathway.<\/jats:p>","DOI":"10.3390\/molecules20010293","type":"journal-article","created":{"date-parts":[[2014,12,24]],"date-time":"2014-12-24T11:51:41Z","timestamp":1419421901000},"page":"293-308","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Protective Effects of Lycium barbarum Polysaccharide on  6-OHDA-Induced Apoptosis in PC12 Cells through the  ROS-NO Pathway"],"prefix":"10.3390","volume":"20","author":[{"given":"Kai","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiyou","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyi","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minna","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunyang","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University,  Xi'an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiankang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohe","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aidong","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/S1474-4422(06)70471-9","article-title":"Epidemiology of Parkinson\u2019s disease","volume":"5","author":"Breteler","year":"2006","journal-title":"Lancet Neurol."},{"key":"ref_2","first-page":"19","article-title":"Biochemical pathophysiology of Parkinson\u2019s disease","volume":"45","author":"Hornykiewicz","year":"1987","journal-title":"Adv. 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