{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:40:23Z","timestamp":1790617223869,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T00:00:00Z","timestamp":1428451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Biological Resources of the Ministry of Environment","award":["The Establishment and Management of Natural Products Bank derived from Bioresources"],"award-info":[{"award-number":["The Establishment and Management of Natural Products Bank derived from Bioresources"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The mouth cavity hosts many types of anaerobic bacteria, including Streptococcus mutans and Porphyromonas gingivalis, which cause periodontal inflammatory diseases and dental caries. The present study was conducted to evaluate the antibacterial potential of extracts of Robinia pseudoacacia and its different fractions, as well as some of its natural compounds against oral pathogens and a nonpathogenic reference bacteria,  Escherichia coli. The antibacterial activity of the crude extract and the solvent fractions (hexane, chloroform, ethyl acetate and butanol) of R. pseudoacacia were evaluated against S. mutans, P. gingivalis and E. coli DH5\u03b1 by standard micro-assay procedure using conventional sterile polystyrene microplates. The results showed that the crude extract was more active against P. gingivalis (100% growth inhibition) than against S. mutans (73% growth inhibition) at 1.8 mg\/mL. The chloroform and hexane fractions were active against P. gingivalis, with 91 and 97% growth inhibition, respectively, at 0.2 mg\/mL. None of seven natural compounds found in R. pseudoacacia exerted an antibacterial effect on  P. gingivalis; however, fisetin and myricetin at 8 \u00b5g\/mL inhibited the growth of S. mutans by 81% and 86%, respectively. The crude extract of R. pseudoacacia possesses bioactive compounds that could completely control the growth of P. gingivalis. The antibiotic activities of the hexane and chloroform fractions suggest that the active compounds are hydrophobic in nature. The results indicate the effectiveness of the plant in clinical applications for the treatment of dental plaque and periodontal inflammatory diseases and its potential use as disinfectant for various surgical and orthodontic appliances.<\/jats:p>","DOI":"10.3390\/molecules20046128","type":"journal-article","created":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T11:23:46Z","timestamp":1428492226000},"page":"6128-6139","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Bactericidal Effect of Extracts and Metabolites of  Robinia pseudoacacia L. on Streptococcus mutans and Porphyromonas gingivalis Causing Dental Plaque and Periodontal Inflammatory Diseases"],"prefix":"10.3390","volume":"20","author":[{"given":"Jayanta","family":"Patra","sequence":"first","affiliation":[{"name":"School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Wildlife Genetic Resources Center, National Institute of Biological Resources, Incheon 404-708, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyounghee","family":"Oh","sequence":"additional","affiliation":[{"name":"Wildlife Genetic Resources Center, National Institute of Biological Resources, Incheon 404-708, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeon-Jeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Ensoltek Co., Ltd., Techno 10-ro 51, Yuseong-gu, Daejeon 305-510, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radhika","family":"Dhakal","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangseon","family":"Kim","sequence":"additional","affiliation":[{"name":"Ensoltek Co., Ltd., Techno 10-ro 51, Yuseong-gu, Daejeon 305-510, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Hyun","family":"Baek","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,4,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.4236\/abb.2013.48A2003","article-title":"Antimicrobial effect of Anacardium occidentale leaf extract against pathogens causing periodontal disease","volume":"4","author":"Varghese","year":"2013","journal-title":"Adv. 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