{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:42:29Z","timestamp":1788964949061,"version":"build-2803163510"},"reference-count":28,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2015,5,20]],"date-time":"2015-05-20T00:00:00Z","timestamp":1432080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Emerging yeasts are among the most prevalent causes of systemic infections with high mortality rates and there is an urgent need to develop specific, effective and non-toxic antifungal agents to respond to this issue. In this study 35 aldehydes, hydrazones and hydrazines were obtained and their antifungal activity was evaluated against Candida species (C. parapsilosis, C. tropicalis, C. krusei, C. albicans, C. glabrata and C. lusitaneae) and Trichosporon asahii, in an in vitro screening. The minimum inhibitory concentrations (MICs) of the active compounds in the screening was determined against 10 clinical isolates of C. parapsilosis and 10 of T. asahii. The compounds 4-pyridin-2-ylbenzaldehyde] (13a) and tert-butyl-(2Z)-2-(3,4,5-trihydroxybenzylidine)hydrazine carboxylate (7b) showed the most promising MIC values in the range of 16\u201332 \u03bcg\/mL and 8\u201316 \u03bcg\/mL, respectively. The compounds\u2019 action on the stability of the cell membrane and cell wall was evaluated, which suggested the action of the compounds on the fungal cell membrane. Cell viability of leukocytes and an alkaline comet assay were performed to evaluate the cytotoxicity. Compound 13a was not cytotoxic at the active concentrations. These results support the discovery of promising candidates for the development of new antifungal agents.<\/jats:p>","DOI":"10.3390\/molecules20059229","type":"journal-article","created":{"date-parts":[[2015,5,20]],"date-time":"2015-05-20T11:05:46Z","timestamp":1432119946000},"page":"9229-9241","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Synthesis and Biological Evaluation of Hydrazone Derivatives as Antifungal Agents"],"prefix":"10.3390","volume":"20","author":[{"given":"Bruna","family":"Casanova","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Fitoqu\u00edmica e S\u00edntese Org\u00e2nica (LAFIS), Faculdade de Farm\u00e1cia,  Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Muniz","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Fitoqu\u00edmica e S\u00edntese Org\u00e2nica (LAFIS), Faculdade de Farm\u00e1cia,  Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thayse","family":"De Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia Aplicada, Departamento de Analises, Faculdade de Farm\u00e1cia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds","family":"De Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia, Universidade Federal do Pampa, Km 592, Uruguaiana 97500-970, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Machado","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia, Universidade Federal do Pampa, Km 592, Uruguaiana 97500-970, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Fuentefria","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia Aplicada, Departamento de Analises, Faculdade de Farm\u00e1cia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Gosmann","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Fitoqu\u00edmica e S\u00edntese Org\u00e2nica (LAFIS), Faculdade de Farm\u00e1cia,  Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Gnoatto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Fitoqu\u00edmica e S\u00edntese Org\u00e2nica (LAFIS), Faculdade de Farm\u00e1cia,  Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1097\/MAJ.0b013e3181e99c88","article-title":"Opportunistic fungi: A view to the future","volume":"340","author":"Pappas","year":"2010","journal-title":"Am. 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