{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:57:04Z","timestamp":1787029024176,"version":"build-2736575974"},"reference-count":63,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2015,6,22]],"date-time":"2015-06-22T00:00:00Z","timestamp":1434931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Ipecac alkaloids are secondary metabolites produced in the medicinal plant Psychotria ipecacuanha. Emetine is the main alkaloid of ipecac and one of the active compounds in syrup of Ipecac with emetic property. Here we evaluated emetine\u2019s potential as an antiviral agent against Human Immunodeficiency Virus. We performed in vitro Reverse Transcriptase (RT) Assay and Natural Endogenous Reverse Transcriptase Activity Assay (NERT) to evaluate HIV RT inhibition. Emetine molecular docking on HIV-1 RT was also analyzed. Phenotypic assays were performed in non-lymphocytic and in Peripheral Blood Mononuclear Cells (PBMC) with HIV-1 wild-type and HIV-harboring RT-resistant mutation to Nucleoside Reverse Transcriptase Inhibitors (M184V). Our results showed that HIV-1 RT was blocked in the presence of emetine in both models: in vitro reactions with isolated HIV-1 RT and intravirion, measured by NERT. Emetine revealed a strong potential of inhibiting HIV-1 replication in both cellular models, reaching 80% of reduction in HIV-1 infection, with low cytotoxic effect. Emetine also blocked HIV-1 infection of RT M184V mutant. These results suggest that emetine is able to penetrate in intact HIV particles, and bind and block reverse transcription reaction, suggesting that it can be used as anti-HIV microbicide. Taken together, our findings provide additional pharmacological information on the potential therapeutic effects of emetine.<\/jats:p>","DOI":"10.3390\/molecules200611474","type":"journal-article","created":{"date-parts":[[2015,6,22]],"date-time":"2015-06-22T12:06:06Z","timestamp":1434974766000},"page":"11474-11489","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Natural Plant Alkaloid (Emetine) Inhibits HIV-1 Replication by Interfering with Reverse Transcriptase Activity"],"prefix":"10.3390","volume":"20","author":[{"given":"Ana","family":"Valad\u00e3o","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Virologia Molecular, Departamento de Gen\u00e9tica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, Pr\u00e9dio CCS Bloco A, sala 121, 2\u00ba andar, CEP: 21941-902, Ilha do Fund\u00e3o, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celina","family":"Abreu","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Molecular, Departamento de Gen\u00e9tica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, Pr\u00e9dio CCS Bloco A, sala 121, 2\u00ba andar, CEP: 21941-902, Ilha do Fund\u00e3o, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Dias","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Molecular, Departamento de Gen\u00e9tica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, Pr\u00e9dio CCS Bloco A, sala 121, 2\u00ba andar, CEP: 21941-902, Ilha do Fund\u00e3o, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Arantes","sequence":"additional","affiliation":[{"name":"Grupo de Bioinform\u00e1tica Estrutural, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gon\u00e7alves 9500, CEP: 91500-970"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Verli","sequence":"additional","affiliation":[{"name":"Grupo de Bioinform\u00e1tica Estrutural, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gon\u00e7alves 9500, CEP: 91500-970"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amilcar","family":"Tanuri","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Molecular, Departamento de Gen\u00e9tica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, Pr\u00e9dio CCS Bloco A, sala 121, 2\u00ba andar, CEP: 21941-902, Ilha do Fund\u00e3o, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"De Aguiar","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Molecular, Departamento de Gen\u00e9tica, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho 373, Pr\u00e9dio CCS Bloco A, sala 121, 2\u00ba andar, CEP: 21941-902, Ilha do Fund\u00e3o, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,6,22]]},"reference":[{"key":"ref_1","unstructured":"(2014). 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