{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:40:52Z","timestamp":1790844052710,"version":"4.1.0"},"reference-count":26,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T00:00:00Z","timestamp":1437696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013704","name":"CONACYT","doi-asserted-by":"publisher","award":["CB 2010-156755"],"award-info":[{"award-number":["CB 2010-156755"]}],"id":[{"id":"10.13039\/501100013704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The plant Aeschynomene fascicularis (Fabaceae) has been used in Mayan traditional medicine in the Yucatan peninsula. However, the compounds present in the plant responsible for its curative properties have not yet been investigated. Aeschynomene fascicularis root bark was extracted with 100% methanol to obtain a crude extract. The methanol extract was partitioned successively with solvents with increasing polarity to obtain the corresponding hexane (Hx), dichloromethane (DCM) and ethyl acetate fractions (EtOAc), as well as a residual water-alcoholic fraction. These fractions were tested for their cytotoxic activities using an MTT assay against Hep-2 cancer cell lines. The Hx fraction led to the isolation  of spinochalcone C (1), spinochalcone A (2), isocordoin (3) and secundiflorol G (4). Their structures were identified based on spectroscopic evidence and chemical properties. All compounds were subjected to cytotoxicity and antiproliferative assays against a panel of seven cell lines, including one normal-type cell line. Spinochalcone A (2) exhibited cytotoxic activity against DU-145 cell line and antiproliferative activity against the KB cell line. Secundiflorol G (4) showed strong cytotoxic activity towards KB and Hep-2 cell lines. In addition, isocordoin (3) showed moderate activity on KB, Hep-2 and DU-145 cell lines. The active Compounds 2, 3 and 4 are potential therapeutic entities against cancer.<\/jats:p>","DOI":"10.3390\/molecules200813563","type":"journal-article","created":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T10:44:26Z","timestamp":1437734666000},"page":"13563-13574","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Isolation and Identification of Cytotoxic Compounds from Aeschynomene fascicularis, a Mayan Medicinal Plant"],"prefix":"10.3390","volume":"20","author":[{"given":"Edgar","family":"Caamal-Fuentes","sequence":"first","affiliation":[{"name":"Unidad de Biotecnolog\u00eda, Centro de Investigaci\u00f3n Cient\u00edfica de Yucat\u00e1n (CICY),  Calle 43 No. 130, Col. Chuburn\u00e1 de Hidalgo, M\u00e9rida, Yucat\u00e1n 97200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8161-501X","authenticated-orcid":false,"given":"Sergio","family":"Peraza-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Unidad de Biotecnolog\u00eda, Centro de Investigaci\u00f3n Cient\u00edfica de Yucat\u00e1n (CICY),  Calle 43 No. 130, Col. Chuburn\u00e1 de Hidalgo, M\u00e9rida, Yucat\u00e1n 97200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Torres-Tapia","sequence":"additional","affiliation":[{"name":"Unidad de Biotecnolog\u00eda, Centro de Investigaci\u00f3n Cient\u00edfica de Yucat\u00e1n (CICY),  Calle 43 No. 130, Col. Chuburn\u00e1 de Hidalgo, M\u00e9rida, Yucat\u00e1n 97200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Moo-Puc","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica Yucat\u00e1n, Unidad M\u00e9dica de Alta Especialidad,  Centro M\u00e9dico Ignacio Garc\u00eda T\u00e9llez, Instituto Mexicano del Seguro Social (IMSS),  Calle 41 No. 439, Col. Industrial, M\u00e9rida, Yucat\u00e1n 97150, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1111\/j.1600-0587.2009.06293.x","article-title":"The Yucatan peninsula: Biogeographical history 65 million years in the making","volume":"33","author":"Arita","year":"2010","journal-title":"Ecography"},{"key":"ref_2","unstructured":"Osadao, R. (1834). El Libro del Jud\u00edo o Medicina Dom\u00e9stica, Descripci\u00f3n de las Virtudes de las Yerbas Medicinales de Yucat\u00e1n, Dra. Dorothy Andrew de Zapata. [2nd ed.]."},{"key":"ref_3","unstructured":"Flores, J., and Espejel-Carvajal, I. (1994). Etnoflora Yucatanense: Tipos de Vegetaci\u00f3n de la Pen\u00ednsula de Yucat\u00e1n, Universidad Aut\u00f3noma de Yucat\u00e1n, Sostenibilidad Maya. Fasc\u00edculo 3."},{"key":"ref_4","unstructured":"Flores, J. (2001). Etnoflora Yucatanense: Leguminoseae. Flor\u00edstica, Etnobot\u00e1nica y Ecolog\u00eda, Universidad Aut\u00f3noma de Yucat\u00e1n."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1021\/np200906s","article-title":"Natural Products as Sources of New Drugs over the 30 Years from 1981 to 2010","volume":"75","author":"Newman","year":"2012","journal-title":"J. Nat. Prod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/j.jep.2011.04.004","article-title":"Screening of plants used in Mayan traditional medicine to treat cancer-like symptoms","volume":"135","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1021\/np960052v","article-title":"Two new aromatic constituents from the rootwood of Aeschynomene mimosifolia","volume":"59","author":"Fullas","year":"1996","journal-title":"J. Nat. Prod."},{"key":"ref_8","first-page":"1421","article-title":"Pterocarpans from the root bark of Aeschynomene fascicularis","volume":"8","year":"2013","journal-title":"Nat. Prod. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/0031-9422(92)80129-3","article-title":"Prenylated flavonoids from Tephrosia spinosa","volume":"32","author":"Rao","year":"1992","journal-title":"Phytochemistry"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1016\/0031-9422(92)80376-P","article-title":"Two chalcones from Tephrosia spinosa","volume":"31","author":"Rao","year":"1992","journal-title":"Phytochemistry"},{"key":"ref_11","first-page":"771","article-title":"C- and O-prenylated chalcones from Cordoa piaca","volume":"103","year":"1973","journal-title":"Gazz. Chim. Ital."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/S0031-9422(02)00131-0","article-title":"Flavonoids from two Lonchocarpus species of the Yucatan Peninsula","volume":"60","author":"Waterman","year":"2002","journal-title":"Phytochemistry"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1016\/S0031-9422(97)00802-9","article-title":"Isoflavonoids from Sophora secundiflora, S. arizonica and S. gypsophila","volume":"48","author":"Tanaka","year":"1998","journal-title":"Phytochemistry"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.phymed.2006.11.027","article-title":"Cytotoxic and antiprotozoal activity of flavonoids from Lonchocarpus spp.","volume":"14","author":"Balnbury","year":"2007","journal-title":"Phytomedicine"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1055\/s-0029-1185670","article-title":"Antiprotozoal and cytotoxic studies on some isocordoin derivatives","volume":"75","year":"2009","journal-title":"Planta Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.fitote.2011.02.006","article-title":"Malaysianol A, a new trimer resveratrol oligomer from the stem bark of Dryobalanops aromatica","volume":"82","author":"Wibowo","year":"2011","journal-title":"Fitoterapia"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0378-8741(03)00144-2","article-title":"In vitro antiplasmodial activity and cytotoxicity of ethnobotanically selected Ivorian plants","volume":"87","author":"Ouattara","year":"2003","journal-title":"J. Ethnopharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1021\/np970586b","article-title":"Cytotoxic isoflavans from Eysenhardtia polystachya","volume":"61","author":"Alvarez","year":"1998","journal-title":"J. Nat. Prod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"375","DOI":"10.3839\/jksabc.2009.067","article-title":"Antitumor components isolated from the heartwood extract of Dalbergia odorifera","volume":"52","author":"Choi","year":"2009","journal-title":"J. Korean Soc. Appl. Biol. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.cbi.2004.04.004","article-title":"Molecular cytotoxic mechanisms of anticancer hydroxychalcones","volume":"148","author":"Sabzevari","year":"2004","journal-title":"Chem. -Biol. Interact."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1248\/bpb.28.1404","article-title":"Xanthoangelol, a major chalcone constituent of Angelica keiskei, induces apoptosis in neuroblastoma and leukemia cells","volume":"28","author":"Tabata","year":"2005","journal-title":"Biol. Pharm. Bull."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1158\/0008-5472.CAN-04-3803","article-title":"Flavokawain A, a novel chalcone from kava extract, induces apoptosis in bladder cancer cells by involvement of Bax protein-dependent and mitochondria-dependent apoptotic pathway and suppresses tumor growth in mice","volume":"65","author":"Zi","year":"2005","journal-title":"Cancer Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1248\/bpb.30.1878","article-title":"Isobavachalcone, a chalcone constituent of Angelica keiskei, induces apoptosis in neuroblastoma","volume":"30","author":"Nishimura","year":"2007","journal-title":"Biol. Pharm. Bull."},{"key":"ref_24","unstructured":"Rahman, A., Choudhary, M.L., and Thonsen, W.J. (2001). Bioassay Techniques for Drug Development, Harwood Academic Publishers."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1093\/jnci\/82.13.1107","article-title":"New colorimetric cytotoxicity assay for anticancer-drug screening","volume":"82","author":"Skehan","year":"1990","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_26","first-page":"462","article-title":"In vitro cytotoxic activity of nine plants used in Mayan traditional medicine","volume":"21","year":"2009","journal-title":"J. Ethnopharmacol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/20\/8\/13563\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:49:40Z","timestamp":1760215780000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/20\/8\/13563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,24]]},"references-count":26,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2015,8]]}},"alternative-id":["molecules200813563"],"URL":"https:\/\/doi.org\/10.3390\/molecules200813563","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,24]]}}}