{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:57:52Z","timestamp":1787029072973,"version":"build-2736575974"},"reference-count":62,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T00:00:00Z","timestamp":1438646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004515","name":"Universiti Kebangsaan Malaysia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004515","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The ever-increasing threat from infectious diseases and the development of insecticide resistance in mosquito populations drive the global search for new natural insecticides. The aims of this study were to evaluate the mosquitocidal activity of the extracts of seaweed Bryopsis pennata against dengue vectors Aedes aegypti and Aedes albopictus, and determine the seaweed\u2019s toxic effect on brine shrimp nauplii (as a non-target organism). In addition, the chemical compositions of the active larvicidal extract and fraction were analyzed by using liquid chromatography-mass spectrometry (LC-MS). Chloroform extract exhibited strong ovicidal activity (with LC50 values of 229.3 and 250.5 \u00b5g\/mL) and larvicidal activity against Ae. aegypti and Ae. albopictus. The larvicidal potential of chloroform extract was further ascertained when its A7 fraction exhibited strong toxic effect against Ae. aegypti (LC50 = 4.7 \u00b5g\/mL) and Ae. albopictus (LC50 = 5.3 \u00b5g\/mL). LC-MS analysis of the chloroform extract gave a tentative identification of 13 compounds; Bis-(3-oxoundecyl) tetrasulfide was identified as the major compound in A7 fraction. Methanol extract showed strong repellent effect against female oviposition, along with weak adulticidal activity against mosquito and weak toxicity against brine shrimp nauplii. The mosquitocidal results of B. pennata suggest further investigation for the development of effective insecticide.<\/jats:p>","DOI":"10.3390\/molecules200814082","type":"journal-article","created":{"date-parts":[[2015,8,5]],"date-time":"2015-08-05T03:18:55Z","timestamp":1438744735000},"page":"14082-14102","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Mosquitocidal and Oviposition Repellent Activities of the Extracts of Seaweed Bryopsis pennata on Aedes aegypti and Aedes albopictus"],"prefix":"10.3390","volume":"20","author":[{"given":"Ke-Xin","family":"Yu","sequence":"first","affiliation":[{"name":"Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia,  50300 Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Lee","family":"Wong","sequence":"additional","affiliation":[{"name":"School of Biosciences, Taylor's University, Taylor's Lakeside Campus, Subang Jaya,  47500 Selangor, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohani","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Medical Entomology Unit, Infectious Disease Research Centre, Institute for Medical Research, 50588 Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"Jantan","sequence":"additional","affiliation":[{"name":"Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia,  50300 Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,8,4]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization Impact of Dengue. Available online: http:\/\/www.who.int\/csr\/disease\/dengue\/impact\/en\/."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"571","DOI":"10.2217\/fvl.09.55","article-title":"Global spread of epidemic dengue: The influence of environmental change","volume":"4","author":"Ooi","year":"2009","journal-title":"Future Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"200","DOI":"10.4269\/ajtmh.2011.10-0503","article-title":"Economic impact of dengue illness in the Americas","volume":"84","author":"Shepard","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_4","unstructured":"Ng, F.S.P., and Yong, H.S. (2000). Mosquitoes and Mosquito-Borne Diseases: Biology, Surveillance, Control, Personal and Public Protection Measures, Academy of Sciences Malaysia."},{"key":"ref_5","unstructured":"World Health Organization Guidelines for Dengue Surveillance and Mosquito Control. Available online: http:\/\/www.wpro.who.int\/publications\/pub_9290610689\/en\/."},{"key":"ref_6","unstructured":"Ng, F.S.P., and Yong, H.S. (2000). Mosquitoes and Mosquito-Borne Diseases: Biology, Surveillance, Control, Personal and Public Protection Measures, Academy of Sciences Malaysia."},{"key":"ref_7","first-page":"100","article-title":"Sri Lankan Seaweeds: Methodologies and field guide to the dominant species","volume":"Volume 6","author":"Samyn","year":"2009","journal-title":"Abc Taxa"},{"key":"ref_8","first-page":"73","article-title":"Antibacterial and antifungal activities of marine algae from Karachi seashore of Pakistan","volume":"55","author":"Usmanghani","year":"1984","journal-title":"Fitoterapia"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1007\/s00227-006-0376-3","article-title":"Antimicrobial activities of extracts from Indo-Pacific marine plants against marine pathogens and saprophytes","volume":"150","author":"Puglisi","year":"2007","journal-title":"Mar. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Freitas, J.C., Sakamoto, M.I., and Caprara, L. (1995). Cardiac effects induced by extract of the seaweed Bryopsis pennata (Chlorophyta, Caulerpales). Toxicon, 33.","DOI":"10.1016\/0041-0101(95)99356-8"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5825","DOI":"10.1021\/ja00066a061","article-title":"A bioactive depsipeptide from the sacoglossan mollusk Elysia rufescens and the green alga Bryopsis sp.","volume":"115","author":"Hamann","year":"1993","journal-title":"J. Am. Chem. Soc."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1854","DOI":"10.1158\/1078-0432.CCR-04-1534","article-title":"Phase I clinical and pharmacokinetic study of Kahalalide F in patients with advanced androgen refractory prostate cancer","volume":"11","author":"Horenblas","year":"2005","journal-title":"Clin. Cancer Res."},{"key":"ref_13","first-page":"271","article-title":"Effect of extract of the seaweed Bryopsis plumosa (Huds.) Ag. on the feeding rate and protein profile of haemolymph and fat body of Hyblaea puera (Cramer) (Lepidoptera: Hyblaeidae)","volume":"29","author":"Biju","year":"2014","journal-title":"Entomon"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1023\/B:JAPH.0000047783.36600.ef","article-title":"Medicinal and pharmaceutical uses of seaweed natural products: A review","volume":"16","author":"Smit","year":"2004","journal-title":"J. Appl. Phycol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/S2222-1808(13)60040-7","article-title":"Mosquito larvicidal activity of seaweeds extracts against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus","volume":"3","author":"Ali","year":"2013","journal-title":"Asian Pac. J. Trop. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"S143","DOI":"10.1016\/S2221-1691(11)60143-3","article-title":"Mosquito larvicidal efficacy of seaweed extracts against dengue vector of Aedes aegypti","volume":"1","author":"Beula","year":"2011","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.indcrop.2012.07.032","article-title":"Larvicidal activity of seaweeds from northeastern Brazil and of a halogenated sesquiterpene against the dengue mosquito Aedes aegypti","volume":"43","author":"Bianco","year":"2013","journal-title":"Ind. Crop. Prod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3121","DOI":"10.1007\/s00436-014-4068-5","article-title":"The major bioactive components of seaweeds and their mosquitocidal potential","volume":"113","author":"Yu","year":"2014","journal-title":"Parasitol. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1590\/S0074-02762003000400027","article-title":"Larvicidal activity of the essential oil from Lippia sidoides Cham. against Aedes aegypti Linn","volume":"98","author":"Carvalho","year":"2003","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S1226-8615(08)60270-8","article-title":"Impact analysis of neem kernel extracts on the developmental profile of Anopheles stephensi","volume":"9","author":"Sharma","year":"2006","journal-title":"J. Asia -Pac. Entomol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3275","DOI":"10.1007\/s00436-013-3506-0","article-title":"Larvicidal, oviposition, and ovicidal effects of Artemisia annua (Asterales: Asteraceae) against Aedes aegypti, Anopheles sinensis, and Culex quinquefasciatus (Diptera: Culicidae)","volume":"112","author":"Cheah","year":"2013","journal-title":"Parasitol. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1007\/s00436-014-3784-1","article-title":"Ovicidal, larvicidal and adulticidal properties of Asparagus racemosus (Willd.) (Family: Asparagaceae) root extracts against filariasis (Culex quinquefasciatus), dengue (Aedes aegypti) and malaria (Anopheles stephensi) vector mosquitoes (Diptera: Culicidae)","volume":"113","author":"Govindarajan","year":"2014","journal-title":"Parasitol. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1007\/s00436-012-3252-8","article-title":"Ovicidal, repellent, adulticidal and field evaluations of plant extract against dengue, malaria and filarial vectors","volume":"112","author":"Kovendan","year":"2013","journal-title":"Parasitol. Res."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Reegan, A.D., Kinsalin, A.V., Paulraj, M.G., and Ignacimuthu, S. (2013). Larvicidal, ovicidal, and repellent activities of marine sponge Cliona celata (Grant) extracts against Culex quinquefasciatus Say and Aedes aegypti L. (Diptera: Culicidae). ISRN Entomol., 2013.","DOI":"10.1155\/2013\/315389"},{"key":"ref_25","first-page":"141","article-title":"Combined effect of seaweed (Sargassum wightii) and Bacillus thuringiensis var. israelensis on the coastal mosquito, Anopheles sundaicus","volume":"38","author":"Kumar","year":"2012","journal-title":"Tamil Nadu India Sci. Asia"},{"key":"ref_26","unstructured":"Wildey, K.B. (1996, January 7\u201310). Marine Plants for Mosquito Control. Proceedings of the Second International Conference on Urban Pests, Edinburgh, Scotland, UK."},{"key":"ref_27","first-page":"61","article-title":"Adulticidal activity of some Malaysian plant extracts against Aedes aegypti Linnaeus","volume":"21","author":"Hidayatulfathi","year":"2004","journal-title":"Trop. Biomed."},{"key":"ref_28","first-page":"434","article-title":"Adulticidal activity of essential oil of Lantana camara leaves against mosquitoes","volume":"131","author":"Dua","year":"2010","journal-title":"Indian J. Med. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1146\/annurev.ento.51.110104.151146","article-title":"Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world","volume":"51","author":"Isman","year":"2006","journal-title":"Annu. Rev. Entomol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1007\/s00436-011-2669-9","article-title":"Adulticidal and repellent properties of indigenous plant extracts against Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae)","volume":"110","author":"Govindarajan","year":"2012","journal-title":"Parasitol. Res."},{"key":"ref_31","first-page":"340","article-title":"Potential of crude seed extract of celery, Apium graveolens L., against the mosquito Aedes aegypti (L.) (Diptera: Culicidae)","volume":"29","author":"Choochote","year":"2004","journal-title":"J. Vector Ecol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"272","DOI":"10.3923\/ijzr.2011.272.278","article-title":"In vitro mosquito larvicidal activity of marine algae against the human vectors, Culex quinquefasciatus (Say) and Aedes aegypti (Linnaeus) (Diptera: Culicidae)","volume":"7","author":"Manilal","year":"2011","journal-title":"Int. J. Zool. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1007\/s00436-013-3687-6","article-title":"Essential oils and their compounds as Aedes aegypti L. (Diptera: Culicidae) larvicides: Review","volume":"113","author":"Dias","year":"2014","journal-title":"Parasitol. Res."},{"key":"ref_34","first-page":"581","article-title":"Plant extracts as potential mosquito larvicides","volume":"135","author":"Ghosh","year":"2012","journal-title":"Indian J. Med. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1007\/s00436-007-0864-5","article-title":"Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet","volume":"102","author":"Rahuman","year":"2008","journal-title":"Parasitol. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0960-8524(96)00157-5","article-title":"Indigenous-plant extracts as larvicidal agents against Culex quinquefasciatus Say","volume":"59","author":"Karmegam","year":"1997","journal-title":"Bioresour. Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1002\/ps.3331","article-title":"Structure\u2013activity relationships of eugenol derivatives against Aedes aegypti (Diptera: Culicidae) larvae","volume":"68","author":"Barbosa","year":"2012","journal-title":"Pest Manag. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/(SICI)1099-1573(199703)11:2<145::AID-PTR40>3.0.CO;2-#","article-title":"Antivectorial activities of cashew nut shell extracts from Anacardium occidentale L.","volume":"11","author":"Laurens","year":"1997","journal-title":"Phytother. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/BF00987923","article-title":"Response of Aedes triseriatus larvae to fatty acids of Cladophora","volume":"5","author":"LaLonde","year":"1979","journal-title":"J. Chem. Ecol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1002\/clen.201000033","article-title":"Insecticidal metabolites from the green alga Caulerpa racemosa","volume":"38","author":"Alarif","year":"2010","journal-title":"Clean Soil Air Water"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Moore, R.E. (1971). Bis-(3-oxoundecyl) polysulphides in Dictyopteris. J. Chem. Soc. D Chem. Commun., 1168b\u20131169.","DOI":"10.1039\/c2971001168b"},{"key":"ref_42","first-page":"433","article-title":"Marine algal natural products: Contributions from India in the global context","volume":"45B","author":"Sarma","year":"2006","journal-title":"Indian J. Chem. Sec. B"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"138","DOI":"10.3376\/1081-1710(2006)31[138:LEOPPO]2.0.CO;2","article-title":"Larvicidal effect of pepper plants on Aedes aegypti (L.) (Diptera: Culicidae)","volume":"31","author":"Chaithong","year":"2006","journal-title":"J. Vector Ecol."},{"key":"ref_44","first-page":"195","article-title":"Possible site of action of Kaempferia galanga in killing Culex quinquefasciatus larvae","volume":"30","author":"Insun","year":"1999","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_45","first-page":"282","article-title":"Larvicidal activity of Tagetes minuta (Marigold) toward Aedes aegypti","volume":"7","author":"Green","year":"1991","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_46","unstructured":"Christophers, S.R. (1960). Aedes a\u00ebgypti (L.) the Yellow Fever Mosquito: Its Life History, Bionomics and Structure, Cambridge University Press."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1248\/cpb.57.819","article-title":"Improved microwave-mediated synthesis of 3-(3-aryl-1,2,4-oxadiazol-5-yl) propionic acids and their larvicidal and fungal growth inhibitory properties","volume":"57","author":"Brustein","year":"2009","journal-title":"Chem. Pharm. Bull."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3477","DOI":"10.1007\/s00436-013-3528-7","article-title":"Impact of Argemone mexicana extracts on the cidal, morphological, and behavioral response of dengue vector, Aedes aegypti L. (Diptera: Culicidae)","volume":"112","author":"Warikoo","year":"2013","journal-title":"Parasitol. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0048-3575(90)90134-N","article-title":"Two insecticidal monoterpenes, telfairine and aplysiaterpenoid A, from the red alga Plocamium telfairiae: Structure elucidation, biological activity, and molecular topographical consideration by a semiempirical molecular orbital study","volume":"37","author":"Watanabe","year":"1990","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1177\/009286159803200223","article-title":"The use of biological assays to evaluate botanicals","volume":"32","author":"Mclaughlin","year":"1998","journal-title":"Drug Info. J."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Guedes, E.A.C., de Carvalho, C.M., Ribeiro Junior, K.A.L., Lisboa Ribeiro, T.F., de Barros, L.D., de Lima, M.R.F., Prado Moura, F.B., and Goulart Sant\u2019Ana, A.E. (2014). Larvicidal activity against Aedes aegypti and molluscicidal activity against Biomphalaria glabrata of Brazilian marine algae. J. Parasitol. Res., 2014.","DOI":"10.1155\/2014\/501328"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2490","DOI":"10.3923\/jas.2009.2490.2493","article-title":"In vitro antioxidant activity of the hexane and methanolic extracts of Sargassum baccularia and Cladophora patentiramea","volume":"9","author":"Sheikh","year":"2009","journal-title":"J. Appl. Sci."},{"key":"ref_53","first-page":"323","article-title":"Extraction of plant secondary metabolites","volume":"Volume 20","author":"Sarker","year":"2005","journal-title":"Natural Products Isolation"},{"key":"ref_54","unstructured":"World Health Organization Guidelines for Laboratory and Field Testing of Mosquito Larvicides. Available online: http:\/\/www.who.int\/whopes\/guidelines\/en\/."},{"key":"ref_55","first-page":"412","article-title":"Delayed mortality and morphogenetic anomalies induced in Culex quinquefasciatus by the microbial control agent Bacillus sphaericus","volume":"7","author":"Mulla","year":"1991","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_56","unstructured":"World Health Organization Report of the WHO Informal Consultation. Test Procedures for Insecticide Resistance Monitoring in Malaria Vectors, Bioefficacy and Persistence of Insecticides on Treated Surfaces. Available online: http:\/\/www.who.int\/whopes\/resistance\/en\/."},{"key":"ref_57","first-page":"15","article-title":"Oviposition deterrent and skin repellent activities of Solanum trilobatum leaf extract against the malarial vector Anopheles stephensi","volume":"5","author":"Rajkumar","year":"2005","journal-title":"J. Insect Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1093\/ee\/8.6.1111","article-title":"Oviposition attractants and repellents of mosquitoes: Oviposition responses of Culex mosquitoes to organic infusions","volume":"8","author":"Kramer","year":"1979","journal-title":"Environ. Entomol."},{"key":"ref_59","unstructured":"Buckingham, J. Dictionary of Natural Products. Available online: http:\/\/dnp.chemnetbase.com\/dictionary-search.do?method=view&id=11136608&si=."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Blunt, J., and Munro, M.H. (2008). Dictionary of Marine Natural Products with Cd-Rom, Chapman and Hall\/CRC.","DOI":"10.1201\/9780849382178"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2282","DOI":"10.1007\/s11434-013-5745-y","article-title":"Bioactivity and constituents of several common seaweeds","volume":"58","author":"Zhang","year":"2013","journal-title":"Chin. Sci. Bull."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1093\/jee\/18.2.265a","article-title":"A method of computing the effectiveness of an insecticide","volume":"18","author":"Abbots","year":"1925","journal-title":"J. Econ. Entomol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/20\/8\/14082\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:50:10Z","timestamp":1760215810000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/20\/8\/14082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,4]]},"references-count":62,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2015,8]]}},"alternative-id":["molecules200814082"],"URL":"https:\/\/doi.org\/10.3390\/molecules200814082","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,4]]}}}