{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:37:56Z","timestamp":1784043476060,"version":"3.55.0"},"reference-count":20,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Phytochemical studies on the constituents of the rhizomes of Imperata cylindrica (Gramineae) were performed using high-performance liquid chromatography (HPLC). We also aimed to search for any biologically active substance capable of inhibiting nitric oxide (NO) formation in lipopolysaccharide (LPS)-activated macrophage 264.7 cells, by testing four compounds isolated from this plant. Four compounds, including a new chromone, isoeugenin, along with ferulic acid, p-coumaric acid, and caffeic acid were isolated and identified by NMR spectroscopy. The structure of isoeugenin was determined as 7-hydroxy-5-methoxy-2-methylchromone by the 2D-NMR technique. Among the four compounds, isoeugenin has the lowest IC50 value on the inhibition of NO production in LPS-activated macrophage RAW264.7 cells (IC50, 9.33 \u03bcg\/mL). In addition, isoeugenin significantly suppressed the LPS-induced expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory cytokines mRNA levels. Taken together, these results suggest that the anti-inflammatory activity of isoeugenin is associated with the down-regulation of iNOS, COX-2, and pro-inflammatory cytokines in RAW264.7 cells. Accordingly, our results suggest that the new chromone isoegenin should be considered a potential treatment for inflammatory disease.<\/jats:p>","DOI":"10.3390\/molecules201219767","type":"journal-article","created":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T10:26:58Z","timestamp":1448965618000},"page":"21336-21345","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Isoeugenin, a Novel Nitric Oxide Synthase Inhibitor Isolated from the Rhizomes of Imperata cylindrica"],"prefix":"10.3390","volume":"20","author":[{"given":"Hyo-Jin","family":"An","sequence":"first","affiliation":[{"name":"Department of Pharmacology, College of Korean Medicine, Sangji University, Wonju 220-702, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7236-9654","authenticated-orcid":false,"given":"Agung","family":"Nugroho","sequence":"additional","affiliation":[{"name":"Department of Agro-Industrial Technology, Faculty of Agriculture, Lambung Mangkurat University, Indonesia 70712, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byong-Min","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Forest Science, College of Life Science and Natural Resources, Sangji University, Wonju 220-702, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee-Juhn","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Engineering, College of Health Science, Sangji University, Wonju 220-702, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.6116\/kjh.2014.29.6.27.","article-title":"Effects of Imperatae Rhizoma Extract on T helper 2 cell differentiation","volume":"29","author":"Kim","year":"2014","journal-title":"Korean J. 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