{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:45:59Z","timestamp":1762145159895,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2015,12,3]],"date-time":"2015-12-03T00:00:00Z","timestamp":1449100800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Allergic contact dermatitis (ACD) remains a major skin disease in many countries, necessitating the discovery of novel and effective anti-ACD agents. In this study, we investigated the preventive effects of Achyranthis radix extract (AcRE) on trimellitic anhydride (TMA)-induced dermatitis and the potential mechanism of action involved. Oral administration of AcRE and prednisolone (PS) significantly suppressed TMA-induced increases in ear and epidermal thickness, and IgE expression. In addition, abnormal expression of IL-1\u03b2 and TNF-\u03b1 protein and mRNA was also significantly attenuated by oral administration of AcRE. Treatment with AcRE also significantly suppressed TMA-induced IL-4 and IL-13 cytokines and mRNA expression in vivo. Moreover, AcRE strongly suppressed TMA-induced IL-4 and IL-5 production in draining lymph nodes, as well as OVA-induced IL-4 and IL-5 expression in primary cultured splenocytes. Interestingly, AcRE suppressed IL-4-induced STAT6 phosphorylation in both primary cultured splenocytes and HaCaT cells, and TMA-induced GATA3 mRNA expression ex vivo. AcRE also suppressed TMA-mediated CCL11 and IL-4-induced CCL26 mRNA expression and infiltration of CCR3 positive cells. The major compounds from AcRE were identified as gentisic acid (0.64 \u00b1 0.2 \u03bcg\/g dry weight of AcRE), protocatechuic acid (2.69 \u00b1 0.1 \u03bcg\/g dry weight of AcRE), 4-hydroxybenzoic acid (5.59 \u00b1 0.3 \u03bcg\/g dry weight of AcRE), caffeic acid (4.21 \u00b1 0.1 \u03bcg\/g dry weight of AcRE), and ferulic acid (14.78 \u00b1 0.4 \u00b1 0.3 \u03bcg\/g dry weight of AcRE). Taken together, these results suggest that AcRE has potential for development as an agent to prevent and treat allergic contact dermatitis.<\/jats:p>","DOI":"10.3390\/molecules201219788","type":"journal-article","created":{"date-parts":[[2015,12,3]],"date-time":"2015-12-03T11:12:09Z","timestamp":1449141129000},"page":"21584-21596","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Oral Administration of Achyranthis radix Extract Prevents TMA-induced Allergic Contact Dermatitis by Regulating Th2 Cytokine and Chemokine Production in Vivo"],"prefix":"10.3390","volume":"20","author":[{"given":"Sung","family":"Jung","sequence":"first","affiliation":[{"name":"Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea"},{"name":"Food Biotechnology Program, Korea University of Science and Technology, Daejeon 34113, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dae","family":"Choi","sequence":"additional","affiliation":[{"name":"Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea"},{"name":"Food Biotechnology Program, Korea University of Science and Technology, Daejeon 34113, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da-Ae","family":"Kwon","sequence":"additional","affiliation":[{"name":"Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Kim","sequence":"additional","affiliation":[{"name":"Research Group of Metabolic Mechanism, Korea Food Research Institute, Seongnam 13539, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ki","family":"Seong","sequence":"additional","affiliation":[{"name":"Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Hwa","family":"Shon","sequence":"additional","affiliation":[{"name":"Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam 13539, Korea"},{"name":"Food Biotechnology Program, Korea University of Science and Technology, Daejeon 34113, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2015,12,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s00018-011-0846-8","article-title":"Allergic contact dermatitis: Epidemiology, molecular mechanisms, in vitro methods and regulatory aspects. 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