{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:30:56Z","timestamp":1790026256300,"version":"4.0.1"},"reference-count":56,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T00:00:00Z","timestamp":1452470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Dugesia tigrina is a non-parasitic platyhelminth, which has been recently utilized in pharmacological models, regarding the nervous system, as it presents a wide sensitivity to drugs. Our trials aimed to propose a model for an in vivo screening of substances with inhibitory activity of the enzyme acetylcholinesterase. Trials were performed with four drugs commercialized in Brazil: donepezil, tacrine, galantamine and rivastigmine, utilized in the control of Alzheimer\u2019s disease, to inhibit the activity of acetylcholinesterase. We tested five concentrations of the drugs, with an exposure of 24 h, and the mortality and the inhibition of acetylcholinesterase planarian seizure-like activity (pSLA) and planarian locomotor velocity (pLMV) were measured. Galantamine showed high anticholinesterasic activity when compared to the other drugs, with a reduction of 0.05 \u03bcmol\u00b7min\u22121 and 63% of convulsant activity, presenting screw-like movement and hypokinesia, with pLMV of 65 crossed lines during 5 min. Our results showed for the first time the anticholinesterasic and convulsant effect, in addition to the decrease in locomotion induced by those drugs in a model of invertebrates. The experimental model proposed is simple and low cost and could be utilized in the screening of substances with anticholinesterasic action.<\/jats:p>","DOI":"10.3390\/molecules21010053","type":"journal-article","created":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T10:09:38Z","timestamp":1452506978000},"page":"53","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Effect of Donepezil, Tacrine, Galantamine and Rivastigmine on Acetylcholinesterase Inhibition in Dugesia tigrina"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5067-4781","authenticated-orcid":false,"given":"Cristiane","family":"Bezerra da Silva","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Federal University of Paran\u00e1 (UFPR), Av. Pref. Loth\u00e1rio Meissner 3400, Jardim Bot\u00e2nico, Curitiba 80210-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnildo","family":"Pott","sequence":"additional","affiliation":[{"name":"Department of Biology, Federal University of Mato Grosso do Sul (UFMS), Av. Senador Filinto M\u00fcller, Campo Grande 79046-460, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selene","family":"Elifio-Esposito","sequence":"additional","affiliation":[{"name":"Post-Graduation in Health Sciences, Pont\u00edficia Universidade Cat\u00f3lica do Paran\u00e1, Imaculada Concei\u00e7\u00e3o, 1155, Prado Velho, Curitiba 80215-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciane","family":"Dalarmi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Paran\u00e1 (UFPR), Av. Pref. Loth\u00e1rio Meissner 3400, Jardim Bot\u00e2nico, Curitiba 80210-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e1tia","family":"Fialho do Nascimento","sequence":"additional","affiliation":[{"name":"Department of Celular Biology, Federal University of Paran\u00e1 (UFPR), Centro Polit\u00e9cnico, Jardim das Am\u00e9ricas, Curitiba 81530-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ligia","family":"Moura Burci","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Paran\u00e1 (UFPR), Av. Pref. Loth\u00e1rio Meissner 3400, Jardim Bot\u00e2nico, Curitiba 80210-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maislian","family":"De Oliveira","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Paran\u00e1 (UFPR), Av. Pref. Loth\u00e1rio Meissner 3400, Jardim Bot\u00e2nico, Curitiba 80210-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josiane","family":"De F\u00e1tima Gaspari Dias","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Paran\u00e1 (UFPR), Av. Pref. Loth\u00e1rio Meissner 3400, Jardim Bot\u00e2nico, Curitiba 80210-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Maria Warumby Zanin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Paran\u00e1 (UFPR), Av. Pref. Loth\u00e1rio Meissner 3400, Jardim Bot\u00e2nico, Curitiba 80210-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Obdulio","family":"Gomes Miguel","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Paran\u00e1 (UFPR), Av. Pref. Loth\u00e1rio Meissner 3400, Jardim Bot\u00e2nico, Curitiba 80210-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilis","family":"Dallarmi Miguel","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Federal University of Paran\u00e1 (UFPR), Av. Pref. Loth\u00e1rio Meissner 3400, Jardim Bot\u00e2nico, Curitiba 80210-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,1,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/S0165-0173(97)00016-7","article-title":"Cholinergic activity and amyloid precursor protein metabolism","volume":"25","author":"Roberson","year":"1997","journal-title":"Brain Res. 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