{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:06:31Z","timestamp":1790431591335,"version":"4.1.0"},"reference-count":38,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2016,1,9]],"date-time":"2016-01-09T00:00:00Z","timestamp":1452297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Alisma canaliculatum is a herb commonly used in traditional Korean medicine, and has been shown in scientific studies to have antitumor, diuretic hepatoprotective, and antibacterial effects. Recently, the anti-osteoclastogenesis of alisol A 24-acetate from Alisma canaliculatum was investigated in vitro. However, the influence of alisol A 24-acetate on osteoporosis in animals has not been investigated. The present study was undertaken to investigate the anti-osteoporotic effect of alisol A 24-acetate on bone mass in ovariectomized (OVX) mice and to identify the mechanism responsible for its effects. OVX mice were treated daily with 0.5 or 2 \u03bcg\/g of alisol A 24-acetate for a period of six weeks. It was found that these administrations significantly suppressed osteoporosis in OVX mice and improved bone morphometric parameters. The serum estradiol, bone alkaline phosphatase levels, regulatory T\/Th17 cell numbers were significantly increased by alisol A 24-acetate as compared with untreated OVX mice. In addition, TRAP activity was inhibited by alisol A 24-acetate in OVX mice. These results suggest alisol A 24-acetate effectively prevents bone loss in OVX mice, and that it can be considered a potential therapeutic for the treatment of postmenopausal osteoporosis.<\/jats:p>","DOI":"10.3390\/molecules21010074","type":"journal-article","created":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T10:09:38Z","timestamp":1452506978000},"page":"74","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The Protective Effects of Alisol A 24-Acetate from Alisma canaliculatum on Ovariectomy Induced Bone Loss in Vivo"],"prefix":"10.3390","volume":"21","author":[{"given":"Yun-Ho","family":"Hwang","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Yun","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Ju","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Jip","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Jin","family":"Son","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Tae","family":"Yee","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"25103","DOI":"10.1074\/jbc.R109.041087","article-title":"Cellular and molecular mechanisms of bone remodeling","volume":"285","author":"Raggatt","year":"2010","journal-title":"J. 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