{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T14:32:28Z","timestamp":1785335548986,"version":"3.55.0"},"reference-count":37,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2016,4,19]],"date-time":"2016-04-19T00:00:00Z","timestamp":1461024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Crude extracts of Sedum formosanum N.E.Br. obtained from n-butanol partition (BP) and isopropanol salting-out pretreatment (ISP) were analyzed using antioxidation assays. The results indicated that the extract from ISP contained more potent antioxidants and thus exhibited more antioxidant activity in all the assays. The superoxide radical-scavenging activity and inhibition of nitric oxide radicals achieved after ISP were 3.65 and 2.18 times higher than those achieved through BP, respectively. Eight bioactive natural products were isolated and identified according to an analysis of antioxidation activity in different fractions of the ISP crude extract, namely three cyanophoric glycosides 1\u20133, three flavonoids 4\u20136 and two phenolic compounds (7 and a new compound 8). Among them, compounds 5 and 6 exhibit the highest antioxidation capability, and the ISP is suitable for obtaining compounds 5 and 6 using HPLC chromatograms. Therefore, ISP is an excellent extraction technology that can be used to extract antioxidant compounds in the nutraceutical and pharmaceutical industries.<\/jats:p>","DOI":"10.3390\/molecules21040513","type":"journal-article","created":{"date-parts":[[2016,4,19]],"date-time":"2016-04-19T10:41:58Z","timestamp":1461062518000},"page":"513","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparison of Antioxidant Capability after Isopropanol Salting-Out Pretreatment and n-Butanol Partition Extraction, and Identification and Evaluation of Antioxidants of Sedum formosanum N.E.Br."],"prefix":"10.3390","volume":"21","author":[{"given":"Jung-Hui","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering, Nan-Kai University of Technology, 568 ZhongZheng Road, Caotun, Nantou County 542, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Hui","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Chung Hsing University 250 Kuo-Kuang Road, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang-Dung","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Chung Hsing University 250 Kuo-Kuang Road, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Fong","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Chung Hsing University 250 Kuo-Kuang Road, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih-Lan","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Taichung Veterans General Hospital, 1650 Taiwan Road Sec, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Yuan","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Chung Hsing University 250 Kuo-Kuang Road, Taichung 402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,4,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0304-4165(03)00008-4","article-title":"ESR study of a biological assay on whole blood: Antioxidant efficiency of various vitamins","volume":"1621","author":"Stocker","year":"2003","journal-title":"Biochem. 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