{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T00:29:19Z","timestamp":1776472159885,"version":"3.51.2"},"reference-count":28,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2016,8,16]],"date-time":"2016-08-16T00:00:00Z","timestamp":1471305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Chlorophyll-type contaminants are commonly encountered in the isolation and determination of flavones of plant aerial plant parts. Heme is also a difficult background substance in whole blood analysis. Both chlorophyll and heme are porphyrin type compounds. In this study, a rapid method for isolating flavones with 5-hydroxyl or ortho-hydroxyl groups from biological samples was developed based on the different solubilities of porphyrin-metal and flavone-metal complexes. It is important that other background substances, e.g., proteins and lipids, are also removed from flavones without an additional processing. The recoveries of scutellarin, baicalin, baicalein, wogonoside and wogonin, which are the primary constituents of Scutellaria baicalensis (skullcaps) were 99.65% \u00b1 1.02%, 98.98% \u00b1 0.73%, 99.65% \u00b1 0.03%, 97.59% \u00b1 0.09% and 95.19% \u00b1 0.47%, respectively. As a sample pretreatment procedure, this method was coupled to high-performance liquid chromatography (HPLC) with good separation, sensitivity and linearity and was applied to determine the flavone content in different aerial parts of S. baicalensis and in dried blood spot samples.<\/jats:p>","DOI":"10.3390\/molecules21081067","type":"journal-article","created":{"date-parts":[[2016,8,16]],"date-time":"2016-08-16T10:03:21Z","timestamp":1471341801000},"page":"1067","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Rapid Isolation and Determination of Flavones in Biological Samples Using Zinc Complexation Coupled with High-Performance Liquid Chromatography"],"prefix":"10.3390","volume":"21","author":[{"given":"Chenghe","family":"Sun","sequence":"first","affiliation":[{"name":"Institute of Special Wild Animals and Plants, Chinese Academy of Agricultural Science, Changchun 130122, China"},{"name":"School of Life Science, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hecheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Special Wild Animals and Plants, Chinese Academy of Agricultural Science, Changchun 130122, China"},{"name":"School of Life Science, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingping","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Special Wild Animals and Plants, Chinese Academy of Agricultural Science, Changchun 130122, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0039-8325","authenticated-orcid":false,"given":"Shengyuan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Institute of Special Wild Animals and Plants, Chinese Academy of Agricultural Science, Changchun 130122, China"},{"name":"School of Life Science, Beijing Institute of Technology, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,8,16]]},"reference":[{"key":"ref_1","first-page":"2861","article-title":"Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin","volume":"20","author":"Shieh","year":"2000","journal-title":"Anticancer Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/S0090-4295(00)00467-2","article-title":"Antitumor effects of Scutellariae radix and its components baicalein, baicalin, and wogonin on bladder cancer cell lines","volume":"55","author":"Ikemoto","year":"2000","journal-title":"Urology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.ctrv.2008.09.005","article-title":"New therapeutic aspects of flavones: The anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin","volume":"35","year":"2009","journal-title":"Cancer Treat. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1006\/bbrc.2000.3485","article-title":"Flavonoid Baicalin Inhibits HIV-1 Infection at the Level of Viral Entry","volume":"276","author":"Li","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Yuan, Y., Rangarajan, P., Kan, E.M., Wu, Y., Wu, C., and Ling, E.-A. (2015). Scutellarin regulates the Notch pathway and affects the migration and morphological transformation of activated microglia in experimentally induced cerebral ischemia in rats and in activated BV-2 microglia. J. Neuroinflamm., 12.","DOI":"10.1186\/s12974-014-0226-z"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.jep.2015.02.031","article-title":"Scutellarin protects against the liver injury induced by diosbulbin B in mice and its mechanism","volume":"164","author":"Niu","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1055\/s-2008-1034358","article-title":"Comparisons of Scutellaria baicalensis, Scutellaria lateriflora and Scutellaria racemosa: genome size, antioxidant potential and phytochemistry","volume":"74","author":"Cole","year":"2008","journal-title":"Plant. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.phymed.2008.07.011","article-title":"Characterization of chemical ingredients and anticonvulsant activity of American skullcap (Scutellaria lateriflora)","volume":"16","author":"Zhang","year":"2009","journal-title":"Phytomedicine"},{"key":"ref_9","first-page":"471","article-title":"Chemical constituents of the aerial parts of Scutellaria lateriflora and their alpha-glucosidase inhibitory activities","volume":"7","author":"Kuroda","year":"2012","journal-title":"Nat. Prod. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1097\/FJC.0000000000000109","article-title":"Scutellarin Inhibits High Glucose\u2013induced and Hypoxia-mimetic Agent\u2013induced Angiogenic Effects in Human Retinal Endothelial Cells through Reactive Oxygen Species\/Hypoxia-inducible Factor-1\u03b1\/Vascular Endothelial Growth Factor Pathway","volume":"64","author":"Wang","year":"2014","journal-title":"J. Cardiovasc. Pharmacol."},{"key":"ref_11","first-page":"89","article-title":"Determination of baicalin in the leaf of Scutellaria baicalensis with HPLC","volume":"23","author":"Chang","year":"2009","journal-title":"Nei. Mongol. J. Tradit. Chin. Med."},{"key":"ref_12","unstructured":"Pryor, J.N., Bogdanor, J.M., and Welsh, W.A. (1988). Process for the Removal of Chlorophyll, Color Bodies and Phospholipids from Glyceride oils Using Acid-Treated Silica Adsorbents. (4781864 A), U.S. Patent."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.aca.2008.03.031","article-title":"A multi-residue method for the determination of 203 pesticides in rice paddies using gas chromatography\/mass spectrometry","volume":"619","author":"Nguyen","year":"2008","journal-title":"Anal. Chim. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Hostettmann, K., Marston, A., and Hostettmann, M. (1998). Preparative Chromatography Techniques: Applications in Nat. Product Isolation, Springer. [2nd ed.].","DOI":"10.1007\/978-3-662-03631-0"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.aca.2011.07.018","article-title":"Pressurized liquid extraction as a green approach in food and herbal plants extraction: A review","volume":"703","author":"Mustafa","year":"2011","journal-title":"Anal. Chim. Acta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.foodchem.2014.04.098","article-title":"Tackling the challenge of selective analytical clean-up of complex Nat. extracts: The curious case of chlorophyll removal","volume":"163","author":"Bijttebier","year":"2014","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/bmc.1367","article-title":"Dried blood spot sampling in combination with LC\/MS\/MS for quantitative analysis of small molecules","volume":"24","author":"Li","year":"2010","journal-title":"Biomed. Chromatogr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1002\/jcph.170","article-title":"Estimation and comparison of carbamazepine population pharmacokinetics using dried blood spot and plasma concentrations from people with epilepsy: The clinical implication","volume":"54","author":"Kong","year":"2014","journal-title":"J. Clin. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1021\/ac403672u","article-title":"Fully-automated approach for online dried blood spot extraction and bioanalysis by two-dimensional-liquid chromatography coupled with high-resolution quadrupole time-of-flight mass spectrometry","volume":"86","author":"Oliveira","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Cao, J., Wang, Y., and Xiao, S. (2013). Simultaneous Determination of Glycyrrhizin and 15 Flavonoids in Licorice and Blood by High Performance Liquid Chromatography with Ultraviolet Detector. ISRN Anal. Chem., 2013.","DOI":"10.1155\/2013\/786151"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.chroma.2013.09.052","article-title":"Depletion of high-abundance flavonoids by metal complexation and identification of low-abundance flavonoids in Scutellaria baicalensis Georgi","volume":"1315","author":"Wang","year":"2013","journal-title":"J. Chromatogr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"12494","DOI":"10.1021\/jp064362q","article-title":"Spectroscopic and theoretical studies of the Zn(II) chelation with hydroxyflavones","volume":"110","author":"Lapouge","year":"2006","journal-title":"J. Phys. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/S0891-5849(01)00638-4","article-title":"The influence of pH on antioxidant properties and the mechanism of antioxidant action of hydroxyflavones","volume":"31","author":"Lemanska","year":"2001","journal-title":"Free Radical Biol. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.ab.2008.09.029","article-title":"A fluorometric high-performance liquid chromatography procedure for simultaneous determination of methylamine and aminoacetone in blood and tissues","volume":"384","author":"Xiao","year":"2009","journal-title":"Anal. Biochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1002\/pca.727","article-title":"Approach to the study of C-glycosyl flavones by ion trap HPLC-PAD-ESI\/MS\/MS: application to seeds of quince (Cydonia oblonga)","volume":"14","author":"Ferreres","year":"2003","journal-title":"Phytochem. Anal."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Islam, M.N., Downey, F., and Ng, C.K.Y. (2011). Comparative analysis of bioactive phytochemicals from Scutellaria baicalensis, Scutellaria lateriflora, Scutellaria racemosa, Scutellaria tomentosa and Scutellaria wrightii by LC-DAD-MS. Metabolomics, 446\u2013453.","DOI":"10.1007\/s11306-010-0269-9"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1080\/14786419.2011.631134","article-title":"A simple isocratic HPLC method for the simultaneous determination of bioactive components of Scutellariae radix extract","volume":"26","author":"Islam","year":"2012","journal-title":"Nat. Prod. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1007\/s11418-008-0230-7","article-title":"Comparison of the major flavonoid content of S. baicalensis; S. lateriflora; and their commercial products","volume":"62","author":"Makino","year":"2008","journal-title":"J. Nat. Med."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/21\/8\/1067\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:28:32Z","timestamp":1760210912000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/21\/8\/1067"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,16]]},"references-count":28,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2016,8]]}},"alternative-id":["molecules21081067"],"URL":"https:\/\/doi.org\/10.3390\/molecules21081067","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,16]]}}}