{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:50:58Z","timestamp":1790884258918,"version":"4.1.0"},"reference-count":27,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2016,12,27]],"date-time":"2016-12-27T00:00:00Z","timestamp":1482796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573563"],"award-info":[{"award-number":["81573563"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81274168"],"award-info":[{"award-number":["81274168"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The goal of this research was to evaluate the anti-hepatitis B virus (HBV) activities of three compounds extracted and purified from Herpetospermum seeds (HS) on HepG2.2.15 cells. Herpetin (HPT), herpetone (HPO), and herpetfluorenone (HPF) were isolated from HS and identified using HR-ESI-MS and NMR. Different concentrations of the drugs were added to the HepG2.2.15 cells. Cell toxicity was observed with an MTT assay, cell culture supernatants were collected, and HBsAg and HBeAg were detected by ELISA. The content of HBV DNA was determined via quantitative polymerase chain reaction (PCR) with fluorescent probes. The 50% toxicity concentration (TC50) of HPF was 531.48 \u03bcg\/mL, suggesting that this species is less toxic than HPT and HPO. HPT and HPF showed more potent antiviral activities than HPO. The 50% inhibition concentration (IC50) values of HPF on HBsAg and HBeAg were 176.99 and 134.53 \u03bcg\/mL, respectively, and the corresponding therapeutic index (TI) values were 2.66 and 3.49, respectively. HPT and HPF were shown to significantly reduce the level of HBV DNA in the HepG2.2.15 culture medium compared to the negative control. This initial investigation of the anti-HBV constituents of HS yielded three compounds that revealed a synergistic effect of multiple components in the ethnopharmacological use of HS.<\/jats:p>","DOI":"10.3390\/molecules22010014","type":"journal-article","created":{"date-parts":[[2016,12,28]],"date-time":"2016-12-28T11:22:14Z","timestamp":1482924134000},"page":"14","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Anti-HBV Activities of Three Compounds Extracted and Purified from Herpetospermum Seeds"],"prefix":"10.3390","volume":"22","author":[{"given":"Pu-Yang","family":"Gong","sequence":"first","affiliation":[{"name":"College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7809-2580","authenticated-orcid":false,"given":"Zhi-Xiang","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Gu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Medicine, Southwest Jiaotong University, Chengdu 610031, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Chuan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Medicine, Southwest Jiaotong University, Chengdu 610031, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,12,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.virol.2014.12.001","article-title":"Suppression of hepatitis B virus DNA accumulation in chronically infected cells using a bacterial CRISPR\/Cas RNA-guided DNA endonuclease","volume":"476","author":"Kennedy","year":"2015","journal-title":"Virology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1136\/pgmj.2006.047431","article-title":"Chronic hepatitis B in hepatocarcinogenesis","volume":"82","author":"Park","year":"2006","journal-title":"Postgrad. 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