{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:46:08Z","timestamp":1787312768652,"version":"build-2736575974"},"reference-count":56,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,1,2]],"date-time":"2017-01-02T00:00:00Z","timestamp":1483315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"RFBR; Government of Republic of Buryatia","award":["16-43-030857"],"award-info":[{"award-number":["16-43-030857"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Naturally existing \u03b1-glucosidase inhibitors from traditional herbal medicines have attracted considerable interest to treat type 2 diabetes mellitus (DM). The present study aimed to evaluate the anti-\u03b1-glucosidase activity of extracts from marsh cinquefoil (Comarum palustre L.), their hypoglycaemic action and detection of the responsible compounds. A 60% ethanol extract from C. palustre herb revealed the highest inhibitory activity against \u03b1-glucosidase (IC50 52.0 \u03bcg\/mL). The HPLC analysis of the major compounds resulted in detection of 15 compounds, including ellagitannins, flavonoids, catechin and other compounds. Using HPLC activity-based profiling a good inhibitory activity of agrimoniin-containing eluates against \u03b1-glucosidase was demonstrated. The removal of ellagitannins from the C. palustre extract significantly decreased \u03b1-glucosidase inhibition (IC50 204.7 \u03bcg\/mL) due to the high enzyme-inhibiting activity of the dominant agrimoniin (IC50 21.8 \u03bcg\/mL). The hypoglycaemic effect of C. palustre extracts before and after ellagitannin removal, agrimoniin and insulin was evaluated on streptozotocin-induced experimental model. Diabetic rats treated with agrimoniin and C. palustre extract before ellagitannin removal showed significant increases in the levels of plasma glucose and glycosylated hemoglobin and significant decreases in the levels of plasma insulin and hemoglobin. The data obtained confirm the leading role of agrimoniin in the antidiabetic activity of the herb C. palustre and allows us to suggest the use of this plant as a possible dietary adjunct in the treatment of DM and a source of new oral hypoglycaemic agents.<\/jats:p>","DOI":"10.3390\/molecules22010073","type":"journal-article","created":{"date-parts":[[2017,1,2]],"date-time":"2017-01-02T09:54:18Z","timestamp":1483350858000},"page":"73","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Agrimoniin, an Active Ellagitannin from Comarum palustre Herb with Anti-\u03b1-Glucosidase and Antidiabetic Potential in Streptozotocin-Induced Diabetic Rats"],"prefix":"10.3390","volume":"22","author":[{"given":"Nina","family":"Kashchenko","sequence":"first","affiliation":[{"name":"Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, Sakh\u2019yanovoy Street 6, Ulan-Ude 670047, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezhda","family":"Chirikova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, North-Eastern Federal University, 58 Belinsky Street, Yakutsk 677027, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8194-1061","authenticated-orcid":false,"given":"Daniil","family":"Olennikov","sequence":"additional","affiliation":[{"name":"Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, Sakh\u2019yanovoy Street 6, Ulan-Ude 670047, Russia"},{"name":"Department of Biochemistry and Biotechnology, North-Eastern Federal University, 58 Belinsky Street, Yakutsk 677027, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,1,2]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2016). Global Report on Diabetes."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1055\/s-0035-1546131","article-title":"Natural products for the treatment of type 2 diabetes mellitus","volume":"81","author":"Francini","year":"2015","journal-title":"Planta Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"933","DOI":"10.2165\/00003495-200363100-00002","article-title":"Is there a role for \u03b1-glucosidase inhibitors in the prevention of type 2 diabetes mellitus?","volume":"63","author":"Scheen","year":"2003","journal-title":"Drugs"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1007\/BF02936954","article-title":"Safe and effective treatment of diabetes mellitus associated with chronic liver diseases with an alpha-glucosidase inhibitor, acarbose","volume":"32","author":"Kihara","year":"1997","journal-title":"J. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1515\/jpem-2016-0043","article-title":"Idiopathic postprandial hyperinsulinic hypoglycaemia","volume":"29","author":"Melikyan","year":"2016","journal-title":"J. Pediatr. Endocrinol. Metab."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1079\/PNS19970051","article-title":"Nature\u2019s own pharmacy: The diabetes perspective","volume":"56","author":"Gray","year":"1997","journal-title":"Proc. Nutr. Soc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1080\/10408398.2011.561379","article-title":"Advance in dietary polyphenols as \u03b1-glucosidases inhibitors: A review on structure-activity relationship aspect","volume":"53","author":"Xiao","year":"2013","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"8373","DOI":"10.3390\/molecules19068373","article-title":"\u03b1-Glucosidase inhibitory activity of polyphenols from the burs of Castanea mollissima Blume","volume":"19","author":"Zhang","year":"2014","journal-title":"Molecules"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1055\/s-0033-1350925","article-title":"New phenolics from the flowers of Punica granatum and their in vitro \u03b1-glucosidase inhibitory activities","volume":"79","author":"Yuan","year":"2013","journal-title":"Planta Med."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Flores-Bocanegra, L., P\u00e9rez-V\u00e1squez, A., Torres-Piedra, M., Bye, R., Linares, E., and Mata, R. (2015). \u03b1-Glucosidase inhibitors from Vauquelinia corymbosa. Molecules, 20.","DOI":"10.3390\/molecules200815330"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1007\/s12272-009-2207-9","article-title":"Purunusides A-C, \u03b1-glucosidase inhibitory homoisoflavone glucosides from Prunus domestica","volume":"32","author":"Shaheen","year":"2009","journal-title":"Arch. Pharm. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2393","DOI":"10.1002\/jsfa.6564","article-title":"Anthocyanin profiles and biological properties of caneberry (Rubus spp.) press residues","volume":"94","author":"Djilas","year":"2014","journal-title":"J. Sci. Food Agric."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3118","DOI":"10.5897\/AJPP12.408","article-title":"Antioxidant and a-glucosidase inhibitory activity of red raspberry (Harrywaters) fruits in vitro","volume":"6","author":"Yin","year":"2012","journal-title":"Afr. J. Pharm. Pharmacol."},{"key":"ref_14","unstructured":"Rusakova, L.M. (1983). Traditions and Innovations in Life and Culture of the Siberian Peoples, SD RAS."},{"key":"ref_15","unstructured":"Makarov, A.A. (1974). Plant Remedies of the Traditional Yakutian Medicine, Yakutian Scientific Center."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.fitote.2005.03.018","article-title":"Antiinflammatory activity of the pectic polysaccharide from Comarum palustre","volume":"76","author":"Popov","year":"2005","journal-title":"Fitoterapia"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1007\/s10620-005-9034-8","article-title":"Protective effects of comaruman, a pectin of cinquefoil Comarum palustre L. on acetic acid-induced colitis in mice","volume":"51","author":"Popov","year":"2006","journal-title":"Dig. Dis. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1002\/(SICI)1099-1573(199903)13:2<91::AID-PTR350>3.0.CO;2-8","article-title":"Hypoglycaemic activity of four plants used in Chilean popular medicine","volume":"13","author":"Lemus","year":"1999","journal-title":"Phytother. Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Elya, B., Basah, K., Mun\u2019im, A., Yuliastuti, W., Bangun, A., and Septiana, E.K. (2012). Screening of \u03b1-Glucosidase inhibitory activity from some plants of Apocynaceae, Clusiaceae, Euphorbiaceae, and Rubiaceae. J. Biomed. Biotechnol.","DOI":"10.1155\/2012\/281078"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1089\/jmf.2008.0113","article-title":"Evaluation of antihyperglycemia and antihypertension potential of native Peruvian fruits using in vitro models","volume":"12","author":"Pinto","year":"2009","journal-title":"J. Med. Food"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1007\/s10600-016-1754-9","article-title":"Ellagitannins and other phenolic compounds from Comarum palustre","volume":"52","author":"Olennikov","year":"2016","journal-title":"Chem. Nat. Comp."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.jep.2014.05.050","article-title":"HPLC-based activity profiling for antiplasmodial compounds in the traditional Indonesian medicinal plant Carica papaya L","volume":"155","author":"Julianti","year":"2014","journal-title":"J. Ethnopharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s11030-010-9297-7","article-title":"HPLC-based activity profiling for GABAa receptor modulators from the traditional Chinese herbal drug Kushen (Sophora flavescens root)","volume":"15","author":"Yang","year":"2011","journal-title":"Mol. Divers."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2885","DOI":"10.1016\/j.phytochem.2004.07.024","article-title":"HPLC-based bioactivity profiling of plant extracts: A kinetic assay for the identification of monoamine oxidase-A inhibitors using human recombinant monoamine oxidase-A","volume":"65","author":"Dittmann","year":"2004","journal-title":"Phytochemistry"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"899","DOI":"10.2174\/138527206776894401","article-title":"Natural products in drug discovery\u2014Concepts and approaches for tracking bioactivity","volume":"10","author":"Potterat","year":"2006","journal-title":"Curr. Org. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5358","DOI":"10.1021\/ol302548c","article-title":"Antidiabetic ellagitannins from pomegranate flowers: Inhibition of \u03b1-glucosidase and lipogenic gene expression","volume":"20","author":"Yuan","year":"2012","journal-title":"Org. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1002\/(SICI)1097-0010(20000515)80:7<1094::AID-JSFA569>3.0.CO;2-1","article-title":"Proanthocyanidins and tannin-like compounds\u2014Nature, occurrence, dietary intake and effects on nutrition and health","volume":"80","author":"Scalbert","year":"2000","journal-title":"J. Sci. Food Agric."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s11418-010-0402-0","article-title":"Inhibitory effects of Rosa gallica on the digestive enzymes","volume":"64","author":"Ochir","year":"2010","journal-title":"J. Nat. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3109\/09637486.2014.953455","article-title":"Pomegranate ellagitannins inhibit \u03b1-glucosidase activity in vitro and reduce starch digestibility under simulated gastro-intestinal conditions","volume":"66","author":"Bellesia","year":"2015","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.fshw.2014.11.003","article-title":"\u03b1-Glucosidase inhibitors isolated from medicinal plants","volume":"3","author":"Yin","year":"2014","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2760","DOI":"10.1021\/jf0489926","article-title":"Different polyphenolic components of soft fruits inhibit alpha-amylase and alpha-glucosidase","volume":"53","author":"McDougall","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1080\/10408398.2010.548108","article-title":"A review on structure-activity relationship of dietary polyphenols inhibiting \u03b1-amylase","volume":"53","author":"Xiao","year":"2013","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.foodchem.2010.03.088","article-title":"\u03b1-Glucosidase and \u03b1-amylase inhibitory activities of guava leaves","volume":"123","author":"Wang","year":"2010","journal-title":"Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"11463","DOI":"10.1021\/jf903083h","article-title":"Comparative evaluation of quercetin, isoquercetin and rutin as inhibitors of \u03b1-glucosidase","volume":"57","author":"Li","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2458","DOI":"10.1271\/bbb.64.2458","article-title":"Inhibition of alpha-glucosidase and amylase by luteolin, a flavonoid","volume":"64","author":"Kim","year":"2000","journal-title":"Biosci. Biotehnol. Biochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1002\/pca.1360","article-title":"Screening and determination for potential \u03b1-glucosidase inhibitors from leaves of Acanthopanax senticosus Harms by using UF-LC\/MS and ESI-MSn","volume":"24","author":"Zhou","year":"2012","journal-title":"Phytochem. Anal."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1271\/bbb.65.542","article-title":"Inhibitory effects of ellagi- and gallotannins on rat intestinal \u03b1-Glucosidase complexes","volume":"65","author":"Toda","year":"2001","journal-title":"Biosci. Biotech. Biochem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2397","DOI":"10.1016\/0031-9422(88)87004-3","article-title":"Polyphenol complexation\u2014Some thoughts and observatios","volume":"27","author":"Spencer","year":"1988","journal-title":"Phytochemistry"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"11668","DOI":"10.1021\/jf802152z","article-title":"Active compounds from Lagerstroemia speciosa, insulin-like glucose uptake-stimulatory\/inhibitory and adipocyte differentiation-inhibitory activities in 2T3-L1 cells","volume":"56","author":"Bai","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1093\/jn\/135.2.165","article-title":"Tannic acid stimulates glucose transport and inhibits adipocyte differentiation in 3T3-L1 cells","volume":"135","author":"Liu","year":"2005","journal-title":"J. Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1021\/jf9904517","article-title":"Insulin-like biological activity of culinary and medicinal plant aqueous extracts in vitro","volume":"48","author":"Broadhurst","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1515\/pjfns-2016-0001","article-title":"Blood glucose lowering efficacy of strawberry extracts rich in ellagitannins with different degree of polymerization in rats","volume":"66","author":"Jurgonski","year":"2016","journal-title":"Pol. J. Food Nutr. Sci."},{"key":"ref_43","first-page":"243","article-title":"Antihyperglycemic effect of black tea (Camellia sinensis) in rat","volume":"27","author":"Gomes","year":"2001","journal-title":"J. Ethnopharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/biof.5520100211","article-title":"The role of oxidative stress and NF-\u03baB activation in late diabetic complications","volume":"10","author":"Mohamed","year":"1999","journal-title":"BioFactors"},{"key":"ref_45","first-page":"30","article-title":"Islet secretion in a new experiment model for non-insulin dependent diabetes","volume":"5","author":"Weir","year":"1981","journal-title":"Diabetes"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s13105-012-0156-0","article-title":"Effect of rebaudioside A, a diterpenoid on glucose homeostasis in STZ-induced diabetic rats","volume":"68","author":"Saravanan","year":"2012","journal-title":"J. Physiol. Biochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1007\/s00125-007-0886-7","article-title":"The mechanisms of alloxan- and streptozotocin-induced diabetes","volume":"51","author":"Lenzen","year":"2008","journal-title":"Diabetologia"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Pareek, H., Sharma, S., Khajja, B.S., Jain, K., and Jain, G.C. (2009). Evaluation of hypoglycemic and anti-hyperglycemic potential of Tridax procumbens (Linn.). BMC Complement. Altern. Med., 48.","DOI":"10.1186\/1472-6882-9-48"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"5427","DOI":"10.1007\/s13197-014-1676-0","article-title":"2\u03b2-hydroxybetulinic acid 3\u03b2-caprylate: An active principle from Euryale ferox Salisb. seeds with antidiabetic, antioxidant, pancreas & hepatoprotective potential in streptozotocin induced diabetic rats","volume":"52","author":"Ahmed","year":"2015","journal-title":"J. Food Sci. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1017\/S0007114598001834","article-title":"Actions of the traditional anti-diabetic plant, Agrimony eupatoria (agrimony): Effects on hyperglycaemia, cellular glucose metabolism and insulin secretion","volume":"80","author":"Gray","year":"1998","journal-title":"Br. J. Nutr."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.jpba.2013.08.006","article-title":"Development and validation of HPLC-DAD-CAD-MS3 method for qualitative and quantitative standardization of polyphenols in Agrimoniae eupatoriae herba (Ph. Eur)","volume":"86","author":"Granica","year":"2013","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Olennikov, D.N., and Kashchenko, N.I. (2014). Compotential profile and amylase inhibiting activity of phenolic compounds from Calendula officinalis L. leaves. Sci. World J.","DOI":"10.1155\/2014\/654193"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/0304-3959(94)90218-6","article-title":"Study of the sensitivity of the diabetes-induced pain model in rats to a range of analgesics","volume":"57","author":"Courteix","year":"1994","journal-title":"Pain"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1002\/ptr.707","article-title":"Anti-oxidant action of Tinospora cordifolia root extract in alloxan diabetic rats","volume":"15","author":"Stanley","year":"2001","journal-title":"Phytother. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.jep.2003.09.024","article-title":"Protective role of extracts of neem seeds in diabetes caused by streptozotocin in rats","volume":"90","author":"Gupta","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.cbi.2013.11.015","article-title":"Smallanthus macroscyphus: A new source of antidiabetic compounds","volume":"209","author":"Cabrera","year":"2014","journal-title":"Chem. Biol. Interact."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/1\/73\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:25:21Z","timestamp":1760207121000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/1\/73"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,2]]},"references-count":56,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,1]]}},"alternative-id":["molecules22010073"],"URL":"https:\/\/doi.org\/10.3390\/molecules22010073","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,2]]}}}