{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T15:36:34Z","timestamp":1767108994053,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,1,12]],"date-time":"2017-01-12T00:00:00Z","timestamp":1484179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010096","name":"Secretar\u00eda de Educaci\u00f3n P\u00fablica","doi-asserted-by":"publisher","award":["PROMEP\/103.5\/13\/6626"],"award-info":[{"award-number":["PROMEP\/103.5\/13\/6626"]}],"id":[{"id":"10.13039\/100010096","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (CONACyT)","award":["Fellowship No 248596"],"award-info":[{"award-number":["Fellowship No 248596"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The genetically transformed hairy root line LRT 7.31 obtained by infecting leaf explants of Lopezia racemosa Cav with the Agrobacterium rhizogenes strain ATCC15834\/pTDT, was evaluated to identify the anti-inflammatory and cytotoxic compounds reported previously for the wild plant. After several subcultures of the LRT 7.31 line, the bio-guided fractionation of the dichloromethane\u2013methanol (1:1) extract obtained from dry biomass afforded a fraction that showed important in vivo anti-inflammatory, and in vitro cytotoxic activities. Chemical separation of the active fraction allowed us to identify the triterpenes ursolic (1) and oleanolic (2) acids, and (23R)-2\u03b1,3\u03b2,23,28-tetrahydroxy-14,15-dehydrocampesterol (3) as the anti-inflammatory principles of the active fraction. A new molecule 3 was characterized by spectroscopic analysis of its tetraacetate derivative 3a. This compound was not described in previous reports of callus cultures, in vitro germinated seedlings and wild plant extracts of whole L. racemosa plants. The anti-inflammatory and cytotoxic activities displayed by the fraction are associated to the presence of compounds 1\u20133. The present study reports the obtaining of the transformed hairy roots, the bioguided isolation of the new molecule 3, and its structure characterization.<\/jats:p>","DOI":"10.3390\/molecules22010118","type":"journal-article","created":{"date-parts":[[2017,1,12]],"date-time":"2017-01-12T10:02:08Z","timestamp":1484215328000},"page":"118","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A Cytotoxic and Anti-inflammatory Campesterol Derivative from Genetically Transformed Hairy Roots of Lopezia racemosa Cav. (Onagraceae)"],"prefix":"10.3390","volume":"22","author":[{"given":"Norma","family":"Moreno-Anz\u00farez","sequence":"first","affiliation":[{"name":"Centro Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col, Chamilpa C.P. 62209, Cuernavaca, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Marquina","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas-IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col, Chamilpa C.P. 62209, Cuernavaca, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas-IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col, Chamilpa C.P. 62209, Cuernavaca, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2233-5958","authenticated-orcid":false,"given":"Alejandro","family":"Zamilpa","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur (IMSS), Argentina No. 1, Xochitepec Centro C.P. 62790, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Castillo-Espa\u00f1a","sequence":"additional","affiliation":[{"name":"Centro Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col, Chamilpa C.P. 62209, Cuernavaca, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Perea-Arango","sequence":"additional","affiliation":[{"name":"Centro Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col, Chamilpa C.P. 62209, Cuernavaca, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2045-6665","authenticated-orcid":false,"given":"Pilar","family":"Torres","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur (IMSS), Argentina No. 1, Xochitepec Centro C.P. 62790, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maribel","family":"Herrera-Ruiz","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur (IMSS), Argentina No. 1, Xochitepec Centro C.P. 62790, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar","family":"D\u00edaz Garc\u00eda","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur (IMSS), Argentina No. 1, Xochitepec Centro C.P. 62790, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur (IMSS), Argentina No. 1, Xochitepec Centro C.P. 62790, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Arellano-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Centro Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col, Chamilpa C.P. 62209, Cuernavaca, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,1,12]]},"reference":[{"key":"ref_1","first-page":"275","article-title":"Using Hairy Roots for Production of Valuable Plant Secondary Metabolites","volume":"Volume 149","author":"Krull","year":"2000","journal-title":"Filaments in Bioprocesses"},{"key":"ref_2","first-page":"113","article-title":"Silymarin production by hairy root culture of Silybum marianum (L.) Gaertn","volume":"3","author":"Rahnama","year":"2008","journal-title":"Iran. J. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.4236\/ajps.2013.48203","article-title":"Hairy Root Cultures and Plant Regeneration in Solidago nemoralis Transformed with Agrobacterium rhizogenes","volume":"4","author":"Gunjan","year":"2013","journal-title":"Am. J. 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