{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T02:37:16Z","timestamp":1789007836085,"version":"build-2803163510"},"reference-count":43,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T00:00:00Z","timestamp":1484870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Indole-2-carboxylic acid copper complex (ICA-Cu) was successfully prepared and characterized through elemental analysis, IR, UV-Vis, 1H-NMR, TG analysis, and molar conductance, and its molecular formula was [Cu2(C9H6O2N)4(H2O)2]\u00b72H2O. The binding ability of ICA-Cu to calf thymus DNA (CT-DNA) was examined by fluorescence spectrometry and the viscosity method. The results indicated that, upon the addition of increasing amounts of CT-DNA, the excitation and emission intensity of ICA-Cu decreased obviously and the excitation spectra shifted towards a long wavelength. ICA-Cu could displace ethidium bromide (EB) from the EB-DNA system, making the fluorescence intensity of the EB-DNA system decrease sharply; the quenching constant KSV value was 3.99 \u00d7 104 M\u22121. The emission intensity of the ICA-Cu-DNA system was nearly constant, along with the addition of Na+ in a series of concentrations. The fluorescence of the complex could be protected after the complex interacted with DNA. A viscosity measurement further supported the result that the ICA-Cu complex may interact with DNA in an intercalative binding mode. The antioxidant activities of ICA-Cu were evaluated by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, a hydroxyl radical (OH) scavenging assay, and a 2,2\u2032-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. The ICA-Cu exhibited the highest inhibitory effects on the ABTS radical (94% inhibition at 60 \u00b5M), followed by OH and DPPH radicals (the degrees of inhibition being 71% and 56%, respectively). The in vitro cytotoxicity activity of ICA-Cu against two human breast cancer cell lines, MDA-MB-231 and MCF-7, was investigated by 3-[4,5-dimethyltiazol2-yl]-2.5-diphenyl-tetrazolium bromide (MTT) assay and cellular morphological analysis. The results showed that, upon increasing the concentration of ICA-Cu, an increase was observed in growth-inhibitory activity and the inhibition percentage were greater than 90% at 20 \u00b5M in both cell lines. Also, cellular morphological changes in the two cell lines agreed with the cytotoxicity results.<\/jats:p>","DOI":"10.3390\/molecules22010171","type":"journal-article","created":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T10:10:12Z","timestamp":1484907012000},"page":"171","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["In Vitro DNA-Binding, Anti-Oxidant and Anticancer Activity of Indole-2-Carboxylic Acid Dinuclear Copper(II) Complexes"],"prefix":"10.3390","volume":"22","author":[{"given":"Xiangcong","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy, Jining Medical University, 669 Xueyuan Road, Rizhao 276800, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maocai","family":"Yan","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jining Medical University, 669 Xueyuan Road, Rizhao 276800, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qibao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jining Medical University, 669 Xueyuan Road, Rizhao 276800, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huannan","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jining Medical University, 669 Xueyuan Road, Rizhao 276800, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyang","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jining Medical University, 669 Xueyuan Road, Rizhao 276800, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jining Medical University, 669 Xueyuan Road, Rizhao 276800, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jining Medical University, 669 Xueyuan Road, Rizhao 276800, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jining Medical University, 669 Xueyuan Road, Rizhao 276800, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3322\/caac.21338","article-title":"Cancer Statistics in China, 2015","volume":"66","author":"Chen","year":"2016","journal-title":"CA Cancer J. 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