{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:46:59Z","timestamp":1785332819016,"version":"3.55.0"},"reference-count":56,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,1,23]],"date-time":"2017-01-23T00:00:00Z","timestamp":1485129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["2162030"],"award-info":[{"award-number":["2162030"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Academy of Science and Technology","award":["2014"],"award-info":[{"award-number":["2014"]}]},{"name":"National High Technology Research and Development Program of China","award":["2014AA021705"],"award-info":[{"award-number":["2014AA021705"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In this paper we report a facile method for preparing co-immobilized enzyme and magnetic nanoparticles (MNPs) using metal coordinated hydrogel nanofibers. Candida rugosa lipase (CRL) was selected as guest protein. For good aqueous dispersity, low price and other unique properties, citric acid-modified magnetic iron oxide nanoparticles (CA-Fe3O4 NPs) have been widely used for immobilizing enzymes. As a result, the relative activity of CA-Fe3O4@Zn\/AMP nanofiber-immobilized CRL increased by 8-fold at pH 10.0 and nearly 1-fold in a 50 \u00b0C water bath after 30 min, compared to free CRL. Moreover, the immobilized CRL had excellent long-term storage stability (nearly 80% releative activity after storage for 13 days). This work indicated that metal-nucleotide nanofibers could efficiently co-immobilize enzymes and MNPs simultaneously, and improve the stability of biocatalysts.<\/jats:p>","DOI":"10.3390\/molecules22010179","type":"journal-article","created":{"date-parts":[[2017,1,23]],"date-time":"2017-01-23T10:40:33Z","timestamp":1485168033000},"page":"179","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Co-Immobilization of Enzymes and Magnetic Nanoparticles by Metal-Nucleotide Hydrogelnanofibers for Improving Stability and Recycling"],"prefix":"10.3390","volume":"22","author":[{"given":"Chunfang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Environment Protection and Detection, Beijing Industrial Technician College, Beijing 100023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhui","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinying","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Liang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,1,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.colsurfb.2011.08.031","article-title":"Improving immobilization of lipase onto magnetic microspheres with moderate hydrophobicity\/hydrophilicity","volume":"89","author":"Zhang","year":"2012","journal-title":"Colloids Surf. 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