{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:52:25Z","timestamp":1790009545145,"version":"4.0.1"},"reference-count":54,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T00:00:00Z","timestamp":1486512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Many Helianthemum species (Cistaceae) are recognized for their various medicinal virtues. Helianthemum ruficomum is an endemic species to the septentrional Sahara on which no report is available so far. The purpose of this work was to investigate the chemical composition and the radical scavenging capacity of this species and its isolated components. Collected from Mougheul (south-west of Algeria), the aerial parts were macerated with 80% EtOH\/H2O, after evaporation, the remaining extract was diluted with H2O and extracted with petroleum ether, chloroform, ethyl acetate and n-butanol. EtOAc and n-BuOH extracts were evaluated for their free radical scavenging capacity by on-line HPLC-ABTS\u2022+ assay. The obtained data which were confirmed by TEAC and ORAC assays, allowed guiding the fractionation of these extracts by CC, TLC and reverse phase HPLC. Among the components, 14 were isolated and identified by spectroscopic analyses: protocatechuic acid (1), trans-tiliroside (2), cis-tiliroside (3), astragalin (4), picein (7), vanillic acid 4-O-\u03b2-d-glucopyranoside (8), lavandoside (9), 4-hydroxybenzoic acid 4-O-\u03b2-d-glucopyranoside (10), nicotiflorin (11), rutin (12), vicenin-2 (13), narcissin (14) and stigmasterol (5) and \u03b2-sitosterol (6) as a mixture (71% and 29%, respectively). Compounds 5, 7, 8, 9, 10 and 14 were new for the genus Helianthemum. The antioxidant power of all the isolated compounds was also evaluated by HPLC-ABTS\u2022+, TEAC and ORAC assays. The results clearly indicated high antioxidant potential of the extracts and tested compounds of this species especially, compounds 1, 4, 8, 9, 10 and 12.<\/jats:p>","DOI":"10.3390\/molecules22020239","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T05:27:09Z","timestamp":1486704429000},"page":"239","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["On-Line Screening, Isolation and Identification of Antioxidant Compounds of Helianthemum ruficomum"],"prefix":"10.3390","volume":"22","author":[{"given":"Yasmine","family":"Chemam","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche Valorisation des Ressources Naturelles, Mol\u00e9cules Bioactives et Analyses Physicochimiques et Biologiques, Universit\u00e9 des Fr\u00e8res Mentouri, Constantine 1, Route d\u2019A\u00efn El Bey, 25000 Constantine, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2298-2466","authenticated-orcid":false,"given":"Samir","family":"Benayache","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche Valorisation des Ressources Naturelles, Mol\u00e9cules Bioactives et Analyses Physicochimiques et Biologiques, Universit\u00e9 des Fr\u00e8res Mentouri, Constantine 1, Route d\u2019A\u00efn El Bey, 25000 Constantine, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Marchioni","sequence":"additional","affiliation":[{"name":"Chimie Analytique des Mol\u00e9cules Bioactives, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS\/UDS), 74 route du Rhin, 67400 Illkirch, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjie","family":"Zhao","sequence":"additional","affiliation":[{"name":"Chimie Analytique des Mol\u00e9cules Bioactives, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS\/UDS), 74 route du Rhin, 67400 Illkirch, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Mosset","sequence":"additional","affiliation":[{"name":"Institut des Sciences Chimiques de Rennes, CNRS UMR 6226, Universit\u00e9 de Rennes 1, 263 Avenue du G\u00e9n\u00e9ral Leclerc, CS 74205, 35042 Rennes CEDEX, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadila","family":"Benayache","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche Valorisation des Ressources Naturelles, Mol\u00e9cules Bioactives et Analyses Physicochimiques et Biologiques, Universit\u00e9 des Fr\u00e8res Mentouri, Constantine 1, Route d\u2019A\u00efn El Bey, 25000 Constantine, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1016\/j.foodchem.2009.11.022","article-title":"Umbelliferone\u2014An antioxidant isolated from Acacia nilotica (L.) 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