{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:44:01Z","timestamp":1788925441846,"version":"build-2803163510"},"reference-count":41,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2017,5,6]],"date-time":"2017-05-06T00:00:00Z","timestamp":1494028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Helicobacter pylori-associated gastritis is a major threat to public health and Polygonum capitatum (PC) may have beneficial effects on the disease. However, the molecular mechanism remains unknown. Quercetin was isolated from PC and found to be a main bioactive compound. The effects of quercetin on human gastric cancer cells GES-1 were determined by xCELLigence. H. pylori-infected mouse models were established. All mice were divided into three groups: control (CG, healthy mice), model (MG, H. pylori infection) and quercetin (QG, mouse model treated by quercetin) groups. IL-8 (interleukin-8) levels were detected via enzyme-linked immunosorbent assay (ELISA). Cell cycle and apoptosis were measured by flow cytometry (FCM). Quantitative reverse transcription PCR (qRT-PCR) and Western Blot were used to detect the levels of p38MAPK (38-kD tyrosine phosphorylated protein kinase), apoptosis regulator BCL-2-associated protein X (BAX) and B cell lymphoma gene 2 (BCL-2). The levels of IL-8 were increased by 8.1-fold in a MG group and 4.3-fold in a QG group when compared with a CG group. In a MG group, G0\u2013G1(phases of the cell cycle)% ratio was higher than a CG group while S phase fraction was lower in a model group than in a control group (p &lt; 0.01). After quercetin treatment, G0\u2013G1% ratio was lower in a QG group than a MG group while S phase fraction was higher than a MG group (p &lt; 0.01). Quercetin treatment reduced the levels of p38MAPK and BAX, and increased the levels of BCL-2 when compared with a MG group (p &lt; 0.05). Quercetin regulates the balance of gastric cell proliferation and apoptosis to protect against gastritis. Quercetin protects against gastric inflammation and apoptosis associated with H. pylori infection by affecting the levels of p38MAPK, BCL-2 and BAX.<\/jats:p>","DOI":"10.3390\/molecules22050744","type":"journal-article","created":{"date-parts":[[2017,5,8]],"date-time":"2017-05-08T11:45:16Z","timestamp":1494243916000},"page":"744","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Quercetin from Polygonum capitatum Protects against Gastric Inflammation and Apoptosis Associated with Helicobacter pylori Infection by Affecting the Levels of p38MAPK, BCL-2 and BAX"],"prefix":"10.3390","volume":"22","author":[{"given":"Shu","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, School of Medical Laboratory Sciences, Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqin","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, School of Medical Laboratory Sciences, Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"He","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, School of Medical Laboratory Sciences, Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, School of Medical Laboratory Sciences, Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoxun","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, School of Medical Laboratory Sciences, Guizhou Medical University, Guiyang 550004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,5,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.3164\/jcbn.15-38","article-title":"Repressed TGF-beta signaling through CagA-Smad3 interaction as pathogenic mechanisms of Helicobacter pylori-associated gastritis","volume":"57","author":"Nguyen","year":"2015","journal-title":"J. Clin. Biochem. Nutr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1097\/MIB.0000000000000595","article-title":"Inverse Association between Helicobacter pylori Gastritis and Microscopic Colitis","volume":"22","author":"Sonnenberg","year":"2016","journal-title":"Inflamm. Bowel. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1097\/MCG.0b013e318266f6cf","article-title":"Meta-analysis of the efficacy and safety of Lactobacillus-containing and Bifidobacterium-containing probiotic compound preparation in Helicobacter pylori eradication therapy","volume":"47","author":"Wang","year":"2013","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2453254","DOI":"10.1155\/2017\/2453254","article-title":"Cost-Effectiveness Analysis of Helicobacter pylori Diagnostic Methods in Patients with Atrophic Gastritis","volume":"2017","author":"Omata","year":"2017","journal-title":"Gastroenterol. Res. Pract."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Cheng, H.C., Tsai, Y.C., Yang, H.B., Yeh, Y.C., Chang, W.L., Kuo, H.Y., Lu, C.C., and Sheu, B.S. (2017). The corpus-predominant gastritis index can be an early and reversible marker to identify the gastric cancer risk of Helicobacter pylori-infected nonulcer dyspepsia. Helicobacter.","DOI":"10.1111\/hel.12385"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.3748\/wjg.v23.i9.1521","article-title":"Host pathogen interactions in Helicobacter pylori related gastric cancer","volume":"23","author":"Chmiela","year":"2017","journal-title":"World J. Gastroenterol."},{"key":"ref_7","first-page":"S6","article-title":"Helicobacter pylori and gastric cancer: A state of the art review","volume":"8","author":"Ishaq","year":"2015","journal-title":"Gastroenterol. Hepatol. Bed Bench"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1242\/dmm.027649","article-title":"Helicobacter pylori-induced gastric pathology: Insights from in vivo and ex vivo models","volume":"10","author":"Burkitt","year":"2017","journal-title":"Dis. Model. Mech."},{"key":"ref_9","first-page":"2","article-title":"A primary esophageal MALT lymphoma patient with Helicobacter pylori infection achieved complete remission after H. pylori eradication without anti-lymphoma treatment","volume":"7","author":"Moriya","year":"2017","journal-title":"Leuk. Res. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3","DOI":"10.5152\/tjg.2016.0278","article-title":"Use of probiotics as an adjuvant to sequential H. pylori eradication therapy: Impact on eradication rates, treatment resistance, treatment-related side effects, and patient compliance","volume":"28","author":"Cekin","year":"2017","journal-title":"Turk. J. Gastroenterol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"229","DOI":"10.4166\/kjg.2010.56.4.229","article-title":"Antibiotics-associated diarrhea and other gastrointestinal abnormal responses regarding Helicobacter pylori eradication","volume":"56","author":"Kwon","year":"2010","journal-title":"Korean J. Gastroenterol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.jgar.2016.12.019","article-title":"Clarithromycin resistance in Helicobacter pylori and its molecular determinants in northern Spain, 2013\u20132015","volume":"9","author":"Tamayo","year":"2017","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"85","DOI":"10.2147\/IDR.S123798","article-title":"Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru","volume":"10","author":"Boehnke","year":"2017","journal-title":"Infect. Drug Resist."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Meng, F., Yang, S., Wang, X., Chen, T., Wang, X., Tang, X., Zhang, R., and Shen, L. (2017). Reclamation of Chinese herb residues using probiotics and evaluation of their beneficial effect on pathogen infection. J. Infect. Public Health, in press.","DOI":"10.1016\/j.jiph.2016.11.013"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1080\/14756366.2016.1178639","article-title":"Chinese herb medicine against Sortase A catalyzed transformations, a key role in gram-positive bacterial infection progress","volume":"31","author":"Si","year":"2016","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"S155","DOI":"10.1111\/j.1440-1746.2010.06232.x","article-title":"Sofalcone, a gastroprotective drug, promotes gastric ulcer healing following eradication therapy for Helicobacter pylori: A randomized controlled comparative trial with cimetidine, an H2-receptor antagonist","volume":"25","author":"Higuchi","year":"2010","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-2036.18.s1.18.x","article-title":"Comparison of the effects of rebamipide with those of cimetidine on chronic gastritis associated with Helicobacter pylori in Mongolian gerbils","volume":"18","author":"Higuchi","year":"2003","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_18","first-page":"827","article-title":"Failure of cimetidine prophylaxis in the critically ill patient: Two fatal cases of duodenal perforation","volume":"77","author":"Previti","year":"1980","journal-title":"J. Med. Soc. N. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.1002\/jssc.201300291","article-title":"Development and validation of HPLC coupled with triple quadrupole MS for the simultaneous determination of six phenolic acids, six flavonoids, and a lignan in Polygonum capitatum","volume":"36","author":"Zhang","year":"2013","journal-title":"J. Sep. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/j.fitote.2007.05.003","article-title":"A new chromone glycoside from Polygonum capitatum","volume":"78","author":"Li","year":"2007","journal-title":"Fitoterapia"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1016\/j.jep.2011.01.050","article-title":"Antibacterial and anti-inflammatory effects of extracts and fractions from Polygonum capitatum","volume":"134","author":"Liao","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"ref_22","first-page":"1749","article-title":"Pharmacological and other Bioactivities of the Genus Polygonum\u2014A Review","volume":"13","author":"Dong","year":"2014","journal-title":"Trop. J. Pharm. Res."},{"key":"ref_23","first-page":"995","article-title":"Chemical constituents from Polygonum capitatum and their antioxidation activities in vitro","volume":"31","author":"Liu","year":"2008","journal-title":"Zhong Yao Cai"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/j.1574-695X.2011.00833.x","article-title":"Upregulation of progranulin by Helicobacter pylori in human gastric epithelial cells via p38MAPK and MEK1\/2 signaling pathway: Role in epithelial cell proliferation and migration","volume":"63","author":"Wang","year":"2011","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"258","DOI":"10.5604\/17322693.1198272","article-title":"EGFR and Bcl-2 in gastric mucosa of children infected with Helicobacter pylori","volume":"70","author":"Ryszczuk","year":"2016","journal-title":"Postepy Hig. Med. Dosw."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s10620-008-0699-7","article-title":"Effects of Helicobacter pylori infection on the expressions of Bax and Bcl-2 in patients with chronic gastritis and gastric cancer","volume":"55","author":"Bartchewsky","year":"2010","journal-title":"Dig. Dis. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Huang, Y., Sun, H.-Y., Qin, X.-L., Li, Y.-J., Liao, S.-G., Gong, Z.-P., Lu, Y., Wang, Y.-L., Wang, A.-M., and Lan, Y.-Y. (2017). A UPLC-MS\/MS Method for Simultaneous Determination of Free and Total Forms of a Phenolic Acid and Two Flavonoids in Rat Plasma and Its Application to Comparative Pharmacokinetic Studies of Polygonum capitatum Extract in Rats. Molecules, 22.","DOI":"10.3390\/molecules22030353"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0022-1759(94)90396-4","article-title":"A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: An alternative to [3H] thymidine incorporation assay","volume":"170","author":"Ahmed","year":"1994","journal-title":"J. Immunol. Methods"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.tim.2003.11.005","article-title":"Helicobacter pylori-induced epithelial cell signalling in gastric carcinogenesis","volume":"12","author":"Naumann","year":"2004","journal-title":"Trends. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.ijantimicag.2015.08.010","article-title":"Linezolid susceptibility in Helicobacter pylori, including strains with multidrug resistance","volume":"46","author":"Boyanova","year":"2015","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"164","DOI":"10.5662\/wjm.v5.i3.164","article-title":"Prevalence of antibiotic resistance in Helicobacter pylori: A recent literature review","volume":"5","author":"Ghotaslou","year":"2015","journal-title":"World J. Methodol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"568106","DOI":"10.1155\/2012\/568106","article-title":"Efficacy and side effects of chinese herbal medicine for menopausal symptoms: A critical review","volume":"2012","author":"Xu","year":"2012","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1111\/j.1443-9573.2004.00163.x","article-title":"Investigation of the sensitivities of distinct gastric cancer cells to parvovirus H-1 induced cytotoxicity","volume":"5","author":"Ran","year":"2004","journal-title":"Chin. J. Dig. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"12832","DOI":"10.1128\/JVI.01216-10","article-title":"Influenza A virus replication induces cell cycle arrest in G0\/G1 phase","volume":"84","author":"He","year":"2010","journal-title":"J. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1758-907X-2-7","article-title":"Helicobacter pylori interferes with an embryonic stem cell micro RNA cluster to block cell cycle progression","volume":"2","author":"Belair","year":"2011","journal-title":"Silence"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"10920","DOI":"10.1016\/S0021-9258(18)82073-7","article-title":"Structure-function analysis of the Bcl-2 oncoprotein. Addition of a heterologous transmembrane domain to portions of the Bcl-2 beta protein restores function as a regulator of cell survival","volume":"268","author":"Tanaka","year":"1993","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1007\/s10620-015-3836-0","article-title":"Cortactin Mediates Apoptosis of Gastric Epithelial Cells Induced by VacA Protein of Helicobacter pylori","volume":"61","author":"Chang","year":"2016","journal-title":"Dig. Dis. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/j.bbrc.2015.05.059","article-title":"Compound 13, an alpha1-selective small molecule activator of AMPK, inhibits Helicobacter pylori-induced oxidative stresses and gastric epithelial cell apoptosis","volume":"463","author":"Zhao","year":"2015","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1172\/JCI316","article-title":"Stretch-mediated release of angiotensin II induces myocyte apoptosis by activating p53 that enhances the local renin-angiotensin system and decreases the Bcl-2-to-Bax protein ratio in the cell","volume":"101","author":"Leri","year":"1998","journal-title":"J. Clin. Investig."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"281","DOI":"10.4067\/S0716-97602006000200010","article-title":"Antioxidant activity of extract from Polygonum aviculare L.","volume":"39","author":"Hsu","year":"2006","journal-title":"Biol. Res."},{"key":"ref_41","first-page":"253","article-title":"Preparation of suspension of gastric mucous membrane single cell and expression of cyclins in cells","volume":"11","author":"Cao","year":"2008","journal-title":"Zhonghua Wei Chang Wai Ke Za Zhi"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/5\/744\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:34:49Z","timestamp":1760207689000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/5\/744"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,6]]},"references-count":41,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2017,5]]}},"alternative-id":["molecules22050744"],"URL":"https:\/\/doi.org\/10.3390\/molecules22050744","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,6]]}}}