{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:07:13Z","timestamp":1790971633079,"version":"4.1.0"},"reference-count":32,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T00:00:00Z","timestamp":1494288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>In this work, flavonoid fraction from the leaves of Crataegus pinnatifida was separated into its seven main constituents using a combination of HSCCC coupled with pre-HPLC. In the first step, the total flavonoid extract was subjected to HSCCC with a two-solvent system of chloroform\/methanol\/water\/n-butanol (4:3:2:1.5, v\/v), yielding four pure compounds, namely (\u2013)-epicatechin (1), quercetin-3-O-(2,6-di-\u03b1-l-rhamnopyranosyl)-\u03b2-d-galactopyranoside (2), 4\u2032\u2032-O-glucosylvitexin (3) and 2\u2032\u2032-O-rhamnosylvitexin (4) as well as a mixture of three further flavonoids. An extrusion mode was used to rapidly separate quercetin-3-O-(2,6-di-\u03b1-l-rhamnopyranosyl)-\u03b2-d-galactopyranoside with a big KD-value. In the second step, the mixture that resulted from HSCCC was separated by pre-HPLC, resulting in three pure compounds including: vitexin (5), hyperoside (6) and isoquercitrin (7). The purities of the isolated compounds were established to be over 98%, as determined by HPLC. The structures of these seven flavonoids were elucidated by ESI-MS and NMR spectroscopic analyses.<\/jats:p>","DOI":"10.3390\/molecules22050767","type":"journal-article","created":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T11:50:48Z","timestamp":1494330648000},"page":"767","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["An Efficient Method for the Preparative Isolation and Purification of Flavonoids from Leaves of Crataegus pinnatifida by HSCCC and Pre-HPLC"],"prefix":"10.3390","volume":"22","author":[{"given":"Lei","family":"Wen","sequence":"first","affiliation":[{"name":"College of Pharmacy, Shandong University of Traditional Chinese Medicine, 4655 Daxue Road, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunliang","family":"Lin","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruimin","family":"Lv","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijiao","family":"Yan","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinqian","family":"Yu","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengqiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1163-3597","authenticated-orcid":false,"given":"Xiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daijie","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,5,9]]},"reference":[{"key":"ref_1","unstructured":"Committee of National Pharmacopoeia (2015). Pharmacopoeia of P.R. China, Chemical Industry Press."},{"key":"ref_2","first-page":"2955","article-title":"Study on effects of hawthorn leaves extract on fatty liver in rats","volume":"26","author":"Wang","year":"2011","journal-title":"Chin. J. Tradit. Chin. Med. Pharm."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1111\/jphp.12225","article-title":"Effects of vitexin-2\u2032\u2032-O-rhamnoside and vitexin-4\u2032\u2032-O-glucoside on growth and oxidative stress-induced cell apoptosis of human adipose-derived stem cells","volume":"66","author":"Wei","year":"2014","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2948","DOI":"10.1271\/bbb.60361","article-title":"Polyphenolic content and physiological activities of Chinese hawthorn extracts","volume":"70","author":"Cui","year":"2006","journal-title":"Biosci Biotechnol Biochem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.3390\/molecules19021685","article-title":"Crataegus pinnatifida: Chemical constituents, pharmacology, and potential applications","volume":"19","author":"Wu","year":"2014","journal-title":"Molecules"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1002\/jsfa.5671","article-title":"Composition and health effects of phenolic compounds in hawthorn (Crataegus spp.) of different origins","volume":"92","author":"Yang","year":"2012","journal-title":"J. Sci. Food Agric."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/S0031-9422(01)00170-4","article-title":"Flavonoid ketohexosefuranosides from the leaves of Crataegus pinnatifida Bge. var. major N.E.Br.","volume":"57","author":"Zhang","year":"2001","journal-title":"Phytochemistry"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1080\/1028602021000054982","article-title":"C-Glucoside flavonoids from the leaves of Crataegus pinnatifida Bge. var. major N.E.Br.","volume":"5","author":"Zhang","year":"2003","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_9","first-page":"337","article-title":"Two New C-glucoside flavonoids from leaves of Crataegus pinnatifida Bge. var. major N.E.Br.","volume":"13","author":"Zhang","year":"2002","journal-title":"Chin. Chem. Lett."},{"key":"ref_10","first-page":"754","article-title":"Chemical constituents from the leaves of Crataegus pinnatifida Bge. Var. major N.E.Br.","volume":"36","author":"Zhang","year":"2001","journal-title":"Acta Pharm. Sin."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1016\/S0031-9422(02)00172-3","article-title":"Isolation and identification of oligomeric procyanidins from Crataegus leaves and flowers","volume":"60","author":"Vuorela","year":"2002","journal-title":"Phytochemistry"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1007\/BF00564585","article-title":"Pinnatifidin-A new flavonol glycoside from Crataegus pinnatifida","volume":"8","author":"Bykov","year":"1972","journal-title":"Chem. Nat. Compd."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1016\/j.bse.2010.09.010","article-title":"Monoterpene and lignan glycosides in the leaves of Crataegus pinnatifida","volume":"38","author":"Gao","year":"2010","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bse.2012.12.001","article-title":"Monoterpene and sesquilignan compounds from the leaves of Crataegus pinnatifida","volume":"48","author":"Huang","year":"2013","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.chroma.2014.07.057","article-title":"Chiral ligand exchange high-speed countercurrent chromatography: Mechanism and application in enantioseparation of aromatic \u03b1-hydroxyl acids","volume":"1360","author":"Tong","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.seppur.2016.02.026","article-title":"An approach based upon the consecutive separation and the economical two-phase solvent system preparation using UNIFAC mathematical model for increasing the yield of high-speed counter-current chromatography","volume":"162","author":"Kang","year":"2016","journal-title":"Sep. Purif. Technol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1672","DOI":"10.3390\/molecules18021672","article-title":"The Passiflora tripartita (Banana Passion) Fruit: A Source of bioactive flavonoid C-glycosides isolated by HSCCC and characterized by HPLC-DAD-ESI\/MS\/MS","volume":"18","author":"Simirgiotis","year":"2013","journal-title":"Molecules"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"15648","DOI":"10.3390\/molecules181215648","article-title":"Preparative isolation and purification of five flavonoid glycosides and one benzophenone galloyl glycoside from Psidium guajava by high-speed counter-current chromatography (HSCCC)","volume":"18","author":"Zhu","year":"2013","journal-title":"Molecules"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.seppur.2016.04.005","article-title":"Computation-aided separation of seven components from Spirodela polyrrhiza (L.) via counter-current chromatography","volume":"165","author":"Ren","year":"2016","journal-title":"Sep. Purif. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.jchromb.2015.12.051","article-title":"Separation and preparation of 6-gingerol from molecular distillation residue of Yunnan ginger rhizomes by high-speed counter-current chromatography and the antioxidant activity of ginger oils in vitro","volume":"1011","author":"Gan","year":"2016","journal-title":"J. Chromatogr. B"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1080\/01496395.2015.1117101","article-title":"Separation and purification of six biosurfactant rhamnolipids by high-speed countercurrent chromatography utilizing novel solvent selection method","volume":"51","author":"Zhang","year":"2016","journal-title":"Sep. Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3339","DOI":"10.1002\/jssc.201100573","article-title":"An efficient combination of supercritical fluid extraction and high-speed counter-current chromatography to extract and purify (E)- and (Z)-diastereomers of \u03b1-asarone and \u03b2-asarone from Acorus tatarinowii Schott","volume":"34","author":"Wang","year":"2011","journal-title":"J. Sep. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1080\/10826070903163008","article-title":"Isolation and purification of three flavonoids from the hawthorn leaves by high speed countercurrent chromatography, combined with isocratic preparative reversed-phase high performance liquid chromatography","volume":"32","author":"Xu","year":"2009","journal-title":"J. Liq. Chromatogr. Relat. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.chroma.2004.12.044","article-title":"Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography","volume":"1065","author":"Ito","year":"2005","journal-title":"J. Chromatogr. A"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.chroma.2004.12.026","article-title":"Preparative isolation and purification of three flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai by high-speed counter-current chromatography","volume":"1064","author":"Liu","year":"2005","journal-title":"J. Chromatogr. A"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/0031-9422(93)85370-7","article-title":"Nuclear magnetic resonance studies of 5,7-dihydroxyflavonoids","volume":"34","author":"Shen","year":"1993","journal-title":"Phytochemistry"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1080\/10286020290019659","article-title":"Flavonol glycosides from Lysimachia capillipes","volume":"4","author":"Xie","year":"2002","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1016\/S0031-9422(00)97951-2","article-title":"Two flavonol glycosides from Lysimachia fortune","volume":"28","author":"Yasukawa","year":"1989","journal-title":"Phytochemisty"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1002\/pca.853","article-title":"Isolation and identification of flavonoids, including flavone rotamers, from the herbal drug \u2018Crataegi folium cum flore\u2019 (hawthorn)","volume":"16","author":"Rayyan","year":"2005","journal-title":"Phytochem. Anal."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1016\/j.jep.2012.05.062","article-title":"Vitexin and isovitexin from the leaves of Ficus deltoidea with in-vivo \u03b1-glucosidase inhibition","volume":"142","author":"Choo","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2487","DOI":"10.1016\/j.jchromb.2009.06.026","article-title":"Isolation and purification of three flavonoid glycosides from the leaves of Nelumbo nucifera (Lotus) by high-speed counter-current chromatography","volume":"877","author":"Deng","year":"2009","journal-title":"J. Chromatogr. B"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jchromb.2017.01.041","article-title":"Purification of quercetin-3-O-sophoroside and isoquercitrin from Poacynum hendersonii leaves using macroporous resins followed by Sephadex LH-20 column chromatography","volume":"1048","author":"Li","year":"2017","journal-title":"J. Chromatogr. B"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/5\/767\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:35:14Z","timestamp":1760207714000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/5\/767"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,9]]},"references-count":32,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2017,5]]}},"alternative-id":["molecules22050767"],"URL":"https:\/\/doi.org\/10.3390\/molecules22050767","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,9]]}}}