{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T15:04:58Z","timestamp":1774969498152,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2017,6,2]],"date-time":"2017-06-02T00:00:00Z","timestamp":1496361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81220108028"],"award-info":[{"award-number":["81220108028"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81073003"],"award-info":[{"award-number":["81073003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["S2013010012373"],"award-info":[{"award-number":["S2013010012373"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2014A030306043"],"award-info":[{"award-number":["2014A030306043"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Major Scientific and Program of Introducing Talents of Discipline to Universities","award":["B13038"],"award-info":[{"award-number":["B13038"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Xian-Ling-Gu-Bao capsule (XLGB), a famous traditional Chinese medicine prescription, is extensively used for the treatment of osteoporosis in China. However, few studies on the holistic quality control of XLGB have been reported. In this study, a reliable method using 18 representative components in XLGB was successfully established and applied to evaluate 34 batches of XLGB samples by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC\/Q-TOF-MS). The choice of quantitative markers mostly followed four principles, i.e., absorbed components in plasma, bioactive compounds with in vitro anti-osteoporosis activity, those derived from multiple individual medicinal herbs in XLGB with multiple representative structure types, and quantitative chemical markers in the Chinese Pharmacopoeia. The results showed chemical consistency was good except for individual batches. Multivariate statistical analysis indicated that asperosaponin VI from Radix Dipsaci, epimedin C, magnoflorine, and icariin from Herba Epimedii as well as timosaponin BII from Rhizoma Anemarrhenae varied significantly in multiple samples, which hinted an assay for these four components should be completed during all of the manufacturing processes. Taken together, this study provided a feasible method for holistic quality control of XLGB by multiple chemical markers, which could play a vital role in guaranteeing the safety, effectiveness, and controllability of administering the capsules as a medication in clinics.<\/jats:p>","DOI":"10.3390\/molecules22060927","type":"journal-article","created":{"date-parts":[[2017,6,2]],"date-time":"2017-06-02T10:20:44Z","timestamp":1496398844000},"page":"927","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Simultaneous Quantification of Multiple Representative Components in the Xian-Ling-Gu-Bao Capsule by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5999-4466","authenticated-orcid":false,"given":"Zhi-Hong","family":"Yao","sequence":"first","affiliation":[{"name":"College of Pharmacy, Jinan University, Guangzhou 510632, China"},{"name":"Guangdong Provincial Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8989-1986","authenticated-orcid":false,"given":"Zi-Fei","family":"Qin","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Jinan University, Guangzhou 510632, China"},{"name":"Guangdong Provincial Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China"},{"name":"G Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Jinan University, Guangzhou 510632, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Meng","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Jinan University, Guangzhou 510632, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Dai","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Jinan University, Guangzhou 510632, China"},{"name":"Guangdong Provincial Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Luan","family":"Wang","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopedics and Traumatology, the Chinese University of Hong Kong, Satin, N.T. Hong Kong SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Qin","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research Laboratory, Department of Orthopedics and Traumatology, the Chinese University of Hong Kong, Satin, N.T. Hong Kong SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Cai","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Jinan University, Guangzhou 510632, China"},{"name":"Guangdong Provincial Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China"},{"name":"G Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Sheng","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Jinan University, Guangzhou 510632, China"},{"name":"Guangdong Provincial Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China"},{"name":"G Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1126\/science.299.5604.188","article-title":"The new face of traditional Chinese medicine","volume":"299","author":"Normile","year":"2003","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/480S82a","article-title":"TCM: Made in China","volume":"480","author":"Cheung","year":"2011","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.aca.2009.10.024","article-title":"Recent analytical approaches in quality control of traditional Chinese medicines\u2014A review","volume":"657","author":"Jiang","year":"2010","journal-title":"Anal. 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