{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:57:09Z","timestamp":1787029029930,"version":"build-2736575974"},"reference-count":27,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2017,6,16]],"date-time":"2017-06-16T00:00:00Z","timestamp":1497571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011264","name":"FP7 People: Marie-Curie Actions","doi-asserted-by":"publisher","award":["Grant agreement no. 286\u200a287 NATPROTEC"],"award-info":[{"award-number":["Grant agreement no. 286\u200a287 NATPROTEC"]}],"id":[{"id":"10.13039\/100011264","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Progetto Campania Research in Experimental Medicine","award":["POR Campania FSE 2007-2013"],"award-info":[{"award-number":["POR Campania FSE 2007-2013"]}]},{"name":"FARB 2014","award":["ORSA147579"],"award-info":[{"award-number":["ORSA147579"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Background: Doronicum austriacum Jacq., Asteraceae, is a plant which is used in traditional alpine medicine. Historical sources describe the medical use of the root, but up until now only a few studies evaluated its pharmacological properties. The evaluation of the dichloromethane extract, and its major compounds for their anti-inflammatory and anti-oxidant potential was performed in macrophages J774A.1 and C6 astrocytes. Nitric oxide (NO) and reactive oxygen species (ROS) release, as well as nitrotyrosine formation, were evaluated. Moreover, in order to evaluate the potential anti-proliferative activity, under the same experimental conditions, 3-(4,5-dimethyltiazol-2yl)-2,5-phenyl-2H-tetrazolium bromide (MTT) assay was also performed. Our results indicate that Doronicum austriacum has a significant effect in inhibiting both pro-inflammatory and pro-oxidative mediators. All isolated compounds were able to significantly inhibit NO and ROS release both in macrophage and in astrocytes cells, even if the effect was more pronounced in macrophage. In particular, among the tested compounds, 6,12-dihydroxy-(\u2212)-2S-tremetone exerted stronger activity. Both extract and single compounds did not affect cellular viability. This study provides evidence for the pharmacological anti-inflammatory and anti-oxidant potential of Doronicum austriacum extract. These effects could be due to the activity of its major constituents and subsequent identification of benzofurans as a promising compound class to combat inflammation and related diseases.<\/jats:p>","DOI":"10.3390\/molecules22061003","type":"journal-article","created":{"date-parts":[[2017,6,16]],"date-time":"2017-06-16T10:06:31Z","timestamp":1497607591000},"page":"1003","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Anti-Inflammatory and Anti-Oxidant Potential of the Root Extract and Constituents of Doronicum austriacum"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2333-1630","authenticated-orcid":false,"given":"Stefania","family":"Marzocco","sequence":"first","affiliation":[{"name":"Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano SA, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Adesso","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano SA, Italy"},{"name":"PhD Program in Drug Discovery and Development, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano SA, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa","family":"Alilou","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Member of the CMBI, University of Innsbruck, CCB, Innrain 80-82, 6020 Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Stuppner","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Member of the CMBI, University of Innsbruck, CCB, Innrain 80-82, 6020 Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3731-7349","authenticated-orcid":false,"given":"Stefan","family":"Schwaiger","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Pharmacognosy, Member of the CMBI, University of Innsbruck, CCB, Innrain 80-82, 6020 Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,6,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1146\/annurev.immunol.15.1.323","article-title":"Nitric oxide and macrophage function","volume":"15","author":"MacMicking","year":"1997","journal-title":"Annu. Rev. Immunol."},{"key":"ref_2","first-page":"117","article-title":"Effect of vitamin and trace element supplementation on immune indices in healthy elderly","volume":"65","author":"Pike","year":"1995","journal-title":"Int. J. Vitam. Nutr. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"346","DOI":"10.3109\/10715762.2013.779373","article-title":"Oxidative stress in patients with cardiovascular disease and chronic renal failure","volume":"47","author":"Popolo","year":"2013","journal-title":"Free Radic. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5900","DOI":"10.1523\/JNEUROSCI.22-14-05900.2002","article-title":"Inflammation-dependent cerebral deposition of serum amyloid a protein in a mouse model of amyloidosis","volume":"22","author":"Guo","year":"2002","journal-title":"J. Neurosci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/1750-1326-4-47","article-title":"Does neuroinflammation fan the flame in neurodegenerative diseases?","volume":"4","author":"Alto","year":"2009","journal-title":"Mol. Neurodegener."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0891-5849(91)90009-R","article-title":"Oxygen free radicals and Parkinson\u2019s disease","volume":"10","author":"Adams","year":"1991","journal-title":"Free Radic. Biol. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/j.1749-6632.1994.tb21836.x","article-title":"Advanced Maillard reaction end products, free radicals, and protein oxidation in Alzheimer\u2019s disease","volume":"738","author":"Smith","year":"1994","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/exnr.1998.7006","article-title":"Recent advances on the pathogenesis of Huntington\u2019s disease","volume":"157","author":"Petersen","year":"1999","journal-title":"Exp. Neurol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"65","DOI":"10.2174\/157015909787602823","article-title":"Oxidative Stress and Neurodegenerative Diseases: A Review of Upstream and Downstream Antioxidant Therapeutic Options","volume":"7","author":"Uttara","year":"2009","journal-title":"Curr. Neuropharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/S0891-5849(96)00432-7","article-title":"The origin of the hydroxyl radical oxygen in the Fenton Reaction","volume":"22","author":"Lloyd","year":"1997","journal-title":"Free Radic. Biol. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"484613","DOI":"10.1155\/2013\/484613","article-title":"Role of redox signaling in neuroinflammation and neurodegenerative diseases","volume":"2013","author":"Hsieh","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3575","DOI":"10.1016\/S0040-4039(01)98531-4","article-title":"New Benzofuran Derivatives from Doronicum austriacum Jacq","volume":"41","author":"Bohlmann","year":"1970","journal-title":"Tetrahedron Lett."},{"key":"ref_13","unstructured":"Tabernaemontanus, J.T. (2017, February 16). New Vollkommen Kr\u00e4uter-Buch, 1625. Available online: http:\/\/www.kraeuter.ch."},{"key":"ref_14","first-page":"169","article-title":"Alkaloid concentrate from Doronicum austriacum inhibits the growth of mouse fibroblasts in vitro and the growth of mouse mammary carcinoma in vivo","volume":"41","author":"Osmak","year":"1991","journal-title":"Acta Pharm. Jugosl."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/S0031-9422(00)84289-2","article-title":"Ein neuer Sesquiterpenalkohol und andere Inhaltsstoffe aus Doronicum pardalianches","volume":"18","author":"Bohlmann","year":"1979","journal-title":"Phytochemistry"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0031-9422(00)90944-0","article-title":"Neue Tremeton-derivate aus Doronicum macrophyllum","volume":"18","author":"Bohlmann","year":"1979","journal-title":"Phytochemistry"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2857","DOI":"10.1021\/jo062447h","article-title":"Stereoselective syntheses of the 2-Isopropenyl-2,3-dihydrobenzofuran nucleus: Potential chiral building blocks for the syntheses of tremetone, hydroxytremetone, and rotenone","volume":"72","author":"Pelly","year":"2007","journal-title":"J. Org. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"281","DOI":"10.31887\/DCNS.2009.11.3\/mbelanger","article-title":"The role of astroglia in neuroprotection","volume":"11","author":"Magistretti","year":"2009","journal-title":"Dialogues Clin. Neurosci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.tibs.2003.10.006","article-title":"NO-dependent protein nitration: A cell signaling event or an oxidative inflammatory response?","volume":"28","author":"Schopfer","year":"2003","journal-title":"Trends Biochem. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2092","DOI":"10.1021\/jf505614z","article-title":"Toxicity of white snake-root (Ageratina altissima) and chemical extracts of white snake-root in goats","volume":"63","author":"Davis","year":"2015","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1021\/jm00339a023","article-title":"The synthesis and pharmacological action of tremetone","volume":"6","author":"Bowen","year":"1963","journal-title":"J. Med. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.foodchem.2014.06.105","article-title":"Evaluation of anti-inflammatory activity and fast UHPLC-DAD-IT-TOF profiling of polyphenolic compounds extracted from green lettuce (Lactuca sativa L. var. Maravilla de Verano)","volume":"167","author":"Pepe","year":"2015","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"370","DOI":"10.3389\/fphar.2017.00370","article-title":"Indoxyl sulfate affects glial function increasing oxidative stress and neuroinflammation in chronic kidney disease: Interaction between astrocytes and microglia","volume":"8","author":"Adesso","year":"2017","journal-title":"Front. Pharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.toxicon.2012.02.010","article-title":"Modulation of macrophage activity by aflatoxins B1 and B2 and their metabolites aflatoxins M1 and M2","volume":"59","author":"Bianco","year":"2012","journal-title":"Toxicon"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.taap.2015.04.002","article-title":"Nivalenol induces oxidative stress and increases deoxynivalenol pro-oxidant effect in intestinal epithelial cells","volume":"285","author":"Adesso","year":"2015","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3778","DOI":"10.1039\/C5FO00475F","article-title":"Anti-inflammatory activity of horseradish (Armoracia rusticana) root extracts in LPS-stimulated macrophages","volume":"6","author":"Marzocco","year":"2015","journal-title":"Food Funct."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1002\/chir.22138","article-title":"SpecDis: Quantifying the comparison of calculated and experimental electronic circular dichroism spectra","volume":"25","author":"Bruhn","year":"2013","journal-title":"Chirality"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/6\/1003\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:39:21Z","timestamp":1760207961000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/6\/1003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,16]]},"references-count":27,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2017,6]]}},"alternative-id":["molecules22061003"],"URL":"https:\/\/doi.org\/10.3390\/molecules22061003","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,16]]}}}