{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:00:17Z","timestamp":1790787617043,"version":"4.1.0"},"reference-count":32,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2017,6,27]],"date-time":"2017-06-27T00:00:00Z","timestamp":1498521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Leishmania mexicana is one of the pathogens causing cutaneous leishmaniasis which is associated with patient morbidity. In our researches for new safe and effective treatments, thirty-seven essential oils (EOs) extracted from Vietnamese plants were screened in vitro for the first time on Leishmania mexicana mexicana (Lmm) promastigotes at the maximum concentration of 50 nL\/mL. Active EOs were also analyzed for cytotoxicity on mammalian cell lines (WI38, J774) and their selectivity indices (SI) were calculated. Their composition was determined by GC-MS and GC-FID. Our results indicated that EOs extracted from Cinnamomum cassia, Zingiber zerumbet, Elsholtzia ciliata and Amomum aromaticum, possessed a moderate anti-leishmanial activity, with IC50 values of 2.92 \u00b1 0.08, 3.34 \u00b1 0.34, 8.49 \u00b1 0.32 and 9.25 \u00b1 0.64 nL\/mL respectively. However, they also showed cytotoxicity with SI &lt; 10. The most promising EO was extracted from Ocimum gratissimum, displaying an IC50 of 4.85 \u00b1 1.65 nL\/mL and SI &gt; 10. It contained 86.5% eugenol, which was demonstrated to be effective on Lmm with IC50 of 2.57 \u00b1 0.57 nL\/mL and not toxic on mammalian cells, explaining the observed activity.<\/jats:p>","DOI":"10.3390\/molecules22071071","type":"journal-article","created":{"date-parts":[[2017,6,28]],"date-time":"2017-06-28T10:25:56Z","timestamp":1498645556000},"page":"1071","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["In Vitro Anti-Leishmanial Activity of Essential Oils Extracted from Vietnamese Plants"],"prefix":"10.3390","volume":"22","author":[{"given":"Thanh Binh","family":"Le","sequence":"first","affiliation":[{"name":"GNOS Research Group, Louvain Drug Research Institue, Universit\u00e9 Catholique de Louvain, Bruxelles 1200, Belgium"},{"name":"Department of Pharmacognosy, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hoan Kiem, Hanoi 100000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0942-4649","authenticated-orcid":false,"given":"Claire","family":"Beaufay","sequence":"additional","affiliation":[{"name":"GNOS Research Group, Louvain Drug Research Institue, Universit\u00e9 Catholique de Louvain, Bruxelles 1200, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2381-6144","authenticated-orcid":false,"given":"Duc Trong","family":"Nghiem","sequence":"additional","affiliation":[{"name":"Department of Botany, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hoan Kiem, Hanoi 100000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Paule","family":"Mingeot-Leclercq","sequence":"additional","affiliation":[{"name":"TFAR Research Group, Louvain Drug Research Institue, Universit\u00e9 Catholique de Louvain, Bruxelles 1200, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2278-6206","authenticated-orcid":false,"given":"Jo\u00eblle","family":"Quetin-Leclercq","sequence":"additional","affiliation":[{"name":"GNOS Research Group, Louvain Drug Research Institue, Universit\u00e9 Catholique de Louvain, Bruxelles 1200, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,6,27]]},"reference":[{"key":"ref_1","unstructured":"Centers for Disease Control and Prevention (CDC) (2017, June 08). Parasites-Leishmaniasis, Available online: https:\/\/www.cdc.gov\/parasites\/leishmaniasis\/."},{"key":"ref_2","unstructured":"WHO Expert Committee (2010). Control of the Leishmaniases, World Health Organization."},{"key":"ref_3","unstructured":"Drugs for Neglected Diseases initiative (DNDi) (2017, June 08). Diseases & Projects-Leishmaniasis. Available online: http:\/\/www.dndi.org\/diseases-projects\/leishmaniasis\/."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2128","DOI":"10.2174\/092986712800229023","article-title":"The potential of secondary metabolites from plants as drugs or leads against protozoan neglected diseases-part I","volume":"19","author":"Schmidt","year":"2012","journal-title":"Curr. Med. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.fct.2007.09.106","article-title":"Biological effects of essential oils\u2014A review","volume":"46","author":"Bakkali","year":"2008","journal-title":"Food. Chem. Toxicol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.exppara.2013.10.007","article-title":"Essential oil from Chenopodium ambrosioides and main components: Activity against Leishmania, their mitochondria and other microorganisms","volume":"136","author":"Monzote","year":"2014","journal-title":"Exp. Parasitol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/j.phymed.2014.03.002","article-title":"Antileishmanial activity of essential oil from Chenopodium ambrosioides and its main components against experimental cutaneous leishmaniasis in BALB\/c mice","volume":"21","author":"Monzote","year":"2014","journal-title":"Phytomedicine"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1002\/ptr.5055","article-title":"Antileishmanial activity of the essential oil from Bixa orellana","volume":"28","author":"Monzote","year":"2014","journal-title":"Phytother. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Bagetta, G., Cosentino, M., and Sakurada, T. (2015). Extraction, sample preparation, and analytical methods for quality issues of essential oils. Aromatherapy: Basic Mechanisms and Evidence-Based Clinical Use, CRC Press.","DOI":"10.1201\/b19651"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1016\/j.jpba.2006.09.005","article-title":"Determination of new retention indices for quick identification of essential oils compounds","volume":"43","author":"Tilquin","year":"2007","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_11","unstructured":"Gurib-Fakim, A. (2014). Anti-parasitic activity of essential oils and their constituents against Plasmodium, Trypanosoma and Leishmania. Novel Plant Bioresource: Applications in Food, Medicine and Cosmetic, John Wiley & Sons."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1089\/jmf.2015.3475","article-title":"Essential Oils from the Medicinal Herbs Upregulate Dopamine Transporter in Rat Pheochromocytoma Cells","volume":"18","author":"Choi","year":"2015","journal-title":"J. Med. Food."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.parint.2005.10.006","article-title":"Antileishmanial activity of Eugenol-rich essential oil from Ocimum gratissimum","volume":"55","author":"Alviano","year":"2006","journal-title":"Parasitol. Int."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1016\/j.jep.2014.07.014","article-title":"In vitro antitrypanosomal and antiplasmodial activities of crude extracts and essential oils of Ocimum gratissimum Linn from Benin and influence of vegetative stage","volume":"155","author":"Kpoviessi","year":"2014","journal-title":"J. Ethnopharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1007\/s00436-008-1288-6","article-title":"Effects of essential oils from Cymbopogon citratus (DC) Stapf., Lippia sidoides Cham., and Ocimum gratissimum L. on growth and ultrastructure of Leishmania chagasi promastigotes","volume":"104","author":"Oliveira","year":"2009","journal-title":"Parasitol. Res."},{"key":"ref_16","first-page":"130","article-title":"Chemical composition and biological activity of the essential oil of rhizome of Zingiber zerumbet (L.) Smith","volume":"3","author":"Singh","year":"2014","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1080\/10412905.2013.820669","article-title":"Antiprotozoal activity of essential oils derived from Piper spp. grown in Colombia","volume":"25","author":"Leal","year":"2013","journal-title":"J. Essent. Oil Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.vetpar.2013.11.016","article-title":"Activity of Thymus capitellatus volatile extract, 1,8-cineole and borneol against Leishmania species","volume":"200","author":"Machado","year":"2014","journal-title":"Vet. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.tiv.2013.10.006","article-title":"Identification of toxicants in cinnamon-flavored electronic cigarette refill fluids","volume":"28","author":"Behar","year":"2014","journal-title":"Toxicol. In Vitro"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1021\/jf0725629","article-title":"Content, composition, and bioactivity of the essential oils of three basil genotypes as a function of harvesting","volume":"56","author":"Zheljazkov","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1007\/s00436-009-1578-7","article-title":"In vitro activity of the essential oil of Cymbopogon citratus and its major component (citral) on Leishmania amazonensis","volume":"105","author":"Santin","year":"2009","journal-title":"Parasitol. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.exppara.2011.12.012","article-title":"Monoterpenic aldehydes as potential anti-Leishmania agents: Activity of Cymbopogon citratus and citral on L. infantum, L. tropica and L. major","volume":"130","author":"Machado","year":"2012","journal-title":"Exp. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Xavier, F.J.S., Rodrigues, K.A.D.F., de Oliveira, R.G., Lima Junior, C.G., Rocha, J.D., Keesen, T.S., de Oliveira, M.R., Silva, F.P., and Vasconcellos, M.L. (2016). Synthesis and in vitro anti Leishmania amazonensis biological screening of Morita-Baylis-Hillman Adducts prepared from eugenol, thymol and carvacrol. Molecules, 21.","DOI":"10.3390\/molecules21111483"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.actatropica.2016.08.026","article-title":"Effects of linalool and eugenol on the survival of Leishmania (L.) infantum chagasi within macrophages","volume":"164","author":"Dutra","year":"2016","journal-title":"Acta Trop."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6250","DOI":"10.1016\/j.bmc.2014.08.020","article-title":"Thymol and eugenol derivatives as potential antileishmanial agents","volume":"22","author":"Bevilaqua","year":"2014","journal-title":"Bioorg. Med. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Islamuddin, M., Chouhan, G., Want, M.Y., Ozbak, H.A., Hemeg, H.A., and Afrin, F. (2016). Immunotherapeutic potential of eugenol emulsion in experimental visceral leishmaniasis. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0005011"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.bjid.2015.10.002","article-title":"Induction of apoptosis by zerumbone isolated from Zingiber zerumbet (L.) Smith in protozoan parasite Leishmania donovani due to oxidative stress","volume":"20","author":"Mukherjee","year":"2016","journal-title":"Braz. J. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1055\/s-2005-837820","article-title":"Antitumor effects of zerumbone from Zingiber zerumbet in P-388D1 cells in vitro and in vivo","volume":"71","author":"Huang","year":"2005","journal-title":"Planta Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1055\/s-0034-1383183","article-title":"Pharmacological activities of cilantro\u2019s aliphatic aldehydes against Leishmania donovani","volume":"80","author":"Donega","year":"2014","journal-title":"Planta Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jep.2014.11.024","article-title":"Syzygium cumini (L.) Skeels essential oil and its major constituent \u03b1-pinene exhibit anti-Leishmania activity through immunomodulation in vitro","volume":"160","author":"Rodrigues","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6953","DOI":"10.3390\/molecules17066953","article-title":"Eugenol-From the remote Maluku Islands to the international market place: A review of a remarkable and versatile molecule","volume":"17","author":"Kamatou","year":"2012","journal-title":"Molecules"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/2191-0855-3-54","article-title":"Phenyl aldehyde and propanoids exert multiple sites of action towards cell membrane and cell wall targeting ergosterol in Candida albicans","volume":"3","author":"Khan","year":"2013","journal-title":"AMB Express"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/7\/1071\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:40:35Z","timestamp":1760208035000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/7\/1071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,27]]},"references-count":32,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2017,7]]}},"alternative-id":["molecules22071071"],"URL":"https:\/\/doi.org\/10.3390\/molecules22071071","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,27]]}}}