{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:30:26Z","timestamp":1761611426152,"version":"build-2065373602"},"reference-count":29,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2017,7,21]],"date-time":"2017-07-21T00:00:00Z","timestamp":1500595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Henan Province University Science and Technology Innovation Team","award":["(16IRTSTHN019)"],"award-info":[{"award-number":["(16IRTSTHN019)"]}]},{"name":"Science and Technology Innovation Team of Kaifeng City","award":["(2016-124)"],"award-info":[{"award-number":["(2016-124)"]}]},{"name":"Key Project in Science and Technology Agency of Kaifeng City","award":["(1608003)"],"award-info":[{"award-number":["(1608003)"]}]},{"name":"Kaifeng City Science and Technology Innovation Talent","award":["(1509010)"],"award-info":[{"award-number":["(1509010)"]}]},{"name":"Key Project in Science and Technology Agency of Henan Province","award":["(172102310609)"],"award-info":[{"award-number":["(172102310609)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Different extracts of Angelica dahuricae were available for whitening or treating vitiligo clinically. They showed inhibitory or activating effects on tyrosinase, a rate-limiting enzyme of melanogenesis. This study aimed to identify active compounds on tyrosinase in water extract of Angelica dahurica Radix. We applied spectrum-effect relationship and component knock-out methods to make it clear. HPLC was used to obtain the specific chromatograms. The effects on tyrosinase activity were examined by measuring the oxidation rate of levodopa in vitro. Partial least squares method was used to examine the spectrum-effect relationships. The knocked-out samples were prepared by HPLC method, and the identification of knocked-out compounds was conducted by the high performance liquid chromatography-four stage rod-electrostatic field orbit trap high resolution mass spectrometry. Results showed that S6, S14, S18, S21, S35, S36, S37, S40, and S41 were positively correlated to inhibitory activity of Angelica dahuricae on tyrosinase whereas S9, S11, S8, S12, S22, and S30 were negatively correlated. When the concentration of each sample was 1 g\u00b7mL\u22121, equal to the amount of raw medicinal herbs, oxypeucedanin hydrate, imperatorin, cnidilin, and isoimperatorin had inhibitory effects on tyrosinase activity whereas byakangelicin and bergapten had activating effects.<\/jats:p>","DOI":"10.3390\/molecules22071231","type":"journal-article","created":{"date-parts":[[2017,7,21]],"date-time":"2017-07-21T10:45:48Z","timestamp":1500633948000},"page":"1231","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Spectrum Effect Relationship and Component Knock-Out in Angelica Dahurica Radix by High Performance Liquid Chromatography-Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer"],"prefix":"10.3390","volume":"22","author":[{"given":"Jinmei","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China"},{"name":"Kaifeng Key Laboratory of Functional Components in Health Food, Henan University, Kaifeng, Henan 475004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linna","family":"Peng","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengjun","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changqin","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hebei Food Inspection and Research Institute, Shijiazhuang 050091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1822-6249","authenticated-orcid":false,"given":"Wenyi","family":"Kang","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China"},{"name":"Kaifeng Key Laboratory of Functional Components in Health Food, Henan University, Kaifeng, Henan 475004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,7,21]]},"reference":[{"key":"ref_1","unstructured":"Chinese Pharmacopoeia Commission (2015). 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Form"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/7\/1231\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:43:38Z","timestamp":1760208218000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/7\/1231"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,21]]},"references-count":29,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2017,7]]}},"alternative-id":["molecules22071231"],"URL":"https:\/\/doi.org\/10.3390\/molecules22071231","relation":{},"ISSN":["1420-3049"],"issn-type":[{"type":"electronic","value":"1420-3049"}],"subject":[],"published":{"date-parts":[[2017,7,21]]}}}