{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T18:05:42Z","timestamp":1789927542685,"version":"4.0.1"},"reference-count":74,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Protein S-sulfhydration is a newly discovered post-translational modification of specific cysteine residue(s) in target proteins, which is involved in a broad range of cellular functions and metabolic pathways. By changing local conformation and the final activity of target proteins, S-sulfhydration is believed to mediate most cellular responses initiated by H2S, a novel gasotransmitter. In comparison to protein S-sulfhydration, nitric oxide-mediated protein S-nitrosylation has been extensively investigated, including its formation, regulation, transfer and metabolism. Although the investigation on the regulatory mechanisms associated with protein S-sulfhydration is still in its infancy, accumulated evidence suggested that protein S-sulfhydration may share similar chemical features with protein S-nitrosylation. Glutathione persulfide acts as a major donor for protein S-sulfhydration. Here, we review the present knowledge on protein S-sulfhydration, and also predict its formation and regulation mechanisms based on the knowledge from protein S-nitrosylation.<\/jats:p>","DOI":"10.3390\/molecules22081334","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T10:07:40Z","timestamp":1502446060000},"page":"1334","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["H2S-Mediated Protein S-Sulfhydration: A Prediction for Its Formation and Regulation"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8325-1494","authenticated-orcid":false,"given":"Youngjun","family":"Ju","sequence":"first","affiliation":[{"name":"School of Kinesiology, Lakehead University, Thunder Bay, ON P7B 5E1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Fu","sequence":"additional","affiliation":[{"name":"Cardiovascular and Metabolic Research Unit, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada"},{"name":"School of Human Kinetics, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada"},{"name":"Health Science North Research Institute, 41 Ramsey Lake Rd, Sudbury, ON P3E 5J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Stokes","sequence":"additional","affiliation":[{"name":"Cardiovascular and Metabolic Research Unit, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada"},{"name":"The Department of Biology, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Wu","sequence":"additional","affiliation":[{"name":"Cardiovascular and Metabolic Research Unit, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada"},{"name":"School of Human Kinetics, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada"},{"name":"Health Science North Research Institute, 41 Ramsey Lake Rd, Sudbury, ON P3E 5J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangdong","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Kinesiology, Lakehead University, Thunder Bay, ON P7B 5E1, Canada"},{"name":"Cardiovascular and Metabolic Research Unit, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada"},{"name":"The Department of Chemistry and Biochemistry, Laurentian University, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jaffrey, S.R., and Snyder, S.H. 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