{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:23:14Z","timestamp":1787505794930,"version":"build-2736575974"},"reference-count":66,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T00:00:00Z","timestamp":1505347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Schistosomiasis, which is caused by helminth trematode blood flukes of the genus Schistosoma, is a serious health and economic problem in tropical areas, and the second most prevalent parasitic disease after malaria. Currently, there is no effective vaccine available and treatment is entirely dependent on a single drug, praziquantel (PZQ), raising a significant potential public health threat due to the emergence of PZQ drug resistance. It is thus urgent and necessary to explore novel therapeutic targets for the treatment of schistosomiasis. Previous studies demonstrated that acetylcholinesterase (AChE) and nicotinic acetylcholine receptors (nAChRs) play important roles in the schistosome nervous system and ion channels, both of which are targeted by a number of currently approved and marketed anthelminthic drugs. To improve understanding of the functions of the cholinergic system in schistosomes, this article reviews previous studies on AChE and nAChRs in schistosomes and other helminths and discusses their potential as suitable targets for vaccine development and drug design against schistosomiasis.<\/jats:p>","DOI":"10.3390\/molecules22091550","type":"journal-article","created":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T10:04:49Z","timestamp":1505383489000},"page":"1550","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Acetylcholinesterase and Nicotinic Acetylcholine Receptors in Schistosomes and Other Parasitic Helminths"],"prefix":"10.3390","volume":"22","author":[{"given":"Hong","family":"You","sequence":"first","affiliation":[{"name":"Molecular Parasitology Laboratory, Infectious Diseases Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland 4006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Laboratory, Infectious Diseases Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland 4006, Australia"},{"name":"Parasitology Laboratory, School of Animal Medicine, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Du","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Laboratory, Infectious Diseases Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland 4006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald","family":"McManus","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Laboratory, Infectious Diseases Division, QIMR Berghofer Medical Research Institute, Brisbane, Queensland 4006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,9,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.trsl.2013.03.006","article-title":"New vaccines for neglected parasitic diseases and dengue","volume":"162","author":"Beaumier","year":"2013","journal-title":"Transl. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s10158-007-0059-x","article-title":"Ion-channels on parasite muscle: Pharmacology and physiology","volume":"7","author":"Robertson","year":"2007","journal-title":"Invert. Neurosci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1007\/s00436-008-1080-7","article-title":"Identification of the amino-acetonitrile derivative monepantel (AAD 1566) as a new anthelmintic drug development candidate","volume":"103","author":"Kaminsky","year":"2008","journal-title":"Parasitol. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1111\/j.1476-5381.1972.tb08145.x","article-title":"Inhibition by metrifonate and dichlorvos of cholinesterases in schistosomes","volume":"46","author":"Bueding","year":"1972","journal-title":"Br. J. Pharmacol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1017\/S003118200006193X","article-title":"Schistosoma mansoni: A scanning electron microscope study of the developing schistosomulum","volume":"81","author":"Crabtree","year":"1980","journal-title":"Parasitology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.jare.2012.05.002","article-title":"Novel therapeutic and prevention approaches for schistosomiasis: Review","volume":"4","author":"Tallima","year":"2013","journal-title":"J. Adv. Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mahmoud, A.A.F. (2001). Strategies for Control of Infection and Disease: Current Practice and Future Potential. Schistosomiasis, Imperial College Press.","DOI":"10.1142\/p126"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/0014-4894(88)90014-8","article-title":"Schistosoma mansoni: Analysis of cercarial transformation methods","volume":"67","author":"Salafsky","year":"1988","journal-title":"Exp. Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1096\/fj.01-0683fje","article-title":"Molecular characterization of an acetylcholinesterase implicated in the regulation of glucose scavenging by the parasite Schistosoma","volume":"16","author":"Jones","year":"2002","journal-title":"FASEB J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1017\/S0033583510000168","article-title":"The structural basis of function in Cys-loop receptors","volume":"43","author":"Thompson","year":"2010","journal-title":"Q. Rev. Biophys."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1021\/cr00081a005","article-title":"Acetylcholinesterase: Enzyme structure, reaction dynamics and virtual transition states","volume":"87","author":"Quinn","year":"1987","journal-title":"Chem. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.ijpddr.2011.09.003","article-title":"Ion channels and receptor as targets for the control of parasitic nematodes","volume":"1","author":"Wolstenholme","year":"2011","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/0005-2736(93)90328-W","article-title":"The voltage-gated non-selective cation channel from human red cells is sensitive to acetylcholine","volume":"1147","author":"Bennekou","year":"1993","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_14","first-page":"656","article-title":"The possible role of acetylcholine in Schistosoma mansoni","volume":"26","author":"Barker","year":"1966","journal-title":"Br. J. Pharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1017\/S0031182000066270","article-title":"Cholinergic inhibition of muscle fibres isolated from Schistosoma mansoni (Trematoda:Digenea)","volume":"113","author":"Day","year":"1996","journal-title":"Parasitology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1002\/iub.170","article-title":"Recent advances in understanding the structure of nicotinic acetylcholine receptors","volume":"61","author":"Zouridakis","year":"2009","journal-title":"IUBMB Life"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1097\/00000542-200201000-00035","article-title":"Advances in neurobiology of the neuromuscular junction: Implications for the anesthesiologist","volume":"96","author":"Naguib","year":"2002","journal-title":"Anesthesiology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1002\/bies.201300082","article-title":"Are nicotinic acetylcholine receptors coupled to G proteins?","volume":"35","author":"Kabbani","year":"2013","journal-title":"Bioessays"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.gene.2003.12.009","article-title":"ShAR1alpha and ShAR1beta: Novel putative nicotinic acetylcholine receptor subunits from the platyhelminth blood fluke Schistosoma","volume":"329","author":"Bentley","year":"2004","journal-title":"Gene"},{"key":"ref_20","first-page":"563","article-title":"Acetylcholinesterase activity of Schistosoma mansoni","volume":"7","author":"Bueding","year":"1952","journal-title":"Br. J. Pharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"S83","DOI":"10.1017\/S003118200007791X","article-title":"Neuromuscular physiology and pharmacology of parasitic flatworms","volume":"113","author":"Pax","year":"1996","journal-title":"Parasitology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0165-2478(91)90116-R","article-title":"Acetylcholinesterase from Schistosoma mansoni: Immunological characterization","volume":"28","author":"Espinoza","year":"1991","journal-title":"Immunol. Lett."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.molbiopara.2005.01.019","article-title":"Expression and comparative functional characterisation of recombinant acetylcholinesterase from three species of Schistosoma","volume":"141","author":"Bentley","year":"2005","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1186\/s13071-016-1615-1","article-title":"Functional characterisation of Schistosoma japonicum acetylcholinesterase","volume":"9","author":"You","year":"2016","journal-title":"Parasit. Vectors"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0166-6851(84)90120-8","article-title":"Isolation and partial characterization of the tegumental outer membrane of schistosomula of Schistosoma mansoni","volume":"13","author":"Schryer","year":"1984","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3389\/fimmu.2015.00213","article-title":"Of monkeys and men: Immunomic profiling of sera from humans and non-human primates resistant to schistosomiasis reveals novel potential vaccine candidates","volume":"6","author":"Pearson","year":"2015","journal-title":"Front. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.1110\/ps.8.12.2553","article-title":"Acetylcholinesterase of Schistosoma mansoni\u2014Functional correlates. Contributed in honor of Professor Hans Neurath\u2019s 90th birthday","volume":"8","author":"Arnon","year":"1999","journal-title":"Protein Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1089\/hyb.1995.14.577","article-title":"Monoclonal antibodies against acetylcholinesterase of Schistosoma mansoni: Production and characterization","volume":"14","author":"Espinoza","year":"1995","journal-title":"Hybridoma"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1006\/expr.1995.1152","article-title":"Schistosoma: Rate of glucose import is altered by acetylcholine interaction with tegumental acetylcholine receptors and acetylcholinesterase","volume":"81","author":"Camacho","year":"1995","journal-title":"Exp. Parasitol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1007\/s00436-006-0175-2","article-title":"Schistosomiasis\u2014A century searching for chemotherapeutic drugs","volume":"99","author":"Leite","year":"2006","journal-title":"Parasitol. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1017\/S0031182000068244","article-title":"The amount of acetylcholinesterase on the parasite surface reflects the differential sensitivity of schistosome species to metrifonate","volume":"108","author":"Camacho","year":"1994","journal-title":"Parasitology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s00436-001-0587-y","article-title":"Chemotherapeutic approaches to schistosomes: Current knowledge and outlook","volume":"88","author":"Harder","year":"2002","journal-title":"Parasitol. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/0020-7519(96)00040-9","article-title":"Why do some nematode parasites of the alimentary tract secrete acetylcholinesterase?","volume":"26","author":"Lee","year":"1996","journal-title":"Int. J. Parasitol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1038\/nature08160","article-title":"The genome of the blood fluke Schistosoma mansoni","volume":"460","author":"Berriman","year":"2009","journal-title":"Nature"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1038\/ng.1065","article-title":"Whole-genome sequence of Schistosoma haematobium","volume":"44","author":"Young","year":"2012","journal-title":"Nat. Genet."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Schistosoma japonicum Genome Sequencing and Functional Analysis Consortium (2009). The Schistosoma japonicum genome reveals features of host-parasite interplay. Nature, 460, 345\u2013351.","DOI":"10.1038\/nature08140"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1146\/annurev.ne.05.030182.000421","article-title":"The molecular forms of cholinesterase and acetylcholinesterase in vertebrates","volume":"5","author":"Massoulie","year":"1982","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1042\/bj3440945","article-title":"Acetylcholinesterase from Schistosoma mansoni: Interaction of globular species with heparin","volume":"344","author":"Toker","year":"1999","journal-title":"Biochem. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0167-4838(84)90332-7","article-title":"Acetylcholinesterase of Schistosoma mansoni. Molecular forms of the solubilized enzyme","volume":"790","author":"Gonzales","year":"1984","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1111\/j.1432-1033.1991.tb15776.x","article-title":"Phosphatidylinositol-specific phospholipase C induces biosynthesis of acetylcholinesterase via diacylglycerol in Schistosoma mansoni","volume":"195","author":"Espinoza","year":"1991","journal-title":"Eur. J. Biochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/0166-6851(88)90072-2","article-title":"Acetylcholinesterase in Schistosoma mansoni is anchored to the membrane via covalently attached phosphatidylinositol","volume":"29","author":"Espinoza","year":"1988","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"MacDonald, K., Buxton, S., Kimber, M.J., Day, T.A., Robertson, A.P., and Ribeiro, P. (2014). Functional characterization of a novel family of acetylcholine-gated chloride channels in Schistosoma mansoni. PLoS Pathog., 10.","DOI":"10.1371\/journal.ppat.1004181"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0166-6851(94)00039-P","article-title":"Nicotinic acetylcholine receptors on the surface of the blood fluke Schistosoma","volume":"71","author":"Camacho","year":"1995","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1017\/S0031182006002162","article-title":"ShAR2beta, a divergent nicotinic acetylcholine receptor subunit from the blood fluke Schistosoma","volume":"134","author":"Bentley","year":"2007","journal-title":"Parasitology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"637","DOI":"10.2307\/3283866","article-title":"Glucose uptake rates by Schistosoma mansoni, S. haematobium, and S. bovis adults using a flow in vitro culture system","volume":"81","author":"Camacho","year":"1995","journal-title":"J. Parasitol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/bs.irn.2015.07.001","article-title":"Neuronal Nicotinic Acetylcholine receptor structure and function and response to nicotine","volume":"124","author":"Dani","year":"2015","journal-title":"Int. Rev. Neurobiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.parint.2013.03.010","article-title":"Characterization of cys-loop receptor genes involved in inhibitory amine neurotransmission in parasitic and free living nematodes","volume":"62","author":"Beech","year":"2013","journal-title":"Parasitol. Int."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Mansour, T.E. (2002). Chemotherapeutic Targets in Parasites Contemporary Strategies, Cambridge University Press.","DOI":"10.1017\/CBO9780511546440"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"858","DOI":"10.2307\/3281834","article-title":"Effects of cholinergic drugs on longitudinal muscle contractions of Fasciola hepatica","volume":"72","author":"Sukhdeo","year":"1986","journal-title":"J. Parasitol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1017\/S0031182000064180","article-title":"Acetylcholinesterase secretion\u2014A parameter for the interpretation of in vitro anthelmintic screens","volume":"92","author":"Rapson","year":"1986","journal-title":"Parasitology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/0020-7519(73)90083-0","article-title":"Acetylcholinesterase secretion by parasitic nematodes\u2014I. Evidence for secretion of the enzyme by a number of species","volume":"3","author":"Ogilvie","year":"1973","journal-title":"Int. J. Parasitol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Vaux, R., Schnoeller, C., Berkachy, R., Roberts, L.B., Hagen, J., Gounaris, K., and Selkirk, M.E. (2016). Modulation of the Immune Response by Nematode Secreted Acetylcholinesterase Revealed by Heterologous Expression in Trypanosoma musculi. PLoS Pathog., 12.","DOI":"10.1371\/journal.ppat.1005998"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.2307\/3282227","article-title":"Histochemical localization of acetylcholinesterase in the cerebral ganglia of Fasciola hepatica, a parasitic flatworm","volume":"74","author":"Sukhdeo","year":"1988","journal-title":"J. Parasitol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1002\/jcp.20495","article-title":"Localization, specific activity, and molecular forms of acetylcholinesterase in developmental stages of the cestode Mesocestoides corti","volume":"206","author":"Kemmerling","year":"2006","journal-title":"J. Cell Physiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S1090-0233(05)80005-X","article-title":"Modes of action of anthelmintic drugs","volume":"154","author":"Martin","year":"1997","journal-title":"Vet. J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1002\/bies.10377","article-title":"Functional genomics of the nicotinic acetylcholine receptor gene family of the nematode, Caenorhabditis elegans","volume":"26","author":"Jones","year":"2004","journal-title":"Bioessays"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/12160","article-title":"One GABA and two acetylcholine receptors function at the C. elegans neuromuscular junction","volume":"2","author":"Richmond","year":"1999","journal-title":"Nat. Neurosci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"27013","DOI":"10.1074\/jbc.M502818200","article-title":"acr-16 encodes an essential subunit of the levamisole-resistant nicotinic receptor at the Caenorhabditis elegans neuromuscular junction","volume":"280","author":"Touroutine","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"18590","DOI":"10.1073\/pnas.0806933105","article-title":"Eight genes are required for functional reconstitution of the Caenorhabditis elegans levamisole-sensitive acetylcholine receptor","volume":"105","author":"Boulin","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Buxton, S.K., Charvet, C.L., Neveu, C., Cabaret, J., Cortet, J., Peineau, N., Abongwa, M., Courtot, E., Robertson, A.P., and Martin, R.J. (2014). Investigation of acetylcholine receptor diversity in a nematode parasite leads to characterization of tribendimidine- and derquantel-sensitive nAChRs. PLoS Pathog., 10.","DOI":"10.1371\/journal.ppat.1003870"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Duguet, T.B., Charvet, C.L., Forrester, S.G., Wever, C.M., Dent, J.A., Neveu, C., and Beech, R.N. (2016). Recent Duplication and Functional Divergence in Parasitic Nematode Levamisole-Sensitive Acetylcholine Receptors. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004826"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Williamson, S.M., Robertson, A.P., Brown, L., Williams, T., Woods, D.J., Martin, R.J., Sattelle, D.B., and Wolstenholme, A.J. (2009). The nicotinic acetylcholine receptors of the parasitic nematode Ascaris suum: Formation of two distinct drug targets by varying the relative expression levels of two subunits. PLoS Pathog., 5.","DOI":"10.1371\/journal.ppat.1000517"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1016\/j.ijpara.2004.04.014","article-title":"Oxantel is an N-type (methyridine and nicotine) agonist not an L-type (levamisole and pyrantel) agonist: Classification of cholinergic anthelmintics in Ascaris","volume":"34","author":"Martin","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1111\/j.1476-5381.2011.01420.x","article-title":"Functional reconstitution of Haemonchus contortus acetylcholine receptors in Xenopus oocytes provides mechanistic insights into levamisole resistance","volume":"164","author":"Boulin","year":"2011","journal-title":"Br. J. Pharmacol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.molbiopara.2009.11.007","article-title":"cDNA-AFLP analysis in levamisole-resistant Haemonchus contortus reveals alternative splicing in a nicotinic acetylcholine receptor subunit","volume":"170","author":"Fauvin","year":"2010","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.molbiopara.2015.12.006","article-title":"Expression of nicotinic acetylcholine receptor subunits from parasitic nematodes in Caenorhabditis elegans","volume":"204","author":"Sloan","year":"2015","journal-title":"Mol. Biochem. Parasitol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/9\/1550\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:44:53Z","timestamp":1760208293000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/22\/9\/1550"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,14]]},"references-count":66,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2017,9]]}},"alternative-id":["molecules22091550"],"URL":"https:\/\/doi.org\/10.3390\/molecules22091550","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,14]]}}}