{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T19:08:47Z","timestamp":1789844927568,"version":"4.0.1"},"reference-count":41,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2017,12,18]],"date-time":"2017-12-18T00:00:00Z","timestamp":1513555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University of Lodz","award":["503\/3-015-01\/503-31-004"],"award-info":[{"award-number":["503\/3-015-01\/503-31-004"]}]},{"name":"Medical University of Lodz","award":["502-03\/3-015-01\/502-34-061"],"award-info":[{"award-number":["502-03\/3-015-01\/502-34-061"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Background: Iminodiacetic acid (IDA) derivatives can be used as ligands to form complexes with technetium, with potential application as hepatobiliary diagnostic agents. The aim of this study was to synthesize five novel IDA derivatives and to compare their effects on plasma haemostasis with clinically approved ligands for technetium complexation. Methods: The influence of synthesized IDA derivatives on plasma haemostasis was evaluated spectrophotometrically by clot formation and lysis test (CL-test), coagulation assay, Prothrombin Time and Activated Partial Tromboplastin Time. The effects of the tested compounds on erythrocytes were assessed using haemolysis assays, microscopy and flow cytometry studies. Results: Despite their significant influence on the kinetic parameters of the process of clot formation and fibrinolysis, the tested ligands, at potential diagnostic concentrations, did not alter the overall potential of clot formation and lysis (CLAUC). At potential diagnostic concentrations (0.4 \u03bcmol\/mL) all the tested compounds showed no adverse effects on the membranes of RBCs (Red Blood Cells). Conclusion: IDA derivatives with methoxy substituents in aromatic ring, exert multidirectional effects on plasma haemostasis and should be considered safe as their significant impacts were mostly observed at 4 \u03bcmol\/mL, which is about 10-fold higher than the theoretical plasma concentrations of these compounds.<\/jats:p>","DOI":"10.3390\/molecules22122265","type":"journal-article","created":{"date-parts":[[2017,12,19]],"date-time":"2017-12-19T03:54:32Z","timestamp":1513655672000},"page":"2265","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Synthesis and Biocompatibility Studies of New Iminodiacetic Acid Derivatives"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1012-6550","authenticated-orcid":false,"given":"Magdalena","family":"Markowicz-Piasecka","sequence":"first","affiliation":[{"name":"Laboratory of Bioanalysis, Department of Pharmaceutical Chemistry, Drug Analysis and Radiopharmacy, Medical University of Lodz, ul. Muszy\u0144skiego1, 90-151 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"D\u0119bski","sequence":"additional","affiliation":[{"name":"Students Research Group, Laboratory of Bioanalysis, Department of Pharmaceutical Chemistry, Drug Analysis and Radiopharmacy, Medical University of Lodz, ul. Muszy\u0144skiego 1, 90-151 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u017cbieta","family":"Mikiciuk-Olasik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Drug Analysis and Radiopharmacy, Medical University of Lodz, ul. Muszy\u0144skiego 1, 90-151 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Sikora","sequence":"additional","affiliation":[{"name":"Laboratory of Bioanalysis, Department of Pharmaceutical Chemistry, Drug Analysis and Radiopharmacy, Medical University of Lodz, ul. Muszy\u0144skiego1, 90-151 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,12,18]]},"reference":[{"key":"ref_1","unstructured":"\u015awieczko-\u017burek, B. (2009). Biomateria\u0142y (Biomaterials), Wydawnictwo Politechniki Gda\u0144skiej. Biomedical Engineering."},{"key":"ref_2","unstructured":"U.S. Department of Health and Human Services, Food and Drug Administration, and Center for Devices and Radiological Health (2016). Use of International Standard ISO 10993-1, Biological Evaluation of Medical Devices\u2014Part 1: Evaluation and Testing within a Risk Management Process, Guidance for Industry and Food and Drug Administration Staff."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Pereira, B.T., Ferreira Silva, E., Gon\u00e7alves, M.A., Mancini, D.T., and Ramalho, T.C. (2017). 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