{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:39:44Z","timestamp":1779104384941,"version":"3.51.4"},"reference-count":32,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T00:00:00Z","timestamp":1513900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Carnitine palmitoyltransferase-2 (CPT2) is a mitochondrial enzyme involved in long-chain fatty acid entry into mitochondria for their \u03b2-oxidation and energy production. Two phenotypes are associated with the extremely reduced CPT2 activity in genetically deficient patients: neonatal lethality or, in milder forms, myopathy. Resveratrol (RSV) is a phytophenol produced by grape plant in response to biotic or abiotic stresses that displays anti-oxidant properties, in particular through AP-1, NF\u03baB, STAT-3, and COX pathways. Some beneficiary effects of RSV are due to its modulation of microRNA (miRNA) expression. RSV can enhance residual CPT2 activities in human fibroblasts derived from CPT2-deficient patients and restores normal fatty acid oxidation rates likely through stimulation of mitochondrial biogenesis. Here, we report changes in miRNA expression linked to CPT2-deficiency, and we identify miRNAs whose expression changed following RSV treatment of control or CPT2-deficient fibroblasts isolated from patients. Our findings suggest that RSV consumption might exert beneficiary effects in patients with CPT2-deficiency.<\/jats:p>","DOI":"10.3390\/molecules23010007","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T05:50:19Z","timestamp":1513921819000},"page":"7","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Resveratrol-Induced Changes in MicroRNA Expression in Primary Human Fibroblasts Harboring Carnitine-Palmitoyl Transferase-2 Gene Mutation, Leading to Fatty Acid Oxidation Deficiency"],"prefix":"10.3390","volume":"23","author":[{"given":"Virginie","family":"Aires","sequence":"first","affiliation":[{"name":"Laboratory BioPeroxIL, University of Bourgogne-Franche Comt\u00e9, 6 Blvd Gabriel, 21000 Dijon, France"},{"name":"INSERM UMR 866, Blvd Jeanne d\u2019Arc, 21490 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Delmas","sequence":"additional","affiliation":[{"name":"Laboratory BioPeroxIL, University of Bourgogne-Franche Comt\u00e9, 6 Blvd Gabriel, 21000 Dijon, France"},{"name":"INSERM UMR 866, Blvd Jeanne d\u2019Arc, 21490 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima","family":"Djouadi","sequence":"additional","affiliation":[{"name":"INSERM U1124, Universit\u00e9 Paris-Descartes, Rue des Saints-P\u00e8res, 75000 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Bastin","sequence":"additional","affiliation":[{"name":"INSERM U1124, Universit\u00e9 Paris-Descartes, Rue des Saints-P\u00e8res, 75000 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustapha","family":"Cherkaoui-Malki","sequence":"additional","affiliation":[{"name":"Laboratory BioPeroxIL, University of Bourgogne-Franche Comt\u00e9, 6 Blvd Gabriel, 21000 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6794-2690","authenticated-orcid":false,"given":"Norbert","family":"Latruffe","sequence":"additional","affiliation":[{"name":"Laboratory BioPeroxIL, University of Bourgogne-Franche Comt\u00e9, 6 Blvd Gabriel, 21000 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6053","DOI":"10.2174\/1381612811319340002","article-title":"Bioactive polyphenols from grape and wine","volume":"19","author":"Latruffe","year":"2013","journal-title":"Curr. Pharm. Des."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/biof.108","article-title":"Biosynthesis, metabolism, molecular engineering, and biological functions of stilbene phytoalexins in plants","volume":"36","author":"Jeandet","year":"2010","journal-title":"BioFactors"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"18033","DOI":"10.3390\/molecules191118033","article-title":"Deciphering the role of phytoalexins in plant-microorganism interactions and human health","volume":"19","author":"Jeandet","year":"2014","journal-title":"Molecules"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1093\/carcin\/bgq143","article-title":"Resveratrol decreases the levels of miR-155 by upregulating miR-663, a microRNA targeting JunB and JunD","volume":"31","author":"Tili","year":"2010","journal-title":"Carcinogenesis"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Limagne, E., Lan\u00e7on, A., Delmas, D., Cherkaoui-Malki, M., and Latruffe, N. (2016). Resveratrol Interferes with IL1-\u03b2-Induced Pro-Inflammatory Paracrine Interaction between Primary Chondrocytes and Macrophages. Nutrients, 8.","DOI":"10.3390\/nu8050280"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1002\/jcb.24539","article-title":"Mechanisms of ceramide-induced COX-2-dependent apoptosis in human ovarian cancer OVCAR-3 cells partially overlapped with resveratrol","volume":"114","author":"Lin","year":"2013","journal-title":"J. Cell. Biochem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2048","DOI":"10.1093\/hmg\/ddr089","article-title":"Exposure to resveratrol triggers pharmacological correction of fatty acid utilization in human fatty acid oxidation-deficient fibroblasts","volume":"20","author":"Bastin","year":"2011","journal-title":"Hum. Mol. Genet."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/j.bcp.2012.02.004","article-title":"MicroRNAs in cancer management and their modulation by dietary agents","volume":"83","author":"Karius","year":"2012","journal-title":"Biochem. Pharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1007\/s11095-010-0105-y","article-title":"Regulation of microRNAs by natural agents: An emerging field in chemoprevention and chemotherapy research","volume":"27","author":"Li","year":"2010","journal-title":"Pharm. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.freeradbiomed.2013.05.046","article-title":"MiRNA as molecular target of polyphenols underlying their biological effects","volume":"64","author":"Milenkovic","year":"2013","journal-title":"Free Radic. Biol. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8783","DOI":"10.1021\/jf301479v","article-title":"Control of MicroRNA expression as a new way for resveratrol to deliver its beneficial effects","volume":"60","author":"Kaminski","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1002\/jsfa.6228","article-title":"Effects of dietary phytophenols on the expression of microRNAs involved in mammalian cell homeostasis","volume":"93","author":"Michaille","year":"2013","journal-title":"J. Sci. Food Agric."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/nyas.12819","article-title":"Exploring new ways of regulation by resveratrol involving miRNAs, with emphasis on inflammation","volume":"1348","author":"Latruffe","year":"2015","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2057","DOI":"10.1016\/j.bcp.2010.07.003","article-title":"Resveratrol modulates the levels of microRNAs targeting genes encoding tumor-suppressors and effectors of TGF\u03b2 signaling pathway in SW480 cells","volume":"80","author":"Tili","year":"2010","journal-title":"Biochem. Pharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Kumar, A., Rimando, A.M., and Levenson, A.S. (2017). Resveratrol and pterostilbene as a microRNA-mediated chemopreventive and therapeutic strategy in prostate cancer. Ann. N. Y. Acad. Sci.","DOI":"10.1111\/nyas.13372"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Wu, F., and Cui, L. (2017). Resveratrol suppresses melanoma by inhibiting NF-\u03baB\/miR-221 and inducing TFG expression. Arch. Dermatol. Res.","DOI":"10.1007\/s00403-017-1784-6"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e2104","DOI":"10.1038\/cddis.2016.6","article-title":"Role of apoptosis-related miRNAs in resveratrol-induced breast cancer cell death","volume":"7","author":"Venkatadri","year":"2016","journal-title":"Cell Death Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.3892\/or.2014.3090","article-title":"Synergistic antitumor activity of resveratrol and miR-200c in human lung cancer","volume":"31","author":"Bai","year":"2014","journal-title":"Oncol. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1680","DOI":"10.1039\/C5FO01090J","article-title":"Involvement of miR-539-5p in the inhibition of de novo lipogenesis induced by resveratrol in white adipose tissue","volume":"7","author":"Gracia","year":"2016","journal-title":"Food Funct."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Gracia, A., Fern\u00e1ndez-Quintela, A., Miranda, J., Eseberri, I., Gonz\u00e1lez, M., and Portillo, M.P. (2017). Are miRNA-103, miRNA-107 and miRNA-122 Involved in the Prevention of Liver Steatosis Induced by Resveratrol?. Nutrients, 9.","DOI":"10.3390\/nu9040360"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"231","DOI":"10.3892\/ijmm.2016.2802","article-title":"Resveratrol upregulates SOCS1 production by lipopolysaccharide-stimulated RAW264.7 macrophages by inhibiting miR-155","volume":"39","author":"Ma","year":"2017","journal-title":"Int. J. Mol. Med."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Tili, E., and Michaille, J.J. (2016). Promiscuous Effects of Some Phenolic Natural Products on Inflammation at Least in Part Arise from Their Ability to Modulate the Expression of Global Regulators, Namely microRNAs. Molecules, 21.","DOI":"10.3390\/molecules21091263"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.biopha.2015.08.025","article-title":"MicroRNA-214 participates in the neuroprotective effect of Resveratrol via inhibiting \u03b1-synuclein expression in MPTP-induced Parkinson\u2019s disease mouse","volume":"74","author":"Wang","year":"2015","journal-title":"Biomed. Pharmacother."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.3892\/mmr.2015.3581","article-title":"Resveratrol prevents osteoporosis in ovariectomized rats by regulating microRNA-338-3p","volume":"12","author":"Guo","year":"2015","journal-title":"Mol. Med. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/1750-1172-9-79","article-title":"Stilbenes and resveratrol metabolites improve mitochondrial fatty acid oxidation defects in human fibroblasts","volume":"9","author":"Aires","year":"2014","journal-title":"Orphanet J. Rare Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.1093\/hmg\/ddt603","article-title":"Beneficial effects of resveratrol on respiratory chain defects in patients\u2019 fibroblasts involve estrogen receptor and estrogen-related receptor alpha signaling","volume":"23","author":"Mathieu","year":"2014","journal-title":"Hum. Mol. Genet."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.freeradbiomed.2016.04.027","article-title":"Resveratrol attenuates oxidative stress in mitochondrial Complex I deficiency: Involvement of SIRT3","volume":"96","author":"Mathieu","year":"2016","journal-title":"Free Radical Biol. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.mito.2013.04.002","article-title":"Dual control of mitochondrial biogenesis by sirtuin 1 and sirtuin 3","volume":"13","author":"Brenmoehl","year":"2013","journal-title":"Mitochondrion"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1017\/S0029665115004218","article-title":"Polyphenols and non-alcoholic fatty liver disease: Impact and mechanisms","volume":"75","author":"Vauzour","year":"2016","journal-title":"Proc. Nutr. Soc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.cmet.2012.04.003","article-title":"SIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function","volume":"15","author":"Price","year":"2012","journal-title":"Cell Metab."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Bastin, J., and Djouadi, F. (2016). Resveratrol and Myopathy. Nutrients, 8.","DOI":"10.3390\/nu8050254"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.apsb.2015.01.002","article-title":"Emerging role of microRNAs in lipid metabolism","volume":"5","author":"Yanga","year":"2015","journal-title":"Acta Pharm. Sin. 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