{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T11:44:51Z","timestamp":1770378291621,"version":"3.49.0"},"reference-count":22,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T00:00:00Z","timestamp":1513814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 81260475"],"award-info":[{"award-number":["No. 81260475"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 81260676"],"award-info":[{"award-number":["No. 81260676"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Natural Science Foundation of Yunnan Province","award":["No. 2017FA050"],"award-info":[{"award-number":["No. 2017FA050"]}]},{"name":"the Natural Science Foundation of Yunnan Province","award":["No. 2016FA012"],"award-info":[{"award-number":["No. 2016FA012"]}]},{"name":"the Natural Science Foundation of Yunnan Province","award":["No. 2014FA007"],"award-info":[{"award-number":["No. 2014FA007"]}]},{"name":"Yunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics","award":["No. [2012]25"],"award-info":[{"award-number":["No. [2012]25"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Blaps rynchopetera Fairmaire has long been used as a folk medicine by the Yi and Bai ethnic groups in China to treat fever, cough, gastritis, boils, and tumors. In the present study, the cytotoxicity of the defensive secretion (TDS) of B. rynchopetera against AGS Caco-2, HepG2 U251 and Bel-7402 was tested, and the results revealed that TDS had potent cytotoxicity against testing cells with IC50 values of 45.8, 17.4, 53.6, 98.4 and 23.4 \u03bcg\/mL, respectively. Gas chromatography-mass spectrometry (GC-MS) analysis was employed to clarify the cytotoxic constituents in TDS of B. rynchopetera and five volatile compounds, including 2-ethyl-2,5-cyclohexadiene-1,4-dione (3, 31.00%), 1-tridecene (5, 28.02%), 2-methyl-2,5-cyclohexadiene-1,4-dione (2, 22.86%), hydroquinone (4, 1.33%), and p-benzoquinone (1, 1.01%), were identified. Chemical constituent investigation on TDS further supported the presence of 5 above compounds. A cytotoxic assay indicated that compounds 1, 2, 3 and 4 exhibited significant cytotoxicity against the testing cell lines, implying that benzoquinones and hydroquinone played important roles in the cytotoxicity of TDS of B. rynchopetera. TDS is a cytotoxic natural material and further studies investigating mechanisms and inhibitory activities on other cell lines is warranted.<\/jats:p>","DOI":"10.3390\/molecules23010010","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T12:16:14Z","timestamp":1513858574000},"page":"10","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Cytotoxicity of the Defensive Secretion from the Medicinal Insect Blaps rynchopetera"],"prefix":"10.3390","volume":"23","author":[{"given":"Huai","family":"Xiao","sequence":"first","affiliation":[{"name":"Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, China"},{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&amp;D, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Wei","family":"Dong","sequence":"additional","affiliation":[{"name":"Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di-Jiao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiu-Mei","family":"Wu","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&amp;D, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Rong","family":"Luo","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&amp;D, Dali University, Dali 671000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng-Gui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&amp;D, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na-Na","family":"Guo","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&amp;D, Dali University, Dali 671000, China"},{"name":"Basic Medical College, Chengde Medical University, Chengde 067000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Li","sequence":"additional","affiliation":[{"name":"Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&amp;D, Dali University, Dali 671000, China"},{"name":"National-Local Joint Engineering Research Center of Entomoceutics, Dali 671000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le","family":"Cai","sequence":"additional","affiliation":[{"name":"Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong-Tao","family":"Ding","sequence":"additional","affiliation":[{"name":"Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,12,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1016\/j.fitote.2012.03.002","article-title":"The biosynthetic products of Chinese insect medicine Aspongopus chinensis","volume":"83","author":"Luo","year":"2012","journal-title":"Fitoterapia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.ibmb.2011.05.007","article-title":"Insect natural products and processes: New treatments for human disease","volume":"41","author":"Ratcliffe","year":"2011","journal-title":"Insect Biochem. 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