{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T22:29:24Z","timestamp":1770071364340,"version":"3.49.0"},"reference-count":17,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T00:00:00Z","timestamp":1519603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The aim of the study was to develop an alternative capillary zone electrophoresis (CZE) for simultaneous determination of phillyrin (1), phillygenin (2), epipinoresinol-4-O-\u03b2-glucoside (3), pinoresinol-4-O-\u03b2-glucoside (4), lariciresinol (5), pinoresinol (6), isolariciresinol (7) and vladinol D (8) in Forsythia suspensa. The structural types of lignans 1\u20138 could be attributed to bisepoxylignans (1\u20134 and 6), monoepoxylignans (5 and 8) and cyclolignan (7). The major difficulties in the CZE separation of 1\u20138 could be summarization as the simultaneous presence of free lignans (1, 2 and 5\u20138) and lignan glucosides (3 and 4) and simultaneous occurrence of two pairs of isomers (3 and 4 as well as 5 and 7). Without the addition of \u03b2-CD and methanol, the resolution of these analytes was quite poor. However, in this study, compounds 1\u20138 were excellently separated from each other within 15 min under optimized conditions with a borax running buffer (40 mM borax, pH 10.30) containing 2 mM \u03b2-CD and 5% methanol (v\/v) at the voltage of 20 kV, temperature of 35 \u00b0C and detection wavelength of 234 nm. Validation of the method included tests of linearity, precision, repeatability, stability and accuracy. In addition, the method offered inherent advantages such as lower analytical cost, no need of specific columns and use of small amounts of organic solvents and reagents. Finally, this green and economic CZE was successfully applied for the determination of these bioactive components 1\u20138 in F. suspensa fruits and its commercial extracts.<\/jats:p>","DOI":"10.3390\/molecules23030514","type":"journal-article","created":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T03:36:12Z","timestamp":1519702572000},"page":"514","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Simultaneous Separation of Eight Lignans in Forsythia suspensa by \u03b2-Cyclodextrin-Modified Capillary Zone Electrophoresis"],"prefix":"10.3390","volume":"23","author":[{"given":"Jun","family":"Liang","sequence":"first","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng-Qiu","family":"Gong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-Min","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1016\/j.bmcl.2008.01.115","article-title":"Lignans from the fruits of Forsythia suspensa","volume":"18","author":"Piao","year":"2008","journal-title":"Bioorg. 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