{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T14:02:20Z","timestamp":1783000940189,"version":"3.54.5"},"reference-count":67,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,4,21]],"date-time":"2018-04-21T00:00:00Z","timestamp":1524268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004386","name":"University of Malaya","doi-asserted-by":"publisher","award":["GA001-2016 and GA002-2016"],"award-info":[{"award-number":["GA001-2016 and GA002-2016"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004386","name":"University of Malaya","doi-asserted-by":"publisher","award":["PG136-2016A"],"award-info":[{"award-number":["PG136-2016A"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>The quorum sensing (QS) system has been used by many opportunistic pathogenic bacteria to coordinate their virulence determinants in relation to cell-population density. As antibiotic-resistant bacteria are on the rise, interference with QS has been regarded as a novel way to control bacterial infections. As such, many plant-based natural products have been widely explored for their therapeutic roles. These natural products may contain anti-QS compounds that could block QS signals generation or transmission to combat QS pathogens. In this study, we report the anti-QS activities of four different Chinese herbal plant extracts: Poria cum Radix pini, Angelica dahurica, Rhizoma cibotii and Schizonepeta tenuifolia, on Pseudomonas aeruginosa PAO1. All the plants extracted using hexane, chloroform and methanol were tested and found to impair swarming motility and pyocyanin production in P. aeruginosa PAO1, particularly by Poria cum Radix pini. In addition, all the plant extracts also inhibited violacein production in C. violaceum CV026 up to 50% while bioluminescence activities were reduced in lux-based E. coli biosensors, pSB401 and pSB1075, up to about 57%. These anti-QS properties of the four medicinal plants are the first documentation that demonstrates a potential approach to attenuate pathogens\u2019 virulence determinants.<\/jats:p>","DOI":"10.3390\/molecules23040972","type":"journal-article","created":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T04:44:48Z","timestamp":1524545088000},"page":"972","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["The Effects of Chinese Herbal Medicines on the Quorum Sensing-Regulated Virulence in Pseudomonas aeruginosa PAO1"],"prefix":"10.3390","volume":"23","author":[{"given":"Yee Meng","family":"Chong","sequence":"first","affiliation":[{"name":"Division of Genetics and Molecular Biology, ISB, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9193-5503","authenticated-orcid":false,"given":"Kah Yan","family":"How","sequence":"additional","affiliation":[{"name":"Division of Genetics and Molecular Biology, ISB, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wai Fong","family":"Yin","sequence":"additional","affiliation":[{"name":"Division of Genetics and Molecular Biology, ISB, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1883-1115","authenticated-orcid":false,"given":"Kok Gan","family":"Chan","sequence":"additional","affiliation":[{"name":"Vice Chancellor Office, Jiangsu University, Zhenjiang 212013, China"},{"name":"Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,21]]},"reference":[{"key":"ref_1","unstructured":"Sleigh, J.D., and Timbury, M.C. (1998). Notes on Medical Bacteriology, Churchill Livingstone. [5th ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1128\/AEM.68.4.1754-1759.2002","article-title":"Identification of quorum-quenching N-acyl homoserine lactonases from Bacillus species","volume":"68","author":"Dong","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6902","DOI":"10.1128\/JB.186.20.6902-6914.2004","article-title":"Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyi","volume":"186","author":"Henke","year":"2004","journal-title":"J. Bacteriol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1073\/pnas.92.5.1490","article-title":"A second N-acylhomoserine lactone signal produced by Pseudomonas aeruginosa","volume":"92","author":"Pearson","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/j.jasms.2004.02.012","article-title":"Electrospray\/Mass spectrometric identification and analysis of 4-hydroxy-2-alkylquinolones (HAQs) produced by Pseudomonas aeruginosa","volume":"15","author":"Lepine","year":"2004","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/nchembio.1225","article-title":"A cell-cell communication signal integrates quorum sensing and stress response","volume":"9","author":"Lee","year":"2013","journal-title":"Nat. Chem. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1007\/s13238-014-0100-x","article-title":"The hierarchy quorum sensing network in Pseudomonas aeruginosa","volume":"6","author":"Lee","year":"2015","journal-title":"Protein Cell"},{"key":"ref_8","unstructured":"Mandell, G.L., Dolan, R., and Bennett, J.E. (1995). Pseudomonas aeruginosa. Principles and Practices of Infectious Diseases, Churchill Livingstone."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.vetmic.2008.02.018","article-title":"Antimicrobial resistance and genetic characterization of fluoroquinolone resistance of Pseudomonas aeruginosa isolated from canine infections","volume":"131","author":"Rubin","year":"2008","journal-title":"Vet. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1099\/mic.0.28601-0","article-title":"Quorum sensing inhibitors: A bargain of effects","volume":"152","author":"Rasmussen","year":"2006","journal-title":"Microbiology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6618","DOI":"10.1128\/jb.178.22.6618-6622.1996","article-title":"Eukaryotic interference with homoserine lactone-mediated prokaryotic signaling","volume":"178","author":"Givskov","year":"1996","journal-title":"J. Bacteriol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6217","DOI":"10.3390\/s130506217","article-title":"Plant-derived natural products as sources of anti-quorum sensing compounds","volume":"13","author":"Koh","year":"2013","journal-title":"Sensors"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.tim.2017.10.005","article-title":"Quorum-Sensing Systems as Targets for Antivirulence Therapy","volume":"26","author":"Defoirdt","year":"2018","journal-title":"Trends Microbiol."},{"key":"ref_14","unstructured":"Chin, W.Y., and Keng, H. (1992). An Illustrated Dictionary of Chinese Medical Herbs, CRCS Publications."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1136\/bmj.319.7216.1050","article-title":"ABC of complementary medicine: Herbal medicine","volume":"319","author":"Vickers","year":"1999","journal-title":"Br. Med. J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1172\/JCI20074","article-title":"Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections","volume":"112","author":"Hentzer","year":"2003","journal-title":"J. Clin. Investig."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.tifs.2015.02.009","article-title":"Plant food extracts and phytochemicals: Their role as Quorum Sensing Inhibitors","volume":"43","author":"Truchado","year":"2015","journal-title":"Trends Food Sci. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.1021\/np100872k","article-title":"Malabaricone C from Myristica cinnamomea exhibits anti-quorum sensing activity","volume":"74","author":"Chong","year":"2011","journal-title":"J. Nat. Prod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4339","DOI":"10.3390\/s120404339","article-title":"Silencing quorum sensing through extracts of Melicop lunu-ankenda","volume":"12","author":"Tan","year":"2012","journal-title":"Sensors"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"14558","DOI":"10.3390\/s131114558","article-title":"Anti-quorum sensing activity of the Chinese traditional herb. Phyllantus amarus","volume":"13","author":"Priya","year":"2013","journal-title":"Sensors"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1128\/JB.187.5.1799-1814.2005","article-title":"Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI Selector","volume":"187","author":"Rasmussen","year":"2005","journal-title":"J. Bacteriol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1094\/MPMI.2000.13.6.637","article-title":"Plants secrete substances that mimic bacterial N-acyl homoserine lactone signal activities and affect population density-dependent behaviors in associated bacteria","volume":"13","author":"Teplitski","year":"2000","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.jep.2005.11.025","article-title":"Anti-quorum sensing activity of medicinal plants in southern Florida","volume":"105","author":"Adonizio","year":"2006","journal-title":"J. Enthnopharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4284","DOI":"10.1128\/aem.62.11.4284-4287.1996","article-title":"Inhibitory effects of secondary metabolites from the red alga Delisea pulchra on swarming motility of Proteus Mirabilis","volume":"62","author":"Gram","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3803","DOI":"10.1093\/emboj\/cdg366","article-title":"Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors","volume":"22","author":"Hentzer","year":"2003","journal-title":"EMBO J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3703","DOI":"10.1099\/00221287-143-12-3703","article-title":"Quorum sensing and Chromobacterium violaceum: Exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones","volume":"143","author":"McClean","year":"1997","journal-title":"Microbiology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8307","DOI":"10.1016\/j.bmc.2006.09.013","article-title":"Antioxidant properties of violacein: Possible relation on its biological function","volume":"14","author":"Konzen","year":"2006","journal-title":"Bioorg. Med. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/j.1574-6968.1998.tb13044.x","article-title":"Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing","volume":"163","author":"Winson","year":"1998","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/S1473-3099(02)00447-4","article-title":"Controlling infection by tuning in and turning down the volume of bacterial small-talk","volume":"2","author":"Williams","year":"2002","journal-title":"Lancet Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7155","DOI":"10.1128\/jb.177.24.7155-7163.1995","article-title":"Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family","volume":"177","author":"Brint","year":"1995","journal-title":"J. Bacteriol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6472","DOI":"10.1128\/JB.184.23.6472-6480.2002","article-title":"Functions required for extracellular quinolone signaling by Pseudomonas aeruginosa","volume":"184","author":"Gallagher","year":"2002","journal-title":"J. Bacteriol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.molmed.2004.10.002","article-title":"The role of pyocyanin in Pseudomonas aeruginosa infection","volume":"10","author":"Lau","year":"2004","journal-title":"Trends Mol. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1111\/j.1574-6976.2005.00012.x","article-title":"Regulation of quorum sensing in Pseudomonas","volume":"30","author":"Venturi","year":"2006","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5990","DOI":"10.1128\/JB.182.21.5990-5996.2000","article-title":"Swarming of Pseudomonas aeruginosa is dependent on cell-to-cell signaling and requires flagella and pili","volume":"182","author":"Curty","year":"2000","journal-title":"J. Bacteriol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1111\/j.1365-2958.1994.tb00433.x","article-title":"Bees aren\u2019t the only ones: Swarming in Gram-negative bacteria","volume":"13","author":"Harshey","year":"1994","journal-title":"Mol. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4885","DOI":"10.1073\/pnas.060030097","article-title":"Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities in Pseudomonas aeruginosa","volume":"97","author":"Rashid","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1099\/00221287-148-4-1119","article-title":"Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover","volume":"148","author":"Manefield","year":"2002","journal-title":"Microbiology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"M2538","DOI":"10.1111\/1750-3841.13431","article-title":"Quorum Quenching and Microbial Control through Phenolic Extract of Eugenia Uniflora Fruits","volume":"81","author":"Rodrigues","year":"2016","journal-title":"J. Food Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1590\/1678-457X.0089","article-title":"Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)","volume":"36","author":"Rodrigues","year":"2016","journal-title":"Food Sci. Technol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.indcrop.2016.01.037","article-title":"Antioxidant, antimicrobial and anti-quorum sensing activities of Rubus rosaefolius phenolic extract","volume":"84","author":"Oliveira","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.jmii.2014.03.012","article-title":"Anti-quorum sensing activity of flavonoid-rich fraction from Centella asiatica L. against Pseudomonas aeruginosa PAO1","volume":"49","author":"Vasavi","year":"2016","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1128\/AEM.01059-09","article-title":"Identification of catechin as one of the flavonoids from Combretum albiflorum bark extract that reduces the production of quorum-sensing-controlled virulence factors in Pseudomonas aeruginosa PAO1","volume":"76","author":"Vandeputte","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1099\/mic.0.064378-0","article-title":"Endemic malagasy Dalbergia species inhibit quorum sensing in Pseudomonas aeruginosa PAO1","volume":"159","author":"Rasamiravaka","year":"2013","journal-title":"Microbiology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.apjtm.2017.07.021","article-title":"Medicinal plant products targeting quorum sensing for combating bacterial infections","volume":"10","author":"Bouyahya","year":"2017","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4064","DOI":"10.1074\/jbc.M116.770552","article-title":"Flavonoids Suppress Pseudomonas aeruginosa Virulence through Allosteric Inhibition of Quorum-sensing Receptors","volume":"292","author":"Paczkowski","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.jep.2012.07.004","article-title":"Pomegranate peel and fruit extracts: A review of potential anti-inflammatory and anti-infective effects","volume":"143","author":"Ismail","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.foodcont.2011.09.006","article-title":"Food phytochemicals act as quorum sensing inhibitors reducing production and\/or degrading autoinducers of Yersinia enterocolitica and Erwinia carotovora","volume":"24","author":"Truchado","year":"2012","journal-title":"Food Control"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Starkey, M., Lepine, F., Maura, D., Bandyopadhaya, A., Lesic, B., He, J., Kitao, T., Righi, V., Milot, S., and Tzika, A. (2014). Identification of anti-virulence compounds that disrupt quorum-sensing regulated acute and persistent pathogenicity. PLoS Pathog., 10.","DOI":"10.1371\/journal.ppat.1004321"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3589","DOI":"10.1099\/mic.0.27954-0","article-title":"The LuxR receptor: The sites of interaction with quorum-sensing signals and inhibitors","volume":"151","author":"Koch","year":"2005","journal-title":"Microbiology"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"6840","DOI":"10.1128\/AEM.02378-13","article-title":"Resistance to quorum-quenching compounds","volume":"79","author":"Maeda","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.mib.2016.06.001","article-title":"Considerations and caveats in anti-virulence drug development","volume":"33","author":"Maura","year":"2016","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Chan, K.G., Atkinson, S., Mathee, K., Sam, C.K., Chhabra, S.R., C\u00e1mara, M., Koh, C.L., and Williams, P. (2011). Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia. BMC Microbiol., 11.","DOI":"10.1186\/1471-2180-11-51"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0254-6272(10)60004-0","article-title":"Clinical Observation on the Combined Use of Acupuncture and Herbal Medicine for Treatment of Chloasma","volume":"30","author":"Feng","year":"2010","journal-title":"J. Tradit. Chin. Med."},{"key":"ref_54","first-page":"47","article-title":"A new pregnane steroid from Poria cum radix pini","volume":"95","author":"Tong","year":"2010","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Kimura, T., But, P.P.H., Guo, J.X., and Sung, C.K. (1996). International Collection of Traditional and Folk Medicine: Part 1, World Scientific.","DOI":"10.1142\/3041"},{"key":"ref_56","unstructured":"Yang, S.Z. (1998). The Divine Farmer\u2019s Materia Medica, Blue Poppy Press."},{"key":"ref_57","unstructured":"Pei, G.X., Da, P.L., and Shi, L.Y. (2002). New Edited Record of TCM (Volume 1), China Chemical Industry Press."},{"key":"ref_58","first-page":"160","article-title":"The progress in research of Cibotium barometz","volume":"23","author":"Xu","year":"2000","journal-title":"J. Chin. Med. Mater."},{"key":"ref_59","unstructured":"Zhao, Y., Zhu, J.Y., and Zhang, G.J. (2007). Chinese Medicine (Basis and Application of TCM), People\u2019s Medical Publishing House."},{"key":"ref_60","unstructured":"China Pharmacopoeia Committee (2010). Chinese Pharmacopoeia (Volume 1), Chemical Industry Press."},{"key":"ref_61","unstructured":"Chang, H.M., and But, P.P.H. (1986). Pharmacology and Applications of Chinese Materia Medica (Volume 2), World Scientific."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1007\/BF02986008","article-title":"Anti-diabetic agents from medicinal plants inhibitory activity of Schizonepeta tenuifolia spikes on the diabetogenesis by Streptozotocin in mice","volume":"19","author":"Kim","year":"1996","journal-title":"Arch. Pharm. Res."},{"key":"ref_63","unstructured":"Zhu, Y.P. (1998). Chinese Materia Medica: Chemistry, Pharmacology and Applications, CRC Press."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.foodchem.2008.04.044","article-title":"Mechanism of action of Melaleuca armillaris [Sol. Ex Gaertu] Sm. essential oil on six LAB strains as assessed by multiparametric flow cytometry and automated microtiter-based assay","volume":"111","author":"Hayouni","year":"2008","journal-title":"Food Chem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2576","DOI":"10.1128\/JB.184.10.2576-2586.2002","article-title":"Advancing the Quorum Sensing in Pseudomonas aeruginosa: MvaT and the Regulation of N-Acylhomoserine Lactone Production and Virulence Gene Expression","volume":"184","author":"Diggle","year":"2002","journal-title":"J. Bacteriol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"8750","DOI":"10.1128\/JB.01109-07","article-title":"The Wetting Agent Required for Swarming in Salmonella enteric Serovar Typhimurium is Not a Surfactant","volume":"189","author":"Chen","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"3127","DOI":"10.1128\/jb.179.10.3127-3132.1997","article-title":"Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa","volume":"179","author":"Pesci","year":"1997","journal-title":"J. Bacteriol."}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/23\/4\/972\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:01:36Z","timestamp":1760194896000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/23\/4\/972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,21]]},"references-count":67,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,4]]}},"alternative-id":["molecules23040972"],"URL":"https:\/\/doi.org\/10.3390\/molecules23040972","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,21]]}}}