{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:54:19Z","timestamp":1789430059676,"version":"build-2803163510"},"reference-count":42,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2018,5,9]],"date-time":"2018-05-09T00:00:00Z","timestamp":1525824000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Natural and bioactive products have been of great interest due to their benefit as health foods and drugs to prevent various diseases. The aim of this study is to efficiently reuse marine chitinous materials (CMs), abundant and low-cost fishery by-products, for the bio-synthesis, isolation, and identification of antioxidant compounds possessing some other beneficial bioactivities. Paenibacillus sp. was used to convert CMs to antioxidants. Among various tested CMs, squid pen powder (SPP) gave the best results when used as the sole carbon\/nitrogen source. Fermented SPP (FSPP) had comparable antioxidant activity (IC50 = 124 \u00b5g\/mL) to that of \u03b1-tocopherol (IC50 = 30 \u00b5g\/mL). The antioxidant productivity increased 1.83-fold after culture optimization. The use of multiple techniques, including Diaion, silica, and preparative HPLC columns coupled with a bioassay resulted in the isolation of two major antioxidants characterized as exopolysaccharides and homogentisic acid. These isolated compounds showed great maximum activity and low IC50 values (96%, 30 \u00b5g\/mL and 99%, 5.4 \u00b5g\/mL, respectively) which were comparable to that of \u03b1-tocopherol (95%, 24 \u00b5g\/mL). The crude sample, fractions, and isolated compounds also demonstrated \u03b1-glucosidase inhibition and anti\u2013inflammatory activity. Notably, homogentisic acid was found as a non-sugar-based moiety \u03b1-glucosidase inhibitor which show much higher inhibition (IC50 = 215 \u00b5g\/mL) than that of acarbose (IC50 = 1324 \u00b5g\/mL) and also possessed acceptable anti\u2013inflammatory activity (IC50 = 9.8 \u00b5g\/mL). The results highlighted the value of using seafood processing by-products, like squid pens, for the production of several compounds possessing multi-benefit bioactivities and no cytotoxicity.<\/jats:p>","DOI":"10.3390\/molecules23051124","type":"journal-article","created":{"date-parts":[[2018,5,10]],"date-time":"2018-05-10T03:48:27Z","timestamp":1525924107000},"page":"1124","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Production and Bioactivity-Guided Isolation of Antioxidants with \u03b1-Glucosidase Inhibitory and Anti-NO Properties from Marine Chitinous Materials"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2666-9584","authenticated-orcid":false,"given":"Van Bon","family":"Nguyen","sequence":"first","affiliation":[{"name":"Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Hanh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9379-0923","authenticated-orcid":false,"given":"Chien Thang","family":"Doan","sequence":"additional","affiliation":[{"name":"Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam"},{"name":"Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Ngoc","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam"},{"name":"Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2335-1084","authenticated-orcid":false,"given":"Anh Dzung","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot City 630000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao-Haur","family":"Kuo","sequence":"additional","affiliation":[{"name":"Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei 11221, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1384-3731","authenticated-orcid":false,"given":"San-Lang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan"},{"name":"Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.ijbiomac.2012.11.003","article-title":"Evaluation of in vitro antioxidant potency of exopolysaccharide from endophytic Fusarium solani SD5","volume":"53","author":"Mahapatra","year":"2013","journal-title":"Int. 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