{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:08:23Z","timestamp":1790251703079,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T00:00:00Z","timestamp":1532476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Algal lipids have gained wide interest in various applications ranging from biofuels to nutraceuticals. Given their complex nature composed of different lipid classes, a deep knowledge between extraction conditions and lipid characteristics is essential. In this paper, we investigated the influence of different pretreatments on lipid extraction with supercritical CO2 by a lipidomic approach. Pretreatment was found to double the total extraction yield, thereby reaching 23.1 wt.% comparable to the 26.9 wt.% obtained with chloroform\/methanol. An increase in acylglycerides was concurrently observed, together with a nearly doubling of free fatty acids indicative of partial hydrolysis. Moreover, an alteration in the distribution of glyco- and phospholipids was noted, especially promoting digalactosyldiglycerides and phosphatidylcholine as compared to monogalactosyldiglycerides and phosphatidylglycerol. At optimized conditions, supercritical CO2 extraction provided a lipid extract richer in neutral lipids and poorer in phospholipids as compared to chloroform\/methanol, though with a very similar fatty acid distribution within each lipid class.<\/jats:p>","DOI":"10.3390\/molecules23081854","type":"journal-article","created":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T11:09:30Z","timestamp":1532516970000},"page":"1854","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Supercritical CO2 Extraction of Nannochloropsis sp.: A Lipidomic Study on the Influence of Pretreatment on Yield and Composition"],"prefix":"10.3390","volume":"23","author":[{"given":"Kathy","family":"Elst","sequence":"first","affiliation":[{"name":"Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miranda","family":"Maesen","sequence":"additional","affiliation":[{"name":"Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Griet","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leen","family":"Bastiaens","sequence":"additional","affiliation":[{"name":"Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9005-1930","authenticated-orcid":false,"given":"Stefan","family":"Voorspoels","sequence":"additional","affiliation":[{"name":"Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Servaes","sequence":"additional","affiliation":[{"name":"Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/molecules21050551","article-title":"Polysaccharides from the marine environment with pharmacological, cosmeceutical and nutraceutical potential","volume":"21","author":"Ruocco","year":"2016","journal-title":"Molecules"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.livsci.2017.09.020","article-title":"Microalgae as feed ingredients for livestock production and meat quality: A review","volume":"205","author":"Madeira","year":"2017","journal-title":"Livest. 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