{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:04:23Z","timestamp":1777496663683,"version":"3.51.4"},"reference-count":68,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,9,20]],"date-time":"2018-09-20T00:00:00Z","timestamp":1537401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","award":["PROCAD-NF-2008"],"award-info":[{"award-number":["PROCAD-NF-2008"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>This study evaluated the influence of the extract of Eugenia uniflora in adhesion to human buccal epithelial cells (HBEC) biofilm formation and cell surface hydrophobicity (CSH) of Candida spp. isolated from the oral cavity of kidney transplant patients. To evaluate virulence attributes in vitro, nine yeasts were grown in the presence and absence of 1000 \u03bcg\/mL of the extract. Adhesion was quantified using the number of Candida cells adhered to 150 HBEC determined by optical microscope. Biofilm formation was evaluated using two methodologies: XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) and crystal violet assay, and further analyzed by electronic scan microscopy. CSH was quantified with the microbial adhesion to hydrocarbons test. We could detect that the extract of E. uniflora was able to reduce adhesion to HBEC and CSH for both Candida albicans and non-Candida albicans Candida species. We also observed a statistically significant reduced ability to form biofilms in biofilm-producing strains using both methods of quantification. However, two highly biofilm-producing strains of Candida tropicalis had a very large reduction in biofilm formation. This study reinforces the idea that besides growth inhibition, E. uniflora may interfere with the expression of some virulence factors of Candida spp. and may be possibly applied in the future as a novel antifungal agent.<\/jats:p>","DOI":"10.3390\/molecules23102418","type":"journal-article","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T03:46:35Z","timestamp":1537501595000},"page":"2418","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Influence of Eugenia uniflora Extract on Adhesion to Human Buccal Epithelial Cells, Biofilm Formation, and Cell Surface Hydrophobicity of Candida spp. from the Oral Cavity of Kidney Transplant Recipients"],"prefix":"10.3390","volume":"23","author":[{"given":"Luanda","family":"Souza","sequence":"first","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas, Universidade Federal do Rio Grande do Norte-UFRN, Rua Gal, Gustavo Cordeiro de Faria, S\/N, Petr\u00f3polis, Natal-RN 59084-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walicyranison","family":"Silva-Rocha","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas, Universidade Federal do Rio Grande do Norte-UFRN, Rua Gal, Gustavo Cordeiro de Faria, S\/N, Petr\u00f3polis, Natal-RN 59084-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8668-6223","authenticated-orcid":false,"given":"Magda","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Pernambuco-UFPE, Recife-PE 50670-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz","family":"Soares","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Farmac\u00eauticas, Universidade Federal de Pernambuco-UFPE, Recife-PE 50670-901, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2525-9046","authenticated-orcid":false,"given":"Terezinha","family":"Svidzinski","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Universidade Estadual de Maring\u00e1-UEM, Avenida Colombo, 5790, Maring\u00e1-PR 87020-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eveline","family":"Milan","sequence":"additional","affiliation":[{"name":"Departamento de Infectologia, Universidade Federal do Rio Grande do Norte-UFRN, Rua Conego Monte, 110, Quintas, Natal-RN 59084-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina","family":"Pires","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia Cl\u00ednica, Departamento de An\u00e1lises Cl\u00ednicas, Universidade Estadual Paulista-UNESP, Araraquara-SP 14801-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Fusco Almeida","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia Cl\u00ednica, Departamento de An\u00e1lises Cl\u00ednicas, Universidade Estadual Paulista-UNESP, Araraquara-SP 14801-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Mendes-Giannini","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Micologia Cl\u00ednica, Departamento de An\u00e1lises Cl\u00ednicas, Universidade Estadual Paulista-UNESP, Araraquara-SP 14801-902, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilherme","family":"Maranh\u00e3o Chaves","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas, Universidade Federal do Rio Grande do Norte-UFRN, Rua Gal, Gustavo Cordeiro de Faria, S\/N, Petr\u00f3polis, Natal-RN 59084-100, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"22434","DOI":"10.3402\/jom.v5i0.22434","article-title":"Interactions of Candida albicans with host epithelial surfaces","volume":"5","author":"Williams","year":"2013","journal-title":"J. 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