{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T15:08:54Z","timestamp":1766848134837,"version":"build-2065373602"},"reference-count":31,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,10,16]],"date-time":"2018-10-16T00:00:00Z","timestamp":1539648000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>New derivatives of pyrazolo[3,4-b]pyrazines and related heterocycles were synthesized using 5-amino-3-methyl-4-nitroso-1-phenyl-pyrazole (1) as a starting material. The 5-acetyl derivative 15 was shown to be a useful key intermediate for the synthesis of several derivatives of pyrazolopyrazines. Some of the prepared compounds were evaluated for their anti-inflammatory and anti-breast cancer MCF-7 cell line activities. SAR study showed that compounds 15 and 29 exhibited remarkable anti-inflammatory activity, where 15 showed the same activity as that of the reference drug indomethacin. On the other hand, compounds 25i, 25j showed very significant inhibitory activity (p &lt; 0.001) against MCF-7 breast cancer cell line.<\/jats:p>","DOI":"10.3390\/molecules23102657","type":"journal-article","created":{"date-parts":[[2018,10,16]],"date-time":"2018-10-16T11:07:51Z","timestamp":1539688071000},"page":"2657","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Anticancer and Anti-Inflammatory Activities of Some New Pyrazolo[3,4-b]pyrazines"],"prefix":"10.3390","volume":"23","author":[{"given":"Hussein","family":"El-Kashef","sequence":"first","affiliation":[{"name":"Chemistry Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Talaat","family":"El-Emary","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Verhaeghe","sequence":"additional","affiliation":[{"name":"LCC-CNRS Universit\u00e9 de Toulouse, CNRS, UPS, 205 Route de Narbonne, 3107 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Vanelle","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, CNRS, Institut de Chimie Radicalaire ICR, UMR 7273, Laboratoire de Pharmaco-Chimie Radicalaire, Facult\u00e9 de Pharmacie, 27 Boulevard Jean Moulin CS 30064, 13385 Marseille CEDEX 05, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maha","family":"Samy","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,16]]},"reference":[{"key":"ref_1","first-page":"33489a","article-title":"Pyrazolopyrazines and Their Use as Adenosine Antagonists","volume":"135","author":"Akahane","year":"2001","journal-title":"Chem. 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