{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:49:22Z","timestamp":1787161762168,"version":"build-2736575974"},"reference-count":41,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T00:00:00Z","timestamp":1540771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002488","name":"Hannam University","doi-asserted-by":"publisher","award":["2018A122"],"award-info":[{"award-number":["2018A122"]}],"id":[{"id":"10.13039\/501100002488","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Osteoarthritis (OA) is the common form of arthritis and is characterized by disability and cartilage degradation. Although natural product extracts have been reported to have anti-osteoarthritic effects, the potential bioactivity of Ryupunghwan (RPH), a traditional Korean medicinal botanical formula that contains Astragalus membranaceus, Turnera diffusa, Achyranthes bidentata, Angelica gigas, Eclipta prostrata, Eucommia ulmoides, and Ilex paraguariensis, is not known well. Therefore, the inhibitory effects of single compounds isolated from RPH on the OA-related molecules were investigated using IL-1\u03b2-stimulated chondrosarcoma SW1353 (SW1353) cell model. Two bioactive compounds, isomucronulatol 7-O-\u03b2-d-glucoside (IMG) and ecliptasaponin A (ES) were isolated and purified from RPH using column chromatography, and then the structures were analyzed using ESI-MS, 1H-NMR, and 13C-NMR spectrum. The expression or amount of matrix metalloproteinase 13 (MMP13), COX1\/2, TNF-\u03b1, IL-1\u03b2 or p65 was determined by RT-PCR, Western blot, and enzyme-linked immunosorbent assay (ELISA). RPH pretreatment reduced the expression and amounts of MMP13, and the expression of collagen II, COX1\/2, TNF-\u03b1, IL-1\u03b2 or p65, which were increased in IL-1\u03b2-stimulated SW1353 cells. IMG reduced the expression of all OA-related molecules, but the observed inhibitory effect was less than that of RPH extract. The other single compound ES showed the reduced expression of all OA-related molecules, and the effect was stronger than that in IMG (approximately 100 fold). Combination pretreatment of both single components remarkably reduced the expression of MMP13, compared to each single component. These synergic effects may provide potential molecular modes of action for the anti-osteoarthritic effects of RPH observed in clinical and animal studies.<\/jats:p>","DOI":"10.3390\/molecules23112807","type":"journal-article","created":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T11:10:41Z","timestamp":1540811441000},"page":"2807","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Inhibition of Osteoarthritis-Related Molecules by Isomucronulatol 7-O-\u03b2-d-glucoside and Ecliptasaponin A in IL-1\u03b2-Stimulated Chondrosarcoma Cell Model"],"prefix":"10.3390","volume":"23","author":[{"given":"Gwan Ui","family":"Hong","sequence":"first","affiliation":[{"name":"Life &amp; Science Research Center, Hyunsung Vital Co. Ltd., Seoul 07255, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung-Yun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Hannam University, Daejeon 34054, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Hannam University, Daejeon 34054, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Yang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Hannam University, Daejeon 34054, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Yeo","family":"Park","sequence":"additional","affiliation":[{"name":"Life &amp; Science Research Center, Hyunsung Vital Co. Ltd., Seoul 07255, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Young","family":"Hong","sequence":"additional","affiliation":[{"name":"Life &amp; Science Research Center, Hyunsung Vital Co. Ltd., Seoul 07255, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youn Ho","family":"Shin","sequence":"additional","affiliation":[{"name":"Life &amp; Science Research Center, Hyunsung Vital Co. Ltd., Seoul 07255, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Hoon","family":"Jung","sequence":"additional","affiliation":[{"name":"Life &amp; Science Research Center, Hyunsung Vital Co. Ltd., Seoul 07255, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung-Bae","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Bio Quality Control, Korea Bio Polytechnic, Chungnam 32943, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Choongnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8069-8212","authenticated-orcid":false,"given":"Young-In","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Hannam University, Daejeon 34054, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jai Youl","family":"Ro","sequence":"additional","affiliation":[{"name":"Life &amp; Science Research Center, Hyunsung Vital Co. Ltd., Seoul 07255, Korea"},{"name":"Department of Pharmacy, Sungkyunkwan University, Suwon 03063, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.3109\/13880209.2013.879188","article-title":"Pharmaceutical properties of calycosin, the major bioactive isoflavonoid in the dry root extract of Radix astragali","volume":"52","author":"Gao","year":"2014","journal-title":"Pharm. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.jep.2012.02.030","article-title":"Gastroprotective activities of Turnera diffusa Willd. ex Schult. revisited: Role of arbutin","volume":"141","author":"Taha","year":"2012","journal-title":"J. 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