{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:37:11Z","timestamp":1790620631866,"version":"4.1.0"},"reference-count":53,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T00:00:00Z","timestamp":1543536000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PAPIIT-DGAPA, UNAM","award":["IN217415"],"award-info":[{"award-number":["IN217415"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Antigenic fractions of 100, 50, 37, and 28 kDa obtained through the SDS-PAGE method that were more frequently recognized by anti-Coccidioides antibodies in the sera of coccidioidomycosis patients were selected using western blotting. Subsequently, these bands were sequenced, and the obtained proteins were analysed by BLAST to choose peptides specific for Coccidioides spp. from among the shared aligned sequences of related fungi. A peptide specific for C. immitis was selected from the \u201cGPI anchored serine-threonine rich protein OS C. immitis\u201d, while from the \u201cuncharacterized protein of C. immitis\u201d, we selected a peptide for C. immitis and C. posadasii. These proteins arose from the 100 kDa antigenic fraction. From the protein \u201cfatty acid amide hydrolase 1 of C. posadasii\u201d that was identified from the 50 kDa antigenic fraction, a peptide was selected that recognized C. immitis and C. posadasii. In addition, the analysis of all the peptides (353) of each of the assembled proteins showed that only 35 had 100% identity with proteins of C. immitis and C. posadasii, one had 100% identity with only C. immitis, and one had 100% identity with only C. posadasii. These peptides can be used as diagnostic reagents, vaccines, and antifungals.<\/jats:p>","DOI":"10.3390\/molecules23123145","type":"journal-article","created":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T12:13:17Z","timestamp":1543579997000},"page":"3145","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Selection of Specific Peptides for Coccidioides spp. Obtained from Antigenic Fractions through SDS-PAGE and Western Blot Methods by the Recognition of Sera from Patients with Coccidioidomycosis"],"prefix":"10.3390","volume":"23","author":[{"given":"Esperanza","family":"Duarte Escalante","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad Universitaria No. 3000, M\u00e9xico Cd. Mx. 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3160-8698","authenticated-orcid":false,"given":"Mar\u00eda Guadalupe","family":"Fr\u00edas De Le\u00f3n","sequence":"additional","affiliation":[{"name":"Hospital Regional de Alta Especialidad de Ixtapaluca, Carretera Federal M\u00e9xico\u2014Puebla Km. 34.5, Pueblo de Zoquiapan, Ixtapaluca 56530, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz Gisela","family":"Mart\u00ednez Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad Universitaria No. 3000, M\u00e9xico Cd. Mx. 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Herrera","sequence":"additional","affiliation":[{"name":"Unidad de Servicios de Apoyo a la Investigaci\u00f3n y la Industria (USAII), Facultad de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad Universitaria No. 3000, M\u00e9xico Cd. Mx. 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Acosta Altamirano","sequence":"additional","affiliation":[{"name":"Hospital Regional de Alta Especialidad de Ixtapaluca, Carretera Federal M\u00e9xico\u2014Puebla Km. 34.5, Pueblo de Zoquiapan, Ixtapaluca 56530, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Cabello","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Enfermedades Respiratorias (INER), Calzada de Tlalpan 4502, Belisario Dom\u00ednguez Secci\u00f3n XVI, Tlalpan, M\u00e9xico Cd. Mx 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Palma","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Enfermedades Respiratorias (INER), Calzada de Tlalpan 4502, Belisario Dom\u00ednguez Secci\u00f3n XVI, Tlalpan, M\u00e9xico Cd. Mx 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda del Roc\u00edo","family":"Reyes Montes","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), Ciudad Universitaria No. 3000, M\u00e9xico Cd. Mx. 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1080\/15572536.2003.11833250","article-title":"Molecular and phenotypic description of Coccidioides posadasii sp. Nov., previously recognized as the non-California population of Coccidioides immitis","volume":"94","author":"Fisher","year":"2002","journal-title":"Mycologia"},{"key":"ref_2","first-page":"261","article-title":"Coccidioidomycosis in Argentina, 1892\u20132009","volume":"42","author":"Canteros","year":"2010","journal-title":"Rev. Argent. 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