{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T23:33:51Z","timestamp":1784590431314,"version":"3.55.0"},"reference-count":40,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T00:00:00Z","timestamp":1546387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Small molecules containing quinone and\/or oxime moieties have been found as promising anti-fungal agents. One of them is 4-AN, a recently reported potent anti-Candida compound, which inhibits the formation of hyphae, decreases the level of cellular phosphoproteome, and finally shows no toxicity towards human erythrocytes and zebrafish embryos. Here, further research on 4-AN is presented. The results revealed that the compound: (i) Kills Candida clinical isolates, including these with developed antibiotic resistance, (ii) affects mature biofilm, and (iii) moderately disrupts membrane permeability. Atomic force microscopy studies revealed a slight influence of 4-AN on the cell surface architecture. 4-AN was also shown to inhibit multiple various protein kinases, a characteristic shared by most of the ATP-competitive inhibitors. The presented compound can be used in novel strategies in the fight against candidiasis, and reversible protein phosphorylation should be taken into consideration as a target in designing these strategies.<\/jats:p>","DOI":"10.3390\/molecules24010153","type":"journal-article","created":{"date-parts":[[2019,1,3]],"date-time":"2019-01-03T03:36:30Z","timestamp":1546486590000},"page":"153","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Anti-Candida albicans Agent 4-AN Inhibits Multiple Protein Kinases"],"prefix":"10.3390","volume":"24","author":[{"given":"Maciej","family":"Mas\u0142yk","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Institute of Biotechnology, The John Paul II Catholic University of Lublin, ul. Konstantyn\u00f3w 1i, 20-708 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Janeczko","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Institute of Biotechnology, The John Paul II Catholic University of Lublin, ul. Konstantyn\u00f3w 1i, 20-708 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Martyna","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Institute of Biotechnology, The John Paul II Catholic University of Lublin, ul. Konstantyn\u00f3w 1i, 20-708 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u0142awomir","family":"Czernik","sequence":"additional","affiliation":[{"name":"Innovation Research Centre, Pope John Paul II State School of Higher Education in Biala Podlaska, Sidorska 95\/97, 21-500 Biala Podlaska, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2297-970X","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Tokarska-Rodak","sequence":"additional","affiliation":[{"name":"Institute of Health Sciences, Pope John Paul II State School of Higher Education in Biala Podlaska, Sidorska 95\/97, 21-500 Biala Podlaska, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Chwedczuk","sequence":"additional","affiliation":[{"name":"Innovation Research Centre, Pope John Paul II State School of Higher Education in Biala Podlaska, Sidorska 95\/97, 21-500 Biala Podlaska, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Foll-Josselin","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, USR3151 CNRS\/UPMC, Plateforme de criblage KISSf (Kinase Inhibitor Specialized Screening Facility), Station Biologique, Place Georges Teissier, F-29688 Roscoff, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Ruchaud","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, USR3151 CNRS\/UPMC, Plateforme de criblage KISSf (Kinase Inhibitor Specialized Screening Facility), Station Biologique, Place Georges Teissier, F-29688 Roscoff, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7186-7483","authenticated-orcid":false,"given":"St\u00e9phane","family":"Bach","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, USR3151 CNRS\/UPMC, Plateforme de criblage KISSf (Kinase Inhibitor Specialized Screening Facility), Station Biologique, Place Georges Teissier, F-29688 Roscoff, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5571-1593","authenticated-orcid":false,"given":"Oleg M.","family":"Demchuk","sequence":"additional","affiliation":[{"name":"Pharmaceutical Research Institute, Rydygiera Street 8, 01-793 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6870-845X","authenticated-orcid":false,"given":"Konrad","family":"Kubi\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Institute of Biotechnology, The John Paul II Catholic University of Lublin, ul. Konstantyn\u00f3w 1i, 20-708 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Pfaller, M.A., Andes, D.R., Diekema, D.J., Horn, D.L., Reboli, A.C., Rotstein, C., Franks, B., and Azie, N.E. (2014). Epidemiology and outcomes of invasive candidiasis due to non-albicans species of Candida in 2,496 patients: Data from the Prospective Antifungal Therapy (PATH) registry 2004\u20132008. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0101510"},{"key":"ref_2","unstructured":"Rex, J.H., Sobel, J.D., and Powderly, W.G. (1999). Cansida species. Antimicrobial therapy and vaccines, Williams & Wilkins Inc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.bcp.2016.11.019","article-title":"Antifungals","volume":"133","author":"Campoy","year":"2017","journal-title":"Biochem. 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