{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:25:29Z","timestamp":1790688329220,"version":"4.1.0"},"reference-count":52,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T00:00:00Z","timestamp":1547683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad La Salle","award":["SAL-05\/11"],"award-info":[{"award-number":["SAL-05\/11"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Infection from multidrug resistant bacteria has become a growing health concern worldwide, increasing the need for developing new antibacterial agents. Among the strategies that have been studied, biofilm inhibitors have acquired relevance as a potential source of drugs that could act as a complement for current and new antibacterial therapies. Based on the structure of 2-alkyl-3-hydroxy-4-quinolone and N-acylhomoserine lactone, molecules that act as mediators of quorum sensing and biofilm formation in Pseudomonas aeruginosa, we designed, prepared, and evaluated the biofilm inhibition properties of long chain amide derivatives of 2-amino-4-quinolone in Staphylococcus aureus and P. aeruginosa. All compounds had higher biofilm inhibition activity in P. aeruginosa than in S. aureus. Particularly, compounds with an alkyl chain of 12 carbons exhibited the highest inhibition of biofilm formation. Docking scores and molecular dynamics simulations of the complexes of the tested compounds within the active sites of proteins related to quorum sensing had good correlation with the experimental results, suggesting the diminution of biofilm formation induced by these compounds could be related to the inhibition of these proteins.<\/jats:p>","DOI":"10.3390\/molecules24020327","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T11:30:27Z","timestamp":1547724627000},"page":"327","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Synthesis, In Silico, and In Vitro Evaluation of Long Chain Alkyl Amides from 2-Amino-4-Quinolone Derivatives as Biofilm Inhibitors"],"prefix":"10.3390","volume":"24","author":[{"given":"Mariana Paola","family":"Espinosa-Vald\u00e9s","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad La Salle-M\u00e9xico. Av. Benjamin Franklin 45, Cuauht\u00e9moc, Mexico City 06140, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Borbolla-Alvarez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad La Salle-M\u00e9xico. Av. Benjamin Franklin 45, Cuauht\u00e9moc, Mexico City 06140, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Elena","family":"Delgado-Espinosa","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad La Salle-M\u00e9xico. Av. Benjamin Franklin 45, Cuauht\u00e9moc, Mexico City 06140, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Francisco","family":"S\u00e1nchez-Tejeda","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad La Salle-M\u00e9xico. Av. Benjamin Franklin 45, Cuauht\u00e9moc, Mexico City 06140, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anabelle","family":"Cer\u00f3n-Nava","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad La Salle-M\u00e9xico. Av. Benjamin Franklin 45, Cuauht\u00e9moc, Mexico City 06140, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osvaldo Javier","family":"Quintana-Romero","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e1nica, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional (CINVESTAV-IPN), Av. Instituto Polit\u00e9cnico Nacional 2508, Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2799-0764","authenticated-orcid":false,"given":"Armando","family":"Ariza-Castolo","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e1nica, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional (CINVESTAV-IPN), Av. Instituto Polit\u00e9cnico Nacional 2508, Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego Fernando","family":"Garc\u00eda-Del R\u00edo","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad La Salle-M\u00e9xico. Av. Benjamin Franklin 45, Cuauht\u00e9moc, Mexico City 06140, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8449-0806","authenticated-orcid":false,"given":"Marco A.","family":"Loza-Mej\u00eda","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad La Salle-M\u00e9xico. Av. Benjamin Franklin 45, Cuauht\u00e9moc, Mexico City 06140, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.biotechadv.2012.10.004","article-title":"Quorum sensing inhibitors: An overview","volume":"31","author":"Kalia","year":"2013","journal-title":"Biotechnol. 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