{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:33:59Z","timestamp":1760240039270,"version":"build-2065373602"},"reference-count":16,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,2,22]],"date-time":"2019-02-22T00:00:00Z","timestamp":1550793600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81473101, 81001401"],"award-info":[{"award-number":["81473101, 81001401"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Natural Science Foundation of Hebei Province","award":["H2017406049"],"award-info":[{"award-number":["H2017406049"]}]},{"name":"the Key Programs of Education Department of Hebei Province","award":["ZD2018080"],"award-info":[{"award-number":["ZD2018080"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A procedure to measure the serum concentration of glycogen phosphorylase during acute myocardial infarction is presented. This method was based on the synthesis of photoaffinity probes, and used the semiquantitative protein electrophoretic mobility shift technique. Three novel photoaffinity probes bearing different secondary tags were synthesized. Their potency was evaluated in an enzyme inhibition assay against rabbit muscle glycogen phosphorylase a (RMGPa). The inhibitory activity of probe 1 was only 100-fold less potent than the mother compound CP-320626. The photoaffinity labeling experiments were also performed, and a protein with molecular weight (MW) of about 90\u2013100 kDa, which was consistent with the MW of GP, was clearly labeled by probe 1. A semiquantitative evaluation of the GP level in serum with probe 1 was also performed. The results showed that the protein band with a MW of about 90\u2013100 kDa was tagged, and the concentration of the protein in serum was found to be between 25 and 50 ng\/mL. Mass spectrometric analysis revealed that alpha-1,4 glucan phosphorylase (GPMM) was well-preserved in the bands.<\/jats:p>","DOI":"10.3390\/molecules24040798","type":"journal-article","created":{"date-parts":[[2019,2,22]],"date-time":"2019-02-22T11:26:14Z","timestamp":1550834774000},"page":"798","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Design, Synthesis, and Use of Novel Photoaffinity Probes in Measuring the Serum Concentration of Glycogen Phosphorylase"],"prefix":"10.3390","volume":"24","author":[{"given":"Yuchao","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Research and Development of Hebei Province, Institute of Traditional Chinese Medicine, Chengde Medical University, Chengde 067000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youde","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Research and Development of Hebei Province, Institute of Traditional Chinese Medicine, Chengde Medical University, Chengde 067000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiwei","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Research and Development of Hebei Province, Institute of Traditional Chinese Medicine, Chengde Medical University, Chengde 067000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengjun","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, Chengde Medical University, Chengde 067000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangxin","family":"Miao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Research and Development of Hebei Province, Institute of Traditional Chinese Medicine, Chengde Medical University, Chengde 067000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Traditional Chinese Medicine Research and Development of Hebei Province, Institute of Traditional Chinese Medicine, Chengde Medical University, Chengde 067000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,2,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/BF00240061","article-title":"Glycogen phosphorylase isoenzyme BB in diagnosis of myocardial ischaemic injury and infarction","volume":"160","author":"Krause","year":"1996","journal-title":"Mol. 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