{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T06:41:54Z","timestamp":1780555314181,"version":"3.54.1"},"reference-count":47,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2019,3,15]],"date-time":"2019-03-15T00:00:00Z","timestamp":1552608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>A method for detecting the organophosphorus pesticides residue and aflatoxins in China herbal tea has been developed by UPLC-MS\/MS coupled with vortex-assisted dispersive liquid-liquid microextraction (DLLME). The extraction conditions for vortex-assisted DLLME extraction were optimized using single-factor experiments and response surface design. The optimum conditions for the experiment were the pH 5.1, 347 \u00b5L of chloroform (extraction solvent) and 1614 \u00b5L of acetonitrile (dispersive solvent). Under the optimum conditions, the targets were good linearity in the range of 0.1 \u00b5g\/L\u201325 \u00b5g\/L and the correlation coefficient above 0.9998. The mean recoveries of all analytes were in the ranged from 70.06%\u2013115.65% with RSDs below 8.54%. The detection limits were in the range of 0.001 \u00b5g\/L\u20130.01\u00b5g\/L. The proposed method is a fast and effective sample preparation with good enrichment and extraction efficiency, which can simultaneously detect pesticides and aflatoxins in China herbal tea.<\/jats:p>","DOI":"10.3390\/molecules24061029","type":"journal-article","created":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T04:06:55Z","timestamp":1552882015000},"page":"1029","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["A Vortex-Assisted Dispersive Liquid-Liquid Microextraction Followed by UPLC-MS\/MS for Simultaneous Determination of Pesticides and Aflatoxins in Herbal Tea"],"prefix":"10.3390","volume":"24","author":[{"given":"Rui","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture &amp; Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Chao","family":"Tan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture &amp; Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke-Cheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Shenzhen Noposion Agrochemical Co. Ltd., Shenzhen 510640, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture &amp; Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture &amp; Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Long","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture &amp; Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9067-2630","authenticated-orcid":false,"given":"Cheng-Lan","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture &amp; Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s13002-018-0257-0","article-title":"Ethnobotanical survey of plant species for herbal tea in a Yao autonomous county (Jianghua, China): Results of a 2-year study of traditional medicinal markets on the Dragon Boat Festival","volume":"14","author":"Jin","year":"2018","journal-title":"J. Ethnobiol. Ethnomed."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.jep.2018.04.026","article-title":"A billion cups: The diversity, traditional uses, safety issues and potential of Chinese herbal teas","volume":"222","author":"Fu","year":"2018","journal-title":"J. Ethnopharmacol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/1746-4269-9-82","article-title":"Ethnobotanical survey of cooling herbal drinks from southern China","volume":"9","author":"Liu","year":"2013","journal-title":"J. Ethnobiol. Ethnomed."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jep.2004.11.003","article-title":"Plants known as t\u00e9 in Spain: An ethno-pharmaco-botanical review","volume":"98","author":"Blanco","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_5","first-page":"259","article-title":"Systematic Research on Chinese Non-Camellia Tea","volume":"15","author":"Han","year":"2013","journal-title":"Mod. Chin. Med."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Li, S., Li, S.K., Li, H.B., Xu, X.R., Deng, G.F., and Xu, D.P. (2014). Antioxidant Capacities of Herbal Infusions, South China Sea Institute of Oceanology, Chinese Academy of Sciences.","DOI":"10.1016\/B978-0-12-404738-9.00005-2"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.jep.2015.03.028","article-title":"Herbs and herbal combinations used to treat suspected malaria in Bo, Sierra Leone","volume":"166","author":"Ranasinghe","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s12231-014-9296-1","article-title":"Health in a Pot-The Ethnobotany of Emolientes and Emolienteros in Peru","volume":"69","author":"Bussmann","year":"2015","journal-title":"Econ. Bot."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.appet.2014.03.024","article-title":"Beverage and culture. \u201cZhourat\u201d, a multivariate analysis of the globalization of an herbal tea from the Middle East","volume":"79","author":"Rivera","year":"2014","journal-title":"Appetite"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jep.2004.11.003","article-title":"Plants known as t\u00e9 in Spain: an ethno-pharmaco-botanical review","volume":"98","author":"Pardo","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s00604-009-0229-0","article-title":"Simultaneous determination of flavonoids and phenolic acids in Chinese herbal tea by beta-cyclodextrin based capillary zone electrophoresis","volume":"167","author":"Chi","year":"2009","journal-title":"Microchim. Acta"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1080\/02652030600986198","article-title":"Multiresidue pesticide analysis of the medicinal plant Origanum syriacum","volume":"24","author":"Hajjo","year":"2007","journal-title":"Food Addit. Contam."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1002\/ptr.1694","article-title":"Systematic evaluation of organochlorine pesticide residues in Chinese materia medica","volume":"19","author":"Leung","year":"2010","journal-title":"Phytother. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1002\/jssc.201600570","article-title":"Simultaneous determination of 35 ultra-trace level organophosphorus pesticide residues in Sanjie Zhentong capsules of traditional Chinese medicine using ultra high performance liquid chromatography with tandem mass spectrometry","volume":"40","author":"Li","year":"2017","journal-title":"J. Sep. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.etap.2010.01.006","article-title":"Neurotoxic effects in patients poisoned with organophosphorus pesticides","volume":"29","year":"2010","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1021\/acs.jafc.5b05369","article-title":"Sensitive detection of organophosphorus pesticides in medicinal plants using ultrasound-assisted dispersive liquid\u2013liquid microextraction combined with sweeping micellar electrokinetic chromatography","volume":"64","author":"Wei","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.jchromb.2018.06.012","article-title":"Determination of aflatoxin and zearalenone analogs in edible and medicinal herbs using a group-specific immunoaffinity column coupled to ultra-high-performance liquid chromatography with tandem mass spectrometry","volume":"1092","author":"Sun","year":"2018","journal-title":"J. Chromatogr. B"},{"key":"ref_18","unstructured":"International Agency for Research on Cancer (IARC) (2010). IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Working Group Evaluation Carcinogenic Risks Humans I, IARC."},{"key":"ref_19","unstructured":"International Agency for Research on Cancer (IARC) (2010). Aflatoxins: B1, B2, G1, G2, M1, 245\u2013395. IARC Monographs on the Evaluation of Carcinogenic Risks of Chemicals to Humans, IARC."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chroma.2016.01.034","article-title":"Analytical methods for the endocrine disruptor compounds determination in environmental water samples","volume":"1434","author":"Locatelli","year":"2016","journal-title":"J. Chromotogr. A"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1039\/C4AY02553A","article-title":"Simple one-step preconcentration and cleanup with a micellar system for high performance liquid chromatography determination of pyrethroids in traditional Chinese medicine","volume":"7","author":"Chen","year":"2015","journal-title":"Anal. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"210","DOI":"10.3724\/SP.J.1009.2009.00210","article-title":"Simultaneous determination of 26 pesticide residues in 5 Chinese medicinal materials using solid-phase extraction and GC-ECD Method","volume":"7","author":"Guo","year":"2009","journal-title":"Chin. J. Nat. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.foodchem.2018.11.130","article-title":"Three approaches to minimize matrix effects in residue analysis of multiclass pesticides in dried complex matrices using gas chromatography tandem mass spectrometry","volume":"279","author":"Rutkowska","year":"2019","journal-title":"Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"823","DOI":"10.2116\/analsci.31.823","article-title":"Multi-residue method for determination of selected neonicotinoid insecticides in traditional Chinese medicine using modified dispersive solid-phase extraction combined with ultra-performance liquid chromatography tandem mass spectrometry","volume":"31","author":"Qin","year":"2015","journal-title":"Anal. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1134\/S1061934814060045","article-title":"Simultaneous determination of several mycotoxins by rapid immunofiltration assay","volume":"69","author":"Burmistrova","year":"2014","journal-title":"J. Anal. Chem."},{"key":"ref_26","first-page":"334","article-title":"Determination of aflatoxin M1 in milk by high-performance liquid chromatography in Mashhad (North East of Iran)","volume":"29","author":"Mohamadi","year":"2012","journal-title":"Toxicol. Ind. Health"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.aca.2010.02.009","article-title":"An ultra-high-performance liquid chromatography-tandem mass spectrometry method for simultaneous determination of aflatoxins B1, B2, G1, G2, M1 and M2 in traditional Chinese medicines","volume":"664","author":"Han","year":"2010","journal-title":"Anal. Chim. Acta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.aca.2018.02.069","article-title":"Synthesis and application of molecularly imprinted silica for the selective extraction of some polar organophosphorus pesticides from almond oil","volume":"1018","author":"Boulanouar","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s12161-018-1335-6","article-title":"Analytical method validation for determining organophosphorus pesticides in baby foods by a modified liquid\u2013liquid microextraction method and gas chromatography\u2013ion trap\/mass spectrometry analysis","volume":"12","author":"Notardonato","year":"2019","journal-title":"Food Anal. Method"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6776","DOI":"10.3390\/molecules19056776","article-title":"Applications of Liquid-Phase Microextraction in the Sample Preparation of Environmental Solid Samples","volume":"19","author":"Prosen","year":"2014","journal-title":"Molecules"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Kissoudi, M., and Samanidou, V. (2018). Recent advances in applications of ionic liquids in miniaturized microextraction techniques. Molecules, 23.","DOI":"10.3390\/molecules23061437"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1002\/pca.2737","article-title":"Use of innovative (Micro)Extraction techniques to CharacteriseHarpagophytum procumbens root and its commercial food supplements","volume":"29","author":"Diuzheva","year":"2018","journal-title":"Phytochem. Anal."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Moyakao, K., Santaladchaiyakit, Y., Srijaranai, S., and Vichapong, J. (2018). Preconcentration of trace neonicotinoid insecticide residues using vortex-assisted dispersive micro solid-phase extraction with montmorillonite as an efficient sorbent. Molecules, 23.","DOI":"10.3390\/molecules23040883"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2528","DOI":"10.3390\/molecules18032528","article-title":"Identification of Alternaria alternata mycotoxins by LC-SPE-NMR and their cytotoxic effects to soybean (Glycine max) cell suspension culture","volume":"18","author":"Daolio","year":"2013","journal-title":"Molecules"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.aca.2013.02.043","article-title":"Highly sensitive and selective organophosphate screening in twelve commodities of fruits, vegetables and herbal medicines by dispersive liquid\u2013liquid microextraction","volume":"775","author":"Ho","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1002\/jsfa.7978","article-title":"Simultaneous determination of six mycotoxins in peanut by high-performance liquid chromatography with a fluorescence detector","volume":"97","author":"Chen","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chroma.2013.04.053","article-title":"Recent development and applications of dispersive liquid\u2013liquid microextraction","volume":"1295","author":"Yan","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.trac.2010.03.016","article-title":"Dispersive liquid-liquid microextraction for determination of organic analytes","volume":"29","year":"2010","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.microc.2015.11.003","article-title":"Determination of 27 pesticides in wine by dispersive liquid\u2013liquid microextraction and gas chromatography\u2013mass spectrometry","volume":"126","author":"Chen","year":"2016","journal-title":"Microchem. J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6603","DOI":"10.1016\/j.chroma.2011.07.054","article-title":"Dispersive liquid\u2013liquid microextraction using non-chlorinated, lighter than water solvents for gas chromatography\u2013mass spectrometry determination of fungicides in wine","volume":"1218","author":"Ramil","year":"2011","journal-title":"J. Chromotogr. A"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1016\/j.trac.2011.04.014","article-title":"Dispersive liquid-liquid microextraction","volume":"30","year":"2011","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7243","DOI":"10.1039\/C7RA11896A","article-title":"Vortex-assisted low density solvent and surfactant based dispersive liquid-liquid microextraction for sensitive spectrophotometric determination of cobalt","volume":"8","author":"Chaiyamate","year":"2018","journal-title":"RSC Adv."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.foodchem.2014.04.033","article-title":"Application of ionic liquid-based dispersive liquid\u2013liquid microextraction for the analysis of ochratoxin A in rice wines","volume":"161","author":"Lai","year":"2014","journal-title":"Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1016\/j.talanta.2010.06.008","article-title":"Temperature-assisted ionic liquid dispersive liquid\u2013liquid microextraction combined with high performance liquid chromatography for the determination of anthraquinones in Radix et Rhizoma Rhei samples","volume":"82","author":"Zhang","year":"2010","journal-title":"Talanta"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.jchromb.2007.03.017","article-title":"Reconsideration of sample pH adjustment in bioanalytical liquid\u2013liquid extraction of ionisable compounds","volume":"853","author":"Hendriks","year":"2007","journal-title":"J. Chromatogr. B"},{"key":"ref_46","first-page":"15","article-title":"Matrix effect in gas chromatographic determination of insecticides and fungicides in vegetables","volume":"84","author":"Fotopoulou","year":"2004","journal-title":"Int. J. Environ. Chem."},{"key":"ref_47","first-page":"12","article-title":"Commission Regulation (EC) No 401\/2006 of 23 February 2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs","volume":"70","author":"Commission","year":"2006","journal-title":"J. Eur. Union"}],"container-title":["Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1420-3049\/24\/6\/1029\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:39:03Z","timestamp":1760186343000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1420-3049\/24\/6\/1029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,15]]},"references-count":47,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2019,3]]}},"alternative-id":["molecules24061029"],"URL":"https:\/\/doi.org\/10.3390\/molecules24061029","relation":{},"ISSN":["1420-3049"],"issn-type":[{"value":"1420-3049","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,15]]}}}