{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:34:36Z","timestamp":1792182876286,"version":"4.4.0"},"reference-count":50,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,3,30]],"date-time":"2019-03-30T00:00:00Z","timestamp":1553904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Our previous works have shown that dioscin, a natural product, has various pharmacological activities, however, its role in brain aging has not been reported. In the present study, in vitro H2O2-treated PC12 cells and in vivo d-galactose-induced aging rat models were used to evaluate the neuroprotective effect of dioscin on brain aging. The results showed that dioscin increased cell viability and protected PC12 cells against oxidative stress through decreasing reactive oxygen species (ROS) and lactate dehydrogenase (LDH) levels. In vivo, dioscin markedly improved the spatial learning ability and memory of aging rats, reduced the protein carbonyl content and aging cell numbers, restored the levels of superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and nitric oxide synthase (NOS) in brain tissue, and reversed the histopathological structure changes of nerve cells. Mechanism studies showed that dioscin markedly adjusted the MAPK and Nrf2\/ARE signalling pathways to decrease oxidative stress. Additionally, dioscin also significantly decreased inflammation by inhibiting the mRNA or protein levels of TNF-\u03b1, IL-1\u03b2, IL-6, CYP2E1 and HMGB1. Taken together, these results indicate that dioscin showed neuroprotective effect against brain aging via decreasing oxidative stress and inflammation, which should be developed as an efficient candidate in clinical to treat brain aging in the future.<\/jats:p>","DOI":"10.3390\/molecules24071247","type":"journal-article","created":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T03:21:26Z","timestamp":1554175286000},"page":"1247","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Neuroprotective Effect of Dioscin on the Aging Brain"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3168-4332","authenticated-orcid":false,"given":"Yan","family":"Qi","sequence":"first","affiliation":[{"name":"College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruomiao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianhong","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youwei","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Han","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyong","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, China"},{"name":"Key Laboratory for Basic and Applied Research on Pharmacodynamic Substances of Traditional Chinese Medicine of Liaoning Province, Dalian Medical University, Dalian 116044, China"},{"name":"National-Local Joint Engineering Research Center for Drug Development (R&amp;D) of Neurodegenerative Diseases, Dalian Medical University, Dalian 116044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,30]]},"reference":[{"key":"ref_1","unstructured":"United Nations (2019, February 11). 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