{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T05:46:05Z","timestamp":1762407965282,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802014"],"award-info":[{"award-number":["31802014"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Chongqing, China","award":["cstc2018jcyjAX0487, cstc2018jcyjAX0211, cstc2016jcyjA0524"],"award-info":[{"award-number":["cstc2018jcyjAX0487, cstc2018jcyjAX0211, cstc2016jcyjA0524"]}]},{"DOI":"10.13039\/100012546","name":"Chongqing Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["XmT2018006"],"award-info":[{"award-number":["XmT2018006"]}],"id":[{"id":"10.13039\/100012546","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Bombyx mori nucleopolyhedrovirus (BmNPV) is the most prevalent threat to silkworms. Hence, there is a need for antiviral agents in sericulture. The PI3K-Akt pathway is essential for the efficient replication of the baculovirus. In an attempt to screen antiviral drugs against BmNPV, we summarized the commercial compounds targeting PI3K-Akt and selected the following seven oral drugs for further analyses: afuresertib, AZD8835, AMG319, HS173, AS605240, GDC0941, and BEZ235. Cell viability assay revealed that the cytotoxicity of these drugs at 10 \u00b5M concentration was not strong. Viral fluorescence observation and qPCR analysis showed that these candidate drugs significantly inhibited BmNPV in BmE cells. Only AMG319 and AZD8835 inhibited viral proliferation in silkworm larvae. The mortality of AZD8835-treated silkworms was lower than that of the control silkworms. Western blotting showed that AMG319 and AZD8835 decreased p-Akt expression after BmNPV infection. These results suggest that AZD8835 has application potential in sericulture.<\/jats:p>","DOI":"10.3390\/molecules24071260","type":"journal-article","created":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T03:21:26Z","timestamp":1554175286000},"page":"1260","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Screening of PI3K-Akt-targeting Drugs for Silkworm against Bombyx mori Nucleopolyhedrovirus"],"prefix":"10.3390","volume":"24","author":[{"given":"Bingbing","family":"Wang","sequence":"first","affiliation":[{"name":"Biological Science Research Center, Southwest University, Chongqing 400716, China"},{"name":"Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Jiang","sequence":"additional","affiliation":[{"name":"Biological Science Research Center, Southwest University, Chongqing 400716, China"},{"name":"Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huizhen","family":"Guo","sequence":"additional","affiliation":[{"name":"Biological Science Research Center, Southwest University, Chongqing 400716, China"},{"name":"Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Biological Science Research Center, Southwest University, Chongqing 400716, China"},{"name":"Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumei","family":"Wang","sequence":"additional","affiliation":[{"name":"Biological Science Research Center, Southwest University, Chongqing 400716, China"},{"name":"Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enyu","family":"Xie","sequence":"additional","affiliation":[{"name":"Biological Science Research Center, Southwest University, Chongqing 400716, China"},{"name":"Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9263-2886","authenticated-orcid":false,"given":"Qingyou","family":"Xia","sequence":"additional","affiliation":[{"name":"Biological Science Research Center, Southwest University, Chongqing 400716, China"},{"name":"Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ibmb.2014.02.003","article-title":"The progress and future of enhancing antiviral capacity by transgenic technology in the silkworm Bombyx mori","volume":"48","author":"Jiang","year":"2014","journal-title":"Insect Biochem. 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