{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:50:02Z","timestamp":1790373002798,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T00:00:00Z","timestamp":1554163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007161","name":"Secretar\u00eda de Investigaci\u00f3n y Posgrado, Instituto Polit\u00e9cnico Nacional","doi-asserted-by":"publisher","award":["20170496"],"award-info":[{"award-number":["20170496"]}],"id":[{"id":"10.13039\/501100007161","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007161","name":"Secretar\u00eda de Investigaci\u00f3n y Posgrado, Instituto Polit\u00e9cnico Nacional","doi-asserted-by":"publisher","award":["20180034"],"award-info":[{"award-number":["20180034"]}],"id":[{"id":"10.13039\/501100007161","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004881","name":"Instituto Mexicano del Seguro Social","doi-asserted-by":"publisher","award":["FIS\/IMSS\/PROT\/G18\/1805"],"award-info":[{"award-number":["FIS\/IMSS\/PROT\/G18\/1805"]}],"id":[{"id":"10.13039\/501100004881","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["220007"],"award-info":[{"award-number":["220007"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Stress is an important factor in the etiology of some illnesses such as gastric ulcers and depression. Castilleja tenuiflora Benth. (Orobanchaceae) is used in Mexican traditional medicine for the treatment of gastrointestinal diseases and nervous disorders. Previous studies indicated that organic extracts from C. tenuiflora had gastroprotective effects and antidepressant activity. In this study, we aimed to evaluate the gastroprotective and antidepressant activity of fractions and isolated compounds from the methanolic extract (MECt) of C. tenuiflora in stressed mice. Chromatographic fractionation of MECt produced four fractions (FCt-1, FCt-2, CFt-3, and FCt-4) as well as four bioactive compounds which were identified using TLC, HPLC and NMR analyses. The cold restraint stress (CRS)-induced gastric ulcer model followed by the tail suspension test and the forced swim test were used to evaluate the gastroprotective effect and antidepressant activity of the extract fractions. FCt-2 and FCt-3 at 100 mg\/kg had significant gastroprotective and antidepressant effects. All isolated compounds (verbascoside, teniufloroside and mixture geniposide\/ musseanoside) displayed gastroprotective effects and antidepressant activity at 1 or 2 mg\/kg. The above results allow us to conclude that these polyphenols and iridoids from C. tenuiflora are responsible for the gastroprotective and antidepressant effects.<\/jats:p>","DOI":"10.3390\/molecules24071292","type":"journal-article","created":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T03:39:28Z","timestamp":1554262768000},"page":"1292","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["In Vivo Gastroprotective and Antidepressant Effects of Iridoids, Verbascoside and Tenuifloroside from Castilleja tenuiflora Benth"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2202-8584","authenticated-orcid":false,"given":"Ricardo","family":"L\u00f3pez-Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Centro de Desarrollo de Productos Bi\u00f3ticos, Instituto Polit\u00e9cnico Nacional, Col. San Isidro, Yautepec, Morelos C.P. 62731, Mexico"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec, Morelos C.P. 62790, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maribel","family":"Herrera-Ruiz","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec, Morelos C.P. 62790, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7479-9332","authenticated-orcid":false,"given":"Gabriela","family":"Trejo-Tapia","sequence":"additional","affiliation":[{"name":"Centro de Desarrollo de Productos Bi\u00f3ticos, Instituto Polit\u00e9cnico Nacional, Col. San Isidro, Yautepec, Morelos C.P. 62731, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca Eda","family":"Dom\u00ednguez-Mendoza","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos C.P. 62209, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3693-1670","authenticated-orcid":false,"given":"Manas\u00e9s","family":"Gonz\u00e1lez-Cortazar","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec, Morelos C.P. 62790, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2233-5958","authenticated-orcid":false,"given":"Alejandro","family":"Zamilpa","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec, Morelos C.P. 62790, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.5056\/jnm14116","article-title":"The effect of emotional stress and depression on the prevalence of digestive diseases","volume":"21","author":"Lee","year":"2015","journal-title":"J. Neurogastroenterol. Motil."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhang, S., Xu, Z., Gao, Y., Wu, Y., Li, Z., Liu, H., and Zhang, C. (2012). 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