{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T19:09:53Z","timestamp":1788376193376,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T00:00:00Z","timestamp":1558483200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004504","name":"Lietuvos Mokslo Taryba","doi-asserted-by":"publisher","award":["09.3.3.-LMT-K-712-10-0095"],"award-info":[{"award-number":["09.3.3.-LMT-K-712-10-0095"]}],"id":[{"id":"10.13039\/501100004504","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>Acinetobacter baumannii is a nosocomial human pathogen of increasing concern due to its multidrug resistance profile. The outer membrane protein A (OmpA) is an abundant bacterial cell surface component involved in A. baumannii pathogenesis. It has been shown that the C-terminal domain of OmpA is located in the periplasm and non-covalently associates with the peptidoglycan layer via two conserved amino acids, thereby anchoring OmpA to the cell wall. Here, we investigated the role of one of the respective residues, D268 in OmpA of A. baumannii clinical strain Ab169, on its virulence characteristics by complementing the \u0394ompA mutant with the plasmid-borne ompAD268A allele. We show that while restoring the impaired biofilm formation of the \u0394ompA strain, the Ab169ompAD268A mutant tended to form bacterial filaments, indicating the abnormalities in cell division. Moreover, the Ab169 OmpA D268-mediated association to peptidoglycan was required for the manifestation of twitching motility, desiccation resistance, serum-induced killing, adhesion to epithelial cells and virulence in a nematode infection model, although it was dispensable for the uptake of \u03b2-lactam antibiotics by outer membrane vesicles. Overall, the results of this study demonstrate that the OmpA C-terminal domain-mediated association to peptidoglycan is critical for a number of virulent properties displayed by A. baumannii outside and within the host.<\/jats:p>","DOI":"10.3390\/molecules24101972","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T03:22:03Z","timestamp":1558581723000},"page":"1972","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The Mutation of Conservative Asp268 Residue in the Peptidoglycan-Associated Domain of the OmpA Protein Affects Multiple Acinetobacter baumannii Virulence Characteristics"],"prefix":"10.3390","volume":"24","author":[{"given":"J\u016brat\u0117","family":"Skerni\u0161kyt\u0117","sequence":"first","affiliation":[{"name":"Institute of Biosciences, Life Sciences Center, Vilnius University, Saul\u0117tekio ave. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3949-9219","authenticated-orcid":false,"given":"Emilija","family":"Karazijait\u0117","sequence":"additional","affiliation":[{"name":"Institute of Biosciences, Life Sciences Center, Vilnius University, Saul\u0117tekio ave. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Deschamps","sequence":"additional","affiliation":[{"name":"Micalis Institute, INRA, AgroParisTech, Universit\u00e9 Paris-Saclay, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renatas","family":"Krasauskas","sequence":"additional","affiliation":[{"name":"Institute of Biosciences, Life Sciences Center, Vilnius University, Saul\u0117tekio ave. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Briandet","sequence":"additional","affiliation":[{"name":"Micalis Institute, INRA, AgroParisTech, Universit\u00e9 Paris-Saclay, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edita","family":"Su\u017eied\u0117lien\u0117","sequence":"additional","affiliation":[{"name":"Institute of Biosciences, Life Sciences Center, Vilnius University, Saul\u0117tekio ave. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1001\/archsurg.2011.41","article-title":"Increases in mortality, length of stay, and cost associated with hospital-acquired infections in trauma patients","volume":"146","author":"Glance","year":"2011","journal-title":"Arch Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.vhri.2013.03.002","article-title":"Assessment of Costs Associated with Hospital-Acquired Infections in a Private Tertiary Care Hospital in India","volume":"2","author":"Tiwari","year":"2013","journal-title":"Value Health Reg. 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