{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:42:30Z","timestamp":1760240550368,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T00:00:00Z","timestamp":1563321600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Chinese Academy of Sciences (CAS) \u2018Light of West China\u2019 Program","award":["2018XBZG_XBQNXZ_A_005"],"award-info":[{"award-number":["2018XBZG_XBQNXZ_A_005"]}]},{"name":"the Macao Science and Technology Development Fund","award":["052\/2013\/A2"],"award-info":[{"award-number":["052\/2013\/A2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecules"],"abstract":"<jats:p>We have developed a new competitive protein binding assay (CPBA) based on human serum albumin functionalized silicon dioxide nanoparticles (nano-SiO2-HSA) that can be used for naproxen determination in urine. Compared with a conventional multi-well reaction plate, nano-SiO2 with a high surface-area-to-volume ratio could be introduced as a stationary phase, markedly improving the analytical performance. Nano-SiO2-HSA and horseradish peroxidase-labeled-naproxen (HRP-naproxen) were prepared for the present CPBA method. In this study, a direct competitive binding to nano-SiO2-HSAwas performed between the free naproxen in the sample and HRP-naproxen. Thus, the catalytic color reactions were investigated on an HRP\/3,3\u20325,5\u2032-tetramethylbenzidine (TMB)\/H2O2 system by the HRP-naproxen\/nano-SiO2-HSA composite for quantitative measurement via an ultraviolet spectrophotometer. A series of validation experiments indicated that our proposed methods can be applied satisfactorily to the determination of naproxen in urine samples. As a proof of principle, the newly developed nano-CPBA method for the quantification of naproxen in urine can be expected to have the advantages of low costs, fast speed, high accuracy, and relatively simple instrument requirements. Our method could be capable of expanding the analytical applications of nanomaterials and of determining other small-molecule compounds from various biological samples.<\/jats:p>","DOI":"10.3390\/molecules24142593","type":"journal-article","created":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T02:44:03Z","timestamp":1563331443000},"page":"2593","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Competitive Protein Binding Assay of Naproxen by Human Serum Albumin Functionalized Silicon Dioxide Nanoparticles"],"prefix":"10.3390","volume":"24","author":[{"given":"Qian-Long","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy, Chengdu Medical College, Chengdu 610500, China"},{"name":"Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China"},{"name":"State Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Chengdu Medical College, Chengdu 610500, China"},{"name":"State Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Liang","sequence":"additional","affiliation":[{"name":"Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geng-Ting","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Sheng","family":"Ding","sequence":"additional","affiliation":[{"name":"Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9685-9180","authenticated-orcid":false,"given":"Lin-Sen","family":"Qing","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Chengdu Medical College, Chengdu 610500, China"},{"name":"Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1002\/art.1780390222","article-title":"Steroidal Anti-inflammatory drug-induced enteropathy and severe chronic anemia in a patient with rheumatoid arthritis","volume":"39","author":"Davies","year":"1996","journal-title":"Arthritis Rheumatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s40265-015-0521-8","article-title":"Sumatriptan\/naproxen sodium: A review in migraine","volume":"76","author":"Syed","year":"2016","journal-title":"Drugs"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"241","DOI":"10.2165\/00003495-197918040-00001","article-title":"Naproxen up to date: A review of its pharmacological properties and therapeutic efficacy and use in rheumatic diseases and pain states","volume":"18","author":"Brogden","year":"1979","journal-title":"Drugs"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S0950-3579(88)80021-9","article-title":"Side-effects of non-steroidal anti-inflammatory drugs","volume":"2","author":"Henry","year":"1988","journal-title":"Bailli\u00e8re\u2019s Clin. 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